Identical Pyoverdines from Pseudomonas fluorescens 9AW and from Pseudomonas putida 9BW

Budzikiewicz, H.; Kilz, S.; Taraz, K.; Meyer, Jean Marie

doi:10.1515/znc-1997-11-1202

Cited by 21 publications

(11 citation statements)

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“…Even more surprising are the identical characteristics of siderophores produced by strains of P. syringae and P. viridiflava. Production of identical pyoverdins by different Pseudomonas species has been reported previously for borderline species (3,5,7,16). Similar results were obtained in this study since the genomic cluster containing P. syringae pv.…”

Section: Discussionsupporting

confidence: 92%

Production and Comparison of Peptide Siderophores from Strains of Distantly Related Pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352

Bultreys

Gheysen

2000

Appl Environ Microbiol

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The production of peptide siderophores and the variation in siderophore production among strains of Pseudomonas syringae and Pseudomonas viridiflava were investigated. An antibiose test was used to select a free amino acid-containing agar medium favorable for production of fluorescent siderophores by two P. syringae strains. A culture technique in which both liquid and solid asparagine-containing culture media were used proved to be reproducible and highly effective for inducing production of siderophores in a liquid medium by the fluorescent Pseudomonas strains investigated. Using asparagine as a carbon source appeared to favor siderophore production, and relatively high levels of siderophores were produced when certain amino acids were used as the sole carbon and energy sources. Purified chelated siderophores of strains of P. syringae pv. syringae, P. syringae pv. aptata, P. syringae pv. morsprunorum, P. syringae pv. tomato, and P. viridiflava had the same amino acid composition and spectral characteristics and were indiscriminately used by these strains. In addition, nonfluorescent strains of P. syringae pv. aptata and P. syringae pv. morsprunorum were able to use the siderophores in biological tests. Our results confirmed the proximity of P. syringae and P. viridiflava; siderotyping between pathovars of P. syringae was not possible. We found that the spectral characteristics of the chelated peptide siderophores were different from the spectral characteristics of typical pyoverdins. Our results are discussed in relation to the ecology of the organisms and the conditions encountered on plant surfaces.The species Pseudomonas syringae contains all of the phytopathogenic and oxidase-negative fluorescent pseudomonads except Pseudomonas viridiflava (27,38). P. syringae is divided into 57 pathovars that are pathogenic for numerous monocot and dicot crops (13). P. syringae strains are well adapted to conditions on plant surfaces. A better understanding of the ecological benefits of these pathogens is necessary if new and efficient methods of biological control are to be developed. One of these benefits could be the production of peptide siderophores in iron-deficient environments (8,33). In general, the peptide siderophores produced by fluorescent Pseudomonas strains are pyoverdins (2, 3). All pyoverdins contain the same quinoline chromophore, a peptide chain, and a dicarboxylic acid (or the corresponding amide) connected to the chromophore. The peptide chain is always the same for a given strain but is different in different strains and species (2). Two partially characterized peptide siderophores of P. syringae that have been described (8, 33) have Fe(III)-binding constants at pH 7.0 of about 1 ϫ 10 25 . These values are 10 times higher than the values obtained for pyoverdins produced by saprophytic fluorescent Pseudomonas strains (8, 33). Therefore, it would be interesting to know whether production of these molecules is common in P. syringae strains and whether the molecules are effectively produced on plant surf...

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Section: Discussionsupporting

confidence: 92%

Production and Comparison of Peptide Siderophores from Strains of Distantly Related Pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352

Bultreys

Gheysen

2000

Appl Environ Microbiol

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“…Data from biological and analytical tests, such as pyoverdine-mediated bacterial-growth stimulation, pyoverdine-mediated iron uptake, pyoverdine isoelectric focusing (IEF) analysis and immunodetection of the outer-membrane ferripyoverdine receptors, allowed the recognition of three different pyoverdine systems among these strains. This multiplicity correlated well with the three P. aeruginosa pyoverdine structures obtained by chemical analysis (Briskot et al, 1989; Gipp et al, 1991; Tappe et al, 1993; Meyer et al, 1997). Thus, by the use of a limited panel of easily and rapidly performed assays which have been defined as ‘siderotyping’ (Meyer et al, 1997), it was possible to classify all P. aeruginosa strains into one of three groups or ‘siderovars’, each siderovar consisting of strains producing the same pyoverdine, as evident from IEF, and having the same specific pyover- dine-mediated iron-uptake system, as judged by growth stimulation, iron uptake and ferripyoverdine receptor properties.…”

Section: Introductionsupporting

confidence: 77%

“…Siderophore-based methods for distinguishing fluorescent Pseudomonas strains through their siderophore (pyoverdine)-mediated iron-uptake system were initially investigated with a small number of Pseudomonas aeruginosa strains (Cornelis et al, 1989), and recently extended to a larger set of about 100 collection and clinical isolates of the same species (Meyer et al, 1997). Data from biological and analytical tests, such as pyoverdine-mediated bacterial-growth stimulation, pyoverdine-mediated iron uptake, pyoverdine isoelectric focusing (IEF) analysis and immunodetection of the outer-membrane ferripyoverdine receptors, allowed the recognition of three different pyoverdine systems among these strains.…”

Section: Introductionmentioning

confidence: 99%

Siderotyping of fluorescent pseudomonads:characterization of pyoverdines of Pseudornonas fluorescens and Pseudornonas putida strains from Antarctica

et al. 1998

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Siderotyping of fluorescent pseudomonads t characterization of pyoverdines of Pseudornonas fluorescens and Pseudornonas putida strains from Antarctica J ea n-M a r ie M eyer, Al a in S t i ntz i, Va I& i e Cou I a ng es, S isi nt h y S h ivaj i * Jessica A. VossJ3 Kambiz Taraz3 and Herbert Budzikiewicz3 Five independent fluorescent pseudomonad isolates originating from Antarctica were analysed for their pyoverdine systems. A pyoverdine-related siderotyping, which involved pyoverdine-induced growth stimulation, pyoverdine-mediated iron uptake, pyoverdine analysis by electrophoresis and isoelectric focusing, revealed three different pyoverdine-related siderotypes among the five isolates. One siderotype, including Pseudomonas fluorescens 1W and P. fluorescens lOcW, was identical to that of P. fluorescens ATCC 13525. Two other strains, P. fluorescens 9AW and Pseudomonas putida 9BW, showed identical pyoverdine-related behaviour t o each other, whereas the fifth strain, P. fluorescens 5lW, had unique features compared to the other strains or t o a set of 12 fluorescent Pseudomonas strains used as comparison material. Elucidation of the structure of the pyoverdines produced by the Antarctic strains supported the accuracy of the siderotyping methodology by confirming that pyoverdines from strains 1W and 1OcW had the same structures as the P. fluorescens ATCC 13525 pyoverdine, whereas the 9AW and 9BW pyoverdines are probably identical with the pyoverdine of P. fluorescens strain 244. Pyoverdine from strain 51W appeared t o be a novel pyoverdine since i t s structure was different from all previously established pyoverdine structures. Together with the conclusion that the Antarctic Pseudomonas strains have no special features at the level of their pyoverdines and pyoverdine-mediated iron metabolism compared to worldwide strains, the present work demonstrates that siderotyping provides a rapid means of screening for novel pyoverdines.

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“…S1) (5). β-OHHis has since been identified in the peptide of pyoverdines from a variety of pseudomonads (6)(7)(8). Some Pseudomonas strains produce the fatty-acyl peptidic siderophores corrugatin, ornicorrugatin, or histicorrugatin (SI Appendix, Fig.…”

mentioning

confidence: 99%

Genomic analysis of siderophore β-hydroxylases reveals divergent stereocontrol and expands the condensation domain family

Reitz

Hardy

Suk

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Genome mining of biosynthetic pathways streamlines discovery of secondary metabolites but can leave ambiguities in the predicted structures, which must be rectified experimentally. Through coupling the reactivity predicted by biosynthetic gene clusters with verified structures, the origin of the β-hydroxyaspartic acid diastereomers in siderophores is reported herein. Two functional subtypes of nonheme Fe(II)/α-ketoglutarate–dependent aspartyl β-hydroxylases are identified in siderophore biosynthetic gene clusters, which differ in genomic organization—existing either as fused domains (IβHAsp) at the carboxyl terminus of a nonribosomal peptide synthetase (NRPS) or as stand-alone enzymes (TβHAsp)—and each directs opposite stereoselectivity of Asp β-hydroxylation. The predictive power of this subtype delineation is confirmed by the stereochemical characterization of β-OHAsp residues in pyoverdine GB-1, delftibactin, histicorrugatin, and cupriachelin. The l-threo (2S, 3S) β-OHAsp residues of alterobactin arise from hydroxylation by the β-hydroxylase domain integrated into NRPS AltH, while l-erythro (2S, 3R) β-OHAsp in delftibactin arises from the stand-alone β-hydroxylase DelD. Cupriachelin contains both l-threo and l-erythro β-OHAsp, consistent with the presence of both types of β-hydroxylases in the biosynthetic gene cluster. A third subtype of nonheme Fe(II)/α-ketoglutarate–dependent enzymes (IβHHis) hydroxylates histidyl residues with l-threo stereospecificity. A previously undescribed, noncanonical member of the NRPS condensation domain superfamily is identified, named the interface domain, which is proposed to position the β-hydroxylase and the NRPS-bound amino acid prior to hydroxylation. Through mapping characterized β-OHAsp diastereomers to the phylogenetic tree of siderophore β-hydroxylases, methods to predict β-OHAsp stereochemistry in silico are realized.

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Identical Pyoverdines from Pseudomonas fluorescens 9AW and from Pseudomonas putida 9BW

Cited by 21 publications

References 0 publications

Production and Comparison of Peptide Siderophores from Strains of Distantly Related Pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352

Production and Comparison of Peptide Siderophores from Strains of Distantly Related Pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352

Siderotyping of fluorescent pseudomonads:characterization of pyoverdines of Pseudornonas fluorescens and Pseudornonas putida strains from Antarctica

Genomic analysis of siderophore β-hydroxylases reveals divergent stereocontrol and expands the condensation domain family

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