Idazoxan activates rat forebrain glycogen phosphorylase in vivo: A histochemical study

Fara-On, Maria; Evans, John H.; Harley, Carolyn W.

doi:10.1016/j.brainres.2005.08.028

Cited by 2 publications

(1 citation statement)

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“…GP measurements in saline controls were similar to those reported previously in the hippocampus (Harley & Bielajew, 1992; Uecker et al ., 1997; Fara‐On et al ., 2005) and entorhinal cortex (Walling et al ., 2006). Changes in GP one hour after status in the present study also were similar to previous studies assessing GP activity using other convulsants, such as methionine sulphoximine.…”

Section: Discussionmentioning

confidence: 99%

Acute and chronic changes in glycogen phosphorylase in hippocampus and entorhinal cortex after status epilepticus in the adult male rat

Walling

Rigoulot

Scharfman

2007

Eur J of Neuroscience

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Glial cells provide energy substrates to neurons, in part from glycogen metabolism, which is influenced by glycogen phosphorylase (GP). To gain insight into the potential subfield and laminarspecific expression of GP, histochemistry can be used to evaluate active GP (GPa) or totalGP (GPa + GPb). Using this approach, we tested the hypothesis that changes in GP would occur under pathological conditions that are associated with increased energy demand, i.e. severe seizures (status epilepticus or 'status'). We also hypothesized that GP histochemistry would provide insight into changes in the days and weeks after status, particularly in the hippocampus and entorhinal cortex, where there are robust changes in structure and function. One hour after the onset of pilocarpineinduced status, GPa staining was reduced in most regions of the hippocampus and entorhinal cortex relative to saline-injected controls. One week after status, there was increased GPa and totalGP, especially in the inner molecular layer, where synaptic reorganization of granule cell mossy fibre axons occurs (mossy fibre sprouting). In addition, patches of dense GP reactivity were evident in many areas. One month after status, levels of GPa and totalGP remained elevated in some areas, suggesting an ongoing role of GP or other aspects of glycogen metabolism, possibly due to the evolution of intermittent, recurrent seizures at approximately 3-4 weeks after status. Taken together, the results suggest that GP is dynamically regulated during and after status in the adult rat, and may have an important role in the pilocarpine model of epilepsy.

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