This article investigates the relationship between policy (conceptualised as goals, values and resources), organisational culture and elearning use. Through both qualitative and quantitative research methods, we gathered data about staff and student perspectives from four diverse South African universities representing a selection of ICT in education policy types (Structured and Unstructured) and organisational cultural types of "collegium, bureaucracy, corporate and enterprise" (McNay 1995). While our findings show a clear relationship between policy and use of ICTs for teaching and learning, organisational culture is found crucial to policy mediation and the way that elearning use is embedded within the organisation. We conclude that although a Structured Corporate institutional type enables the attainment of a "critical mass" within e-learning, Unstructured Collegium institutions are better at fostering innovation. Unstructured Bureaucratic institutions are the least enabling of either top-down or bottom-up elearning change.