ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn.

Türkan, Fikret; Atalar, Mehmet Nuri; Aras, Abdülmelik; Gülçın, İlhami; Bursal, Ercan

doi:10.1016/j.bioorg.2019.103333

Cited by 78 publications

(26 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antioxidant properties exhibited by leaf extracts may be correlated with the presence of secondary metabolites in plants extracts. Our results match with previous reports [ 83 – 85 ].…”

Section: Resultssupporting

confidence: 94%

Phytochemical analysis and versatile in vitro evaluation of antimicrobial, cytotoxic and enzyme inhibition potential of different extracts of traditionally used Aquilegia pubiflora Wall. Ex Royle

Jan

Usman

Shah

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Himalayan Columbine (Aquilegia pubiflora Wall. Ex Royle) is a medicinal plant and have been used as traditional treatments for various human diseases including skin burns, jaundice, hepatitis, wound healing, cardiovascular and circulatory diseases. Till now there is no report available on phytochemical investigation of Himalayan Columbine and to the best of our knowledge, through present study we have reported for the first time, the phytochemical analysis and pharmacological potentials of different leaf extracts of Aquilegia pubiflora. Methods Four types of extracts were prepared using solvent of different polarities (Distilled water APDW, Methanol APM, Ethanol APE and Ethyl acetate APEA), and were evaluated to determine the best candidate for potent bioactivity. Phytochemical constituents in prepared extracts were quantified through HPLC analysis. Subsequently, all four types of leaf extracts were then evaluated for their potential bioactivities including antimicrobial, protein kinase inhibition, anti-inflammatory, anti-diabetic, antioxidant, anti-Alzheimer, anti-aging and cytotoxic effect. Results HPLC analysis demonstrated the presence of dvitexin, isovitexin, orientin, isoorientin, ferulic acid, sinapic acid and chlorogenic acid in varied proportions in all plant extracts. Antimicrobial studies showed that, K. pneumonia was found to be most susceptible to inhibition zones of 11.2 ± 0.47, 13.9 ± 0.33, 12.7 ± 0.41, and 13.5 ± 0.62 measured at 5 mg/mL for APDW, APM, APE and APEA respectively. A. niger was the most susceptible strain in case of APDW with the highest zone of inhibition 14.3 ± 0.32, 13.2 ± 0.41 in case of APM, 13.7 ± 0.39 for APE while 15.4 ± 0.43 zone of inhibition was recorded in case of APEA at 5 mg/mL. The highest antioxidant activity of 92.6 ± 1.8 μgAAE/mg, 89.2 ± 2.4 μgAAE/mg, 277.5 ± 2.9 μM, 289.9 ± 1.74 μM for TAC, TRP, ABTS and FRAP, respectively, was shown by APE. APM, APE and APEA extracts showed a significant % cell inhibition (above 40%) against HepG2 cells. The highest anti-inflammatory of the samples was shown by APE (52.5 ± 1.1) against sPLA2, (41.2 ± 0.8) against 15-LOX, followed by (38.5 ± 1.5) and (32.4 ± 0.8) against COX-1 and COX-2, respectively. Conclusions Strong antimicrobial, Protein Kinase potency and considerable α-glucosidase, α-amylase, and cytotoxic potential were exhibited by plant samples. Significant anti-Alzheimer, anti-inflammatory, anti-aging, and kinase inhibitory potential of each plant sample thus aware us for further detailed research to determine novel drugs.

show abstract

Section: Resultssupporting

confidence: 94%

Phytochemical analysis and versatile in vitro evaluation of antimicrobial, cytotoxic and enzyme inhibition potential of different extracts of traditionally used Aquilegia pubiflora Wall. Ex Royle

Jan

Usman

Shah

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…The results clearly showed that reducing power of EEFO and WEFO and standards decreased in the following order (Table 1): Ascorbic acid (λ700: 1.624±0.015, r 2 : 0.9930) > BHT (λ700: 0.909±0.006, r 2 : 0.9874) > α-tocopherol (λ700: 0.755±0.075, r 2 : 0.9867) > EEFO (λ700: 0.604±0.004, r 2 : 0.9843) and WEFO (λ700: 0.358±0.016, r 2 : 0.9373). In this assay, ferric ions (Fe 3+ ) are reduced to ferrous ions (Fe 2+ ) in the presence of an antioxidant molecule or extract at the acidic medium (pH 3.6) [65]. Copper is one of putative metals, which can occur in nature in a directly usable metallic form.…”

Section: Resultsmentioning

confidence: 99%

Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

Kızıltaş¹,

Gören²,

Bingöl³

et al. 2021

Rec.Nat.Prod.

Self Cite

View full text Add to dashboard Cite

To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.

show abstract

“…Type 2 diabetes can be managed through prescribed medication such as hydrolysing carbohydrates inhibitors (Acarbose and miglitol), which decreases the exaggerated increase in the glucose level that follows a meal (post prandial hyperglycaemia) ( Nebih et al., 2020 ; Gulcin et al., 2020 ). This is achieved by slowing down the rate in which glucose is absorbed into the blood system blocking the enzymes needed for hydrolysing complex carbohydrates (starch) to absorbable monosaccharides ( Turkan et al., 2020 ). Alpha amylase located in the saliva and in the pancreatic juice, helps in the digestion of polysaccharides into glucose and maltose.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves

Mogole

Omwoyo

Mtunzi

2020

Heliyon

View full text Add to dashboard Cite

Diabetes Mellitus is an endocrine disorder which causes insulin deficiency. Medicinal plants are documented to be efficacious in the management of the disease. The current research set to determine the phytochemicals present, anti-oxidant activity and investigate the potency of Eriobotrya japonica against α-amylase inhibition. The leaves of the plant were extracted sequentially. The different extracts were evaluated for the presence of phytochemicals and their potential anti-oxidant and α-amylase inhibition activity. Methanol with the highest polarity, gave the highest yield of 20% and hexane with the lowest polarity have the lowest yield of 2.09%. This trend resembled that observed for the total flavanoid and total phenolic content analysis which gave values of 0.3822 mg QAE/mg and 3.810 mg GAE/mg respectively for methanolic and hexane extracts. The extracts of methanol recorded higher DPPH free radical scavenging activity of 87% and gave the lowest IC 50 of 0.5336 which was below that of ascorbic acid used as a control. Hexane extract had a higher α-amylase inhibitory activity of 24% at 1 μg/ml as compared to other extracts. Generally hexane extracts of Eriobotrya japonica exhibits mild inhibitory activity against α-amylase enzyme which is recommended than the conventional therapy which maximally inhibits the enzyme causing major side effects. The results obtained herein support the use of the plant as an anti-diabetic agent at higher concentrations.

show abstract

ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn.

Cited by 78 publications

References 40 publications

Phytochemical analysis and versatile in vitro evaluation of antimicrobial, cytotoxic and enzyme inhibition potential of different extracts of traditionally used Aquilegia pubiflora Wall. Ex Royle

Phytochemical analysis and versatile in vitro evaluation of antimicrobial, cytotoxic and enzyme inhibition potential of different extracts of traditionally used Aquilegia pubiflora Wall. Ex Royle

Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

Phytochemical screening, anti-oxidant activity and α-amylase inhibition study using different extracts of loquat (Eriobotrya japonica) leaves

Contact Info

Product

Resources

About