“…This limitation was possibly due to incomplete reference data of local species in genomic databases, which has also been reported for other ecosystems within the Neotropics (Cilleros et al., 2019; Milan et al., 2020; de Santana et al., 2021; Jackman et al., 2021; Mariac et al., 2021; Sales et al., 2021; Lozano Mojica & Caballero, 2021; Batista et al., 2021; Cantera et al., 2022; Mariac et al., 2022; Coutant et al., 2023), especially for the 12S marker used in this study (Cilleros et al., 2019; de Santana et al., 2021; Jackman et al., 2021). We selected the 12S marker because it was previously reported to be used to assess fish diversity with success, not only in sea and deepwater environments (Miya et al., 2015; Riaz et al., 2011; Thomsen et al., 2016; Valentini et al., 2016), but also in freshwater from neotropical systems (Jackman et al., 2021; Milan et al., 2020; Sales et al., 2021; Teixeira et al., 2021) and the tropics in general (Blackman et al., 2021). A highly specific and universal marker, such as the 12S‐rRNA region targeted in this study, may be suitable for a scarcely explored environment such as the Amazon basin since the coverage to detect unknown species might be improved (Collins et al., 2019).…”