Iceberg Model of the Structure of Globular Protein Adsorption Layers at the Air–Water Interface. Study by the Tritium Planigraphy Technique

Bogacheva, E. N.; Gedrovich, A. V.; Shishkov, A.V.

doi:10.1023/b:coll.0000023112.02071.e3

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…This anomaly may be reconciled by considering that the lysozyme extends above the air-water interface, as reported recently. 1,44 In the proposed orientation, a movement of 3 Å would only expose proline 70 and glycine 71 to air. The modified X-ray SLD profile allowing for 3 Å exposure (two slabs exposed) also is shown in Figure 4.…”

Section: Modeling the Orientation Of Lysozymementioning

confidence: 99%

Effect of the Air−Water Interface on the Structure of Lysozyme in the Presence of Guanidinium Chloride

et al. 2008

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We report observations of the changes in the surface structure of lysozyme adsorbed at the air-water interface produced by the chemical denaturant guanidinium chloride. A primary result is the durability of the adsorbed surface layer to denaturation, as compared to the molecule in the bulk solution. Data on the surface film were obtained from X-ray and neutron reflectivity measurements and modeled simultaneously. The behavior of lysozyme in G.HCl solutions was determined by small-angle X-ray scattering. For the air-water interface, determination of the adsorbed protein layer dimensions shows that at low to moderate denaturant concentrations (up to 2 mol L(-1)), there is no significant distortion of the protein's tertiary structure at the interface, as changes in the orientation of the protein are sufficient to model data. At higher denaturant concentrations, time-dependent multilayer formation occurred, indicating molecular aggregation at the surface. Methodologies to predict the protein orientation at the interface, based on amino acid residues' surface affinities and charge, were critiqued and validated against our experimental data.

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Section: Modeling the Orientation Of Lysozymementioning

confidence: 99%

Effect of the Air−Water Interface on the Structure of Lysozyme in the Presence of Guanidinium Chloride

et al. 2008

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“…The solid-state chemistry of water clusters continues to attract considerable attention. [1][2][3][4][5][6][7][8][9][10] It seems clear that trapping small, finite "icicles" within a crystalline host matrix offers considerable scope for isolating and studying their properties, some of which may be of relevance to biological systems [11][12][13][14][15][16][17] or clathrate hydrate research. [18][19][20] The observation of small water aggregates of a variety of structural types within organic or metal-organic crystal structures is commonplace.…”

Section: Introductionmentioning

confidence: 99%

“…The solid-state chemistry of water clusters continues to attract considerable attention. − It seems clear that trapping small, finite “icicles” within a crystalline host matrix offers considerable scope for isolating and studying their properties, some of which may be of relevance to biological systems − or clathrate hydrate research. − The observation of small water aggregates of a variety of structural types within organic or metal−organic crystal structures is commonplace. For example, a search of the Cambridge Structural Database (CSD) for structures containing at least three ordered water molecules, with H atoms located and O−H bond distances normalized to typical neutron values, mutually linked with H···O contacts within the sum of the van der Waals radii, reveals a total of 2267 hits.…”

Section: Introductionmentioning

confidence: 99%

Cooperative Hydrogen-Bonding Effects in a Water Square: A Single-Crystal Neutron and Partial Atomic Charges and Hardness Analysis Study

et al. 2005

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Four isomorphous complexes of formula [M(L)(4)(H(2)O)(2)]SO(4).2H(2)O (M = Co, 1a; Ni, 1b; Cu, 1c; Zn, 1d) have been isolated and characterized by single-crystal X-ray diffraction and neutron diffraction using the quasi-Laue diffractometer VIVALDI at the Institut Laue-Langevin as well as by thermogravimetric analysis. The structures contain a discrete, strongly hydrogen-bonded water tetramer which causes a significant distortion of the metal coordination sphere in each case. Partial atomic charges and hardness analysis (PACHA) calculations reveal that the shortest hydrogen bonds are not the strongest in this constrained, cyclic solid-state structure and show that the distortion at the metal center is caused by the drive to maintain the integrity of the water tetramer. The system undergoes a disorder-order transition on slow cooling that provides insight into the nature of communication between water squares.

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Exploring proteins at soft interfaces and in thin liquid films – From classical methods to advanced applications of reflectometry

Gochev,

Campbell,

Schneck

et al. 2024

Advances in Colloid and Interface Science

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Iceberg Model of the Structure of Globular Protein Adsorption Layers at the Air–Water Interface. Study by the Tritium Planigraphy Technique

Cited by 4 publications

References 11 publications

Effect of the Air−Water Interface on the Structure of Lysozyme in the Presence of Guanidinium Chloride

Effect of the Air−Water Interface on the Structure of Lysozyme in the Presence of Guanidinium Chloride

Cooperative Hydrogen-Bonding Effects in a Water Square: A Single-Crystal Neutron and Partial Atomic Charges and Hardness Analysis Study

Exploring proteins at soft interfaces and in thin liquid films – From classical methods to advanced applications of reflectometry

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