ICE
 Sp1116
 , the Genetic Element Responsible for
 erm
 (B)-Mediated, Inducible Erythromycin Resistance in Streptococcus pyogenes, Belongs to the Tn
 GBS
 Family of Integrative and Conjugative Elements

Brenciani, Andrea; Tiberi, Erika; Morroni, Gianluca; Mingoia, Marina; Varaldo, Pietro E.; Giovanetti, Eleonora

doi:10.1128/aac.00048-14

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…gallolyticus strain DSM 16831 was increased after adhesion to collagen. One region consists of transposon genes, and has similarities to ICE Sp 1116 of Streptococcus pyogenes [ 60 , 61 ]. The phage genes whose expression was upregulated through collagen adhesion (the second region) have similarities to the streptococcal bacteriophage P9 [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

et al. 2018

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Streptococcus gallolyticus subsp. gallolyticus is a commensal bacterium of the human gastrointestinal tract, and a pathogen causing infective endocarditis and other biofilm-associated infections via exposed collagen. This study focuses on the characterization of the biofilm formation and collagen adhesion of S. gallolyticus subsp. gallolyticus under different conditions. In this study, it has been observed that the isolate UCN 34 is resistant to 20 mg/ ml lysozyme in BHI medium, whereas the strain BAA-2069 builds more biofilm in the presence of lysozyme compared to in a control of BHI without lysozyme. A transcriptome analysis with whole genome microarrays of these two isolates in BHI medium with lysozyme compared to control without lysozyme revealed changes in gene expression levels. In the isolate BAA-2069, 67 genes showed increased expression in the presence of lysozyme, while in the isolate UCN 34, 165 genes showed increased expression and 30 genes showed decreased expression through lysozyme treatment. Products of genes which were higher expressed are in involved in transcription and translation, in cell-wall modification, in hydrogen peroxide resistance and in bacterial immunity. Furthermore, the adhesion ability of different strains of S. gallolyticus subsp. gallolyticus to collagen type I and IV was analyzed. Thereby, we compared the adhesion of 46 human isolates with 23 isolates from animals. It was shown that the adhesion ability depends significantly on whether the isolate was isolated from human or animal. For example, high adhesion ability was observed for strain UCN 34 isolated from an infective endocarditis patient, whereas strain DSM 16831 isolated from koala feces adhered only marginally to collagen. Full genome microarray analysis of these two strains revealed strain-dependent gene expression due to adhesion. The expression of 25 genes of a transposon and 15 genes of a phage region in strain DSM 16831 were increased, which corresponds to horizontal gene transfer. Adherence to collagen in strain UCN 34 led to higher expression of 27 genes and lower expression of 31 genes. This was suggestive of a change in nutrient uptake.

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Section: Discussionmentioning

confidence: 99%

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

et al. 2018

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“…The three strains being examined (S. pneumoniae Spn529, S. pyogenes Spy029, and S. pyogenes Spy005) were used as donors. Six rifampin-and fusidic acid-resistant (RF) derivatives of erythromycin-and chloramphenicol-susceptible strains of different Streptococcus species were used as recipients: S. pneumoniae strain R6RF (2), S. pyogenes strain 12RF (18), Streptococcus agalactiae strain 1357RF (19), Streptococcus oralis strain 1235RF and Streptococcus gordonii strain 1435RF (20), and Streptococcus dysgalactiae subsp. equisimilis strain 5381RF, an RF derivative obtained for this study from a recently investigated strain (21).…”

Section: Methodsmentioning

confidence: 99%

Tn 5253 Family Integrative and Conjugative Elements Carrying mef (I) and catQ Determinants in Streptococcus pneumoniae and Streptococcus pyogenes

Mingoia

Morici

Morroni

et al. 2014

Antimicrob Agents Chemother

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The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the int Sp23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int 5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (int Sp23FST81 or int 5252 ). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029.

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Global genomic epidemiology of Streptococcus pyogenes

Jespersen

Lacey

Tong

et al. 2020

Infection, Genetics and Evolution

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ICE Sp1116 , the Genetic Element Responsible for erm (B)-Mediated, Inducible Erythromycin Resistance in Streptococcus pyogenes, Belongs to the Tn GBS Family of Integrative and Conjugative Elements

Cited by 5 publications

References 13 publications

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

Tn 5253 Family Integrative and Conjugative Elements Carrying mef (I) and catQ Determinants in Streptococcus pneumoniae and Streptococcus pyogenes

Global genomic epidemiology of Streptococcus pyogenes

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