ICE<i>Ec2</i>, a New Integrative and Conjugative Element Belonging to the pKLC102/PAGI-2 Family, Identified in<i>Escherichia coli</i>Strain BEN374

Roche, David; Fléchard, Maud; Lallier, Nathalie; Répérant, Maryline; Brée, Annie; Pascal, Géraldine; Schouler, Catherine; Germon, Pierre

doi:10.1128/jb.00609-10

Cited by 22 publications

(24 citation statements)

References 54 publications

(74 reference statements)

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“…1). Thus, the integrase in conjunction with the adjacent gene appears to be responsible for ICE excision, concurring with previous data from elements ICEEc2 and PAPI-1 (57,60).…”

Section: Resultssupporting

confidence: 80%

“…The ability of the ICEs to excise and circularize was assessed as described above. The novel Salmonelladerived elements ICESe3, ICESe4, and CTnscr94 were found to be capable of excision, as were HAI2 from E. carotovora and ICEEc2 from E. coli (60), but no amplicons indicative of excision or circularization were obtained for the element within C. rodentium strain ICC168 or either of the elements from Y. enterocolitica, indicating that these latter three are unable to excise under the selected experimental conditions (Table 3).…”

Section: Resultsmentioning

confidence: 99%

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Structure, Diversity, and Mobility of the Salmonella Pathogenicity Island 7 Family of Integrative and Conjugative Elements within Enterobacteriaceae

et al. 2012

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Integrative and conjugative elements (ICEs) are self-mobile genetic elements found in the genomes of some bacteria. These elements may confer a fitness advantage upon their host bacteria through the cargo genes that they carry. Salmonella pathogenicity island 7 (SPI-7), found within some pathogenic strains of Salmonella enterica, possesses features indicative of an ICE and carries genes implicated in virulence. We aimed to identify and fully analyze ICEs related to SPI-7 within the genus Salmonella and other Enterobacteriaceae. We report the sequence of two novel SPI-7-like elements, found within strains of Salmonella bongori, which share 97% nucleotide identity over conserved regions with SPI-7 and with each other. Although SPI-7 within Salmonella enterica serovar Typhi appears to be fixed within the chromosome, we present evidence that these novel elements are capable of excision and self-mobility. Phylogenetic analyses show that these Salmonella mobile elements share an ancestor which existed approximately 3.6 to 15.8 million years ago. Additionally, we identified more distantly related ICEs, with distinct cargo regions, within other strains of Salmonella as well as within Citrobacter, Erwinia, Escherichia, Photorhabdus, and Yersinia species. In total, we report on a collection of 17 SPI-7 related ICEs within enterobacterial species, of which six are novel. Using comparative and mutational studies, we have defined a core of 27 genes essential for conjugation. We present a growing family of SPI-7-related ICEs whose mobility, abundance, and cargo variability indicate that these elements may have had a large impact on the evolution of the Enterobacteriaceae. Bacterial genomes are often represented as a core of conserved genes interspersed with accessory regions which are variably present throughout members of a species. The ability of an organism to sample variation through accessing a pan-genome can be strongly associated with a change in lifestyle or adaptation to a new niche (29). In some cases, acquired accessory functions become fixed and go on to define a particular lineage (30). A classical example of this is the acquisition of the Salmonella pathogenicity islands (SPIs), considered to have been fundamental to the evolution of the genus Salmonella (26). The largest of these islands identified to date is SPI-7, found within the genomes of Salmonella enterica subsp. enterica serovar Typhi (S. Typhi) (52), Salmonella enterica subsp. enterica serovar Paratyphi C (S. Paratyphi C) (43), and some strains of Salmonella enterica subsp. enterica serovar Dublin (S. Dublin) (55). SPI-7 is 120 kb in length and encodes important virulence functions, including the major virulence antigen (Vi) and type IVB pili (24, 52). Additionally, integrated within SPI-7 from strains of S. Typhi is a prophage harboring the type 3 secretion system effector protein-encoding gene sopE (47).SPI-7 possesses many similarities to integrative and conjugative elements (ICEs), a class of self-transmissible elements (7) also known as conjugativ...

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“…1). Thus, the integrase in conjunction with the adjacent gene appears to be responsible for ICE excision, concurring with previous data from elements ICEEc2 and PAPI-1 (57,60).…”

Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Structure, Diversity, and Mobility of the Salmonella Pathogenicity Island 7 Family of Integrative and Conjugative Elements within Enterobacteriaceae

et al. 2012

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“…Some ICEs such as ICE Ec2 from E. coli carry a Tn 7 ‐related transposon. The Tn 7 found in ICE Ec2 carries an integron encoding resistance to trimethoprim, streptothricin, and streptomycin (Roche et al ., ).…”

Section: Evolution Of Conjugative and Mobilizable Geis And Consequencmentioning

confidence: 97%

Conjugative and mobilizable genomic islands in bacteria: evolution and diversity

et al. 2014

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Horizontal transfer of genomic islands (GEIs), that is, chromosomal regions encoding functions that can be advantageous for the host, plays a key role in bacterial evolution, but their mechanisms of transfer remained elusive for a long time. Recent data suggest that numerous GEIs belong to noncanonical classes of mobile genetic elements (MGEs) that can transfer by conjugation. Among them, the integrative and conjugative elements encode their own excision, conjugative transfer, and integration, whereas the integrative mobilizable elements are autonomous for excision and integration but require the conjugation machinery of helper elements to transfer. Others can self-transfer but require the recombination machinery of the recipient cell to integrate. All these MGEs evolve by acquisition, deletion, or exchange of modules, that is, groups of genes involved in the same function. Moreover, composite GEIs can result from the insertion of a MGE within another or from the site-specific integration of an incoming MGE into one of the recombination sites flanking a resident GEI (tandem accretion). Tandem accretion enables the cis-conjugative mobilization of highly degenerated and nonautonomous GEIs, the cis-mobilizable elements. All these mechanisms contribute to the plasticity and complex evolution of GEIs and explain the highly diverse tableau revealed by more and more genome comparisons.

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“…Several ICEs, including ICE Hin1056 (80), SXT-R391 (17, 73), and ICE Ec2 (126), carry insertion sequences and/or transposons. Other ICEs, including SXT ET (73), ICE Ec2 (126), and Tn 5253 (6, 77), contain integrons (reviewed in 142). In some cases, a plasmid has integrated, apparently by single crossover, into an ICE, for example in the case of Tn 5253 (6, 77).…”

Section: Cargo Genes and The Discovery Of Icesmentioning

confidence: 99%

Integrative and Conjugative Elements (ICEs): What They Do and How They Work

Johnson

Grossman²

2015

Annu. Rev. Genet.

436

459

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Horizontal gene transfer plays a major role in microbial evolution, allowing microbes to acquire new genes and phenotypes. Integrative and conjugative elements (ICEs, a.k.a. conjugative transposons) are modular mobile genetic elements integrated into a host genome and are passively propagated during chromosomal replication and cell division. Induction of ICE gene expression leads to excision, production of the conserved conjugation machinery (a type IV secretion system), and the potential to transfer DNA to appropriate recipients. ICEs typically contain cargo genes that are not usually related to the ICE life cycle and that confer phenotypes to host cells. We summarize the life cycle and discovery of ICEs, some of the regulatory mechanisms, and how the types of cargo have influenced our view of ICEs. We discuss how ICEs can acquire new cargo genes and describe challenges to the field and various perspectives on ICE biology.

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ICEEc2, a New Integrative and Conjugative Element Belonging to the pKLC102/PAGI-2 Family, Identified inEscherichia coliStrain BEN374

Cited by 22 publications

References 54 publications

Structure, Diversity, and Mobility of the Salmonella Pathogenicity Island 7 Family of Integrative and Conjugative Elements within Enterobacteriaceae

Structure, Diversity, and Mobility of the Salmonella Pathogenicity Island 7 Family of Integrative and Conjugative Elements within Enterobacteriaceae

Conjugative and mobilizable genomic islands in bacteria: evolution and diversity

Integrative and Conjugative Elements (ICEs): What They Do and How They Work

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