Icariin, the main prenylflavonoid of Epimedii Folium, ameliorated chronic kidney disease by modulating energy metabolism via AMPK activation

Zhao, Yongdan; Yang, Wanyue; Zhang, Xin; Lv, Chongning; Lu, Jincai

doi:10.1016/j.jep.2023.116543

Cited by 3 publications

(2 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional Chinese herbal medicine has been highly valued for a long time because of its abundant sources, low price, stable curative effect and few side effects (Zhao et al., 2023). However, the crude extracts of Chinese herbal medicines are complicated, and a fine exploration is needed to clarify the mechanism of Chinese herbal medicines to better promote their clinical application.…”

Section: Discussionmentioning

confidence: 99%

Icariin alleviates the apoptosis of chondrocytes in osteoarthritis through regulating SIRT‐1‐Nrf2‐HO‐1 signaling

Liu,

Zhong,

Liu

et al. 2024

Chem Biol Drug Des

View full text Add to dashboard Cite

Icariin has shown the potential to treat osteoarthritis (OA), but the specific mechanism still needs further exploration. Therefore, this study attempted to reveal the effect and mechanism of icariin on OA based on in vitro and in vivo experiments. In vivo, a mouse model of OA was established by cutting the anterior cruciate ligament, and 10 mg/kg icariin was given to mice orally. Then, the OA injury and pathological changes of cartilage tissue in mice were identified by OA index and hematoxylin and eosin staining. In vitro, the viability of C28/I2 cells incubated with different concentrations of icariin was detected by 3‐(4,5)‐dimethylthiahiazo (‐z‐y1)‐3,5‐di‐ phenytetrazoliumromide assay. Subsequently, C28/I2 cells induced by IL‐1β were used as the cell model of OA, the expression of Sirtuin (SIRT)‐1 in cells was knocked down, and icariin was added for intervention. Next, western blot was used to observe the expression level of sirtuin 1 (SIRT‐1)‐Nrf2‐heme oxygenase 1 (HO‐1) signaling pathway‐related proteins in cells of each group. Besides, cell viability and apoptosis were detected by MTT and apoptosis assay, and DNA damage was observed by comet assay. In vivo experiments, intragastric administration of icariin could effectively reduce the OA index of mice, improve the pathological changes of cartilage tissue, and obviously activated the SIRT‐1‐Nrf2‐HO‐1 signaling pathway. In vitro experiments, icariin did not exhibit toxic effect on C28/I2 cells, but could activate the SIRT‐1‐Nrf2‐HO‐1 signaling pathway, improve the viability, reduce the level of apoptosis and relieve the DNA damage in OA cells; however, these effects were inhibited by si‐ SIRT‐1. Icariin can improve the symptoms of OA by activating the SIRT‐1‐Nrf2‐HO‐1 signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Icariin alleviates the apoptosis of chondrocytes in osteoarthritis through regulating SIRT‐1‐Nrf2‐HO‐1 signaling

Liu,

Zhong,

Liu

et al. 2024

Chem Biol Drug Des

View full text Add to dashboard Cite

show abstract

“…Therefore, seeking a natural medicinal component to mitigate cisplatin-induced renal cell damage is of utmost importance. Icariin (ICA), the primary bioactive compound derived from the medicinal herb Epimedium, has been extensively utilized in the therapeutic management of systemic disorders including lupus nephritis, inflammatory bowel disease, rheumatoid arthritis, and cancer ( 5 , 6 ). Research by Xie et al ( 7 ) has demonstrated that ICA exhibits a potential to mitigate mortality and acute kidney injury in mice with sepsis induced by cecal ligation and puncture.…”

Section: Introductionmentioning

confidence: 99%

Exploration of the protective mechanisms of Icariin against cisplatin-induced renal cell damage in canines

Liu,

Xie,

Zhai

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

This study delves into the protective mechanisms of Icariin (ICA) against cisplatin-induced damage in Madin-Darby canine kidney (MDCK) cells. Comprising two distinct phases, the investigation initially employed a single-factor randomized design to ascertain the minimal cisplatin concentration eliciting MDCK cell damage, spanning concentrations from 0 to 16 mmol/L. Concurrently, various concentrations of ICA (ranging from 5 to 50 mmol/L) were combined with 1 mmol/L cisplatin to determine the most efficacious treatment concentration. Subsequent investigations utilized four treatment groups: control, 1 mmol/L cisplatin, 1 mmol/L cisplatin + 20 mmol/L ICA, and 1 mmol/L cisplatin + 25 mmol/L ICA, aimed at elucidating ICA's protective mechanisms. Findings from the initial phase underscored a significant reduction in MDCK cell viability with 1 mmol/L cisplatin in comparison to the control (P < 0.01). Notably, the inclusion of 20 and 25 mmol/L ICA substantively ameliorated MDCK cell viability under 1 mmol/L cisplatin (P < 0.01). Moreover, cisplatin administration induced an elevation in inflammatory factors, malondialdehyde (MDA), reactive oxygen species (ROS), and Bax protein levels, while concurrently suppressing superoxide dismutase (SOD), catalase (CAT), and Bcl-2 expression (P < 0.01). Conversely, supplementation of 20 and 25 mmol/L ICA demonstrated a marked increase in mitochondrial membrane potential and levels of SOD, CAT, and Bcl-2 (P < 0.01). These interventions effectively attenuated inflammatory responses and suppressed Bax protein expression (P < 0.05), consequently mitigating cisplatin-induced apoptosis in MDCK cells (P < 0.01). In summary, these findings elucidate the role of ICA in impeding apoptosis in cisplatin-induced MDCK cells by regulating inflammatory responses, oxidative stress, and autophagic protein expression.

show abstract

Assessment for the influence of processing methods on chemical compositions and pharmacodynamics of Epimedii Folium and revealing the component transformation mechanism

Zhao,

Li,

Yang

et al. 2024

Industrial Crops and Products

View full text Add to dashboard Cite

Icariin, the main prenylflavonoid of Epimedii Folium, ameliorated chronic kidney disease by modulating energy metabolism via AMPK activation

Cited by 3 publications

References 45 publications

Icariin alleviates the apoptosis of chondrocytes in osteoarthritis through regulating SIRT‐1‐Nrf2‐HO‐1 signaling

Icariin alleviates the apoptosis of chondrocytes in osteoarthritis through regulating SIRT‐1‐Nrf2‐HO‐1 signaling

Exploration of the protective mechanisms of Icariin against cisplatin-induced renal cell damage in canines

Assessment for the influence of processing methods on chemical compositions and pharmacodynamics of Epimedii Folium and revealing the component transformation mechanism

Contact Info

Product

Resources

About