Icariin promotes proliferation and osteogenic differentiation of rat adipose-derived stem cells by activating the RhoA-TAZ signaling pathway

Ye, Yaping; Jing, Xingzhi; Li, Na; Wu, Yuze; Li, Bingbing

doi:10.1016/j.biopha.2017.01.075

Cited by 35 publications

(39 citation statements)

References 38 publications

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“…Horny goat weed, also known as Epimedii herba or Ying Yang Huo , is extensively used to treat various diseases, including heart disease, osteoporosis, and rheumatism [ 16 , 17 ]. Icariin (ICA), one of the pharmacologically dynamic ingredients extracted from horny goat weed, possesses various therapeutic abilities, including neuroprotective, cardioprotective, anti-inflammatory, and anticancer actions [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

et al. 2020

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Nanodiamonds (NDs) have been used as drug delivery vehicles due to their low toxicity and biocompatibility. Recently, it has been reported that NDs have also osteogenic differentiation capacity. However, their capacity using NDs alone is not enough. To significantly improve their osteogenic activity, we developed icariin (ICA)-functionalized NDs (ICA-NDs) and evaluated whether ICA-NDs enhance their in vitro osteogenic capacity. Unmodified NDs and ICA-NDs showed nanosized particles that were spherical in shape. The ICA-NDs achieved a prolonged ICA release for up to 4 weeks. The osteogenic capacities of NDs, ICA (10 μg)-NDs, and ICA (50 μg)-NDs were demonstrated by alkaline phosphatase (ALP) activity; calcium content; and mRNA gene levels of osteogenic-related markers, including ALP, runt-related transcript factor 2 (RUNX2), collagen type I alpha 1 (COL1A1), and osteopontin (OPN). In vitro cell studies revealed that ICA (50 μg)-ND-treated MC3T3-E1 cells greatly increased osteogenic markers, including ALP, calcium content, and mRNA gene levels of osteogenic-related markers, including ALP, RUNX2, COL1A1, and OPN compared to ICA (10 μg)-NDs or ND-treated cells. These our data suggest that ICA-NDs can promote osteogenic capacity.

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Section: Introductionmentioning

confidence: 99%

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

et al. 2020

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“…Wei et al found that ERα-Wnt/β-catenin signaling was another pathway involved in the icariin-stimulated osteogenic differentiation of MSCs [13]. The most recent study by Ye et al found that the icariin-induced MSC differentiation was also related to the transcriptional activation of TAZ signaling [10]. The various signaling pathways revealed by these studies indicates the complicated network underlying icariininduced osteogenic differentiation of MSCs.…”

Section: Icariin Induced Osteogenetic Differentiation From Mscs Via Pmentioning

confidence: 98%

“…Zhao et al found that in osteoblasts (in vitro) icariin-induced osteogenic differentiation was Runx2-dependent [1]. Recently, Ye et al showed that icariin promoted proliferation and osteogenic differentiation of adipose-derived MSCs through activation of the RhoA-TAZ signaling pathway [10]. Furthermore, it was suggested that the icariin-activated PI3K/Akt pathway may be correlated with TAZ-related pathway during MSC osteogenesis.…”

Section: Conflict Of Interestmentioning

confidence: 99%

“…Consequently, with the osteogenic counteracting effects of icariin on MSCs, inhibition of TAZ upregulation by siRNA did not significantly influence PI3K/ Akt activation in icariin-treated MSCs, but icariin-induced upregulation of Runx2 and osteogenic differentiation in MSCs was counteracted. It could be concluded from these findings that icariin treatment activated PI3K/Akt and osteoblasts by independent research groups [10,11]. The drug was determined clinically safe and approved for use in drug preparations [1,11,12].…”

Section: Introductionmentioning

confidence: 99%

“…The application of icariin to enhance osteogenic differentiation of MSCs was promising. Previously, several studies have explored the mechanisms of icariin-induced osteogenic differentiation [1,10,13]. In a recent report, a novel mechanism has been revealed in which PI3K/Akt and its downstream pathway played a crucial role in osteogenic differentiation of MSCs induced by icariin [11].…”

Section: Introductionmentioning

confidence: 99%

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New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt

et al. 2017

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Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA–TAZ axis

Zheng

et al. 2019

Cell Biology International

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Silver nanoparticles (AgNps) are well established antibacterial agents, which have been reported to promote osteogenesis of stem cells. Ras homolog gene family member A (RhoA) and Tafazzin (TAZ) have been recently shown to be involved in osteogenic differentiation. The present study aimed to evaluate the effects of AgNps on osteogenic differentiation of human periodontal ligament fibroblasts (HPDLFs), and investigate the underlying mechanisms of AgNps activity. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay was employed to determine the safe concentration of AgNps in HPDLFs. Using an alkaline phosphatase assay, Alizarin red S staining and detection of the expression of alkaline phosphatase (ALP), runt‐related transcription factor 2 (RUNX2), osteocalcin (OCN), osterix (OSX), and collagen‐I, we found that AgNps, at a concentration range of 25–100 μM, promoted osteogenic differentiation of HPDLFs in a dose‐dependent manner. We also found that 100 μM AgNps up‐regulated the active RhoA expression level. The results of ALP activity and expression of osteogenic markers showed that the effects of AgNps on osteogenic differentiation were abrogated by a RhoA pathway inhibitor, C3 reagent. Additionally, silencing of TAZ attenuated the AgNps‐induced osteogenic differentiation of HPDLFs, as shown by decreased ALP activity and down‐regulation of osteogenic markers. Interestingly, TAZ knockdown had a very small effect on the activity of RhoA, whereas C3 suppressed the expression of TAZ, indicating that TAZ was a downstream effector of RhoA. In conclusion, the present work demonstrated that AgNps promoted osteogenic differentiation of HPDLFs by activating the RhoA–TAZ axis.

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Icariin promotes proliferation and osteogenic differentiation of rat adipose-derived stem cells by activating the RhoA-TAZ signaling pathway

Cited by 35 publications

References 38 publications

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro

New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt

Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA–TAZ axis

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