Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner

Zhao, Jiyuan; Ohba, Shinsuke; Shinkai, Masashige; Chung, Ung‐il; Nagamune, Teruyuki

doi:10.1016/j.bbrc.2008.02.054

Cited by 156 publications

(117 citation statements)

References 22 publications

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“…On the contrary, icariin (a flavonol glycoside) induced the expression of BMP-4 in osteoblasts (73) . Coumarin derivatives increased phosphorylation of SMAD1/5/8, p38 and ERK in a time-dependent manner.…”

Section: A Trzeciakiewicz Et Al 74mentioning

confidence: 99%

“…On the other hand, JNK activity and downstream transcription driven by transcription factor AP-1 are downregulated via a non-genomic oestrogen receptor-mediated pathway (99,110) . In general, oestrogen acts on osteoblasts by modulating the activity of cytosolic kinases and subsequent post-transcriptional modification of transcription factors, (94) POB Resveratrol 0·1-10 mM 48 h BMP-2 " MC3T3-E1 MG-63 Lee et al (2008) (81) MC3T3-E1 Emodin 5-10 mM 48 h Activation of BMP-2 promoter " Tang et al (2008) (92) Rat POB Coumarin (bergapten, imperatorin) 0·3-10 mM (73) MC3T3-E1 Icariin 0·1 nM -10 mM 3 d BMP-4 " hFOB, fetal human osteoblastic cell line; " , increase; MG-63, human osteosarcoma cell line; SMAD, mothers against decapentaplegic homologue; ERK, extracellular signal-regulated kinase; POB, primary osteoblasts; MC3T3-E1, murine osteoblastic cells.…”

Section: What About Polyphenols and Oestrogen Receptor Signalling?mentioning

confidence: 99%

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When nutrition interacts with osteoblast function: molecular mechanisms of polyphenols

2009

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Recent research has provided insights into dietary components that may optimise bone health and stimulate bone formation. Fruit and vegetable intake, as well as grains and other plant-derived food, have been linked to decreased risk of major chronic diseases including osteoporosis. This effect has been partially attributed to the polyphenols found in these foods. Thus, it has been suggested that these compounds may provide desirable bone health benefits through an action on bone cell metabolism. The present review will focus on how some polyphenols can modulate osteoblast function and reports which cellular signalling pathways are potentially implicated. However, to date, despite numerous investigations, few studies have provided clear evidence that phenolic compounds can act on osteoblasts. Polyphenols cited in the present review seem to be able to modulate the expression of transcription factors such as runt-related transcription factor-2 (Runx2) and Osterix, NF-κB and activator protein-1 (AP-1). It appears that polyphenols may act on cellular signalling such as mitogen-activated protein kinase (MAPK), bone morphogenetic protein (BMP), oestrogen receptor and osteoprotegerin/receptor activator of NF-κB ligand (OPG/RANKL) and thus may affect osteoblast functions. However, it is also important to take in account the possible interaction of these compounds on osteoclast metabolism to better understand the positive correlation reported between the consumption of fruit and vegetables and bone mass.

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Section: A Trzeciakiewicz Et Al 74mentioning

confidence: 99%

Section: What About Polyphenols and Oestrogen Receptor Signalling?mentioning

confidence: 99%

When nutrition interacts with osteoblast function: molecular mechanisms of polyphenols

2009

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“…The in vitro bioactivity assay revealed that the loaded ICA was biologically active [22]. ICA increases bone marrow cells differentiation and bone mineralization most likely by upregulating the expression of NO synthesis, subsequently regulating Cbfa1/Runx2 [23,24]. Meanwhile, ICA can inhibit bone resorption by reducing osteoclastic differentiation and induce osteoclasts apoptosis through an MAPKs/NF-B mechanism [25].…”

Section: Introductionmentioning

confidence: 99%

Icariin-Loaded TiO₂ Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells

Liu

Chen

et al. 2018

Journal of Nanomaterials

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To explore the effects of icariin on the biocompatibility of dental implants, icariin-(ICA-) loaded TiO 2 nanotubes were fabricated on Ti substrates via anodic oxidation and physical absorption. The surface characteristics of the specimens were monitored by field emission scanning electron microscopy (FE-SEM), X-ray diffractometry (XRD), contact angle measurements (CA), and highpressure liquid chromatography. Additionally, the activities of bone marrow cells, such as cytoskeletal, proliferative activities, mineralization, and osteogenesis-related gene expression on the substrates were investigated in detail. The characterization results demonstrated that ICA-loaded TiO 2 nanotubes were successfully fabricated and the hydrophilicity of these TiO 2 nanotubes was significantly higher than that of the pure Ti groups. The results also showed that ICA-loaded TiO 2 nanotubes might not have enhanced effects on cell proliferation and ALP expression. However, it seemed to significantly promote differentiation of bone marrow cells, demonstrated by enhancing the formation of mineralized nodule and the upregulation of the gene expression such as OC, BSP, OPN, and COL-1. The results indicated that ICA-loaded TiO 2 nanotubes can modulate bioactivity of bone marrow cells, which is promising for potential applications in the orthopedics field.

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“…Meanwhile, severe bone disorders are also increasingly prevalent with the greying of society [1,2]. For serious bone defects, it is usually difficult to heal through endogenous regenerative mechanisms, especially for aged patients.…”

Section: Introductionmentioning

confidence: 99%

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

et al. 2015

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Collagen hydrogels were considered as favourable scaffolding for tissue engineering. It was demonstrated that cytokines and siRNAs could be efficiently retained by collagen hydrogels for controlled release thereby enhancing their bioactivities. Basic fibroblast growth factor (bFGF) was a stimulator for osteogenic differentiation of mesenchymal stem cells (MSC), and PPARγ was a key regulator in MSC osteogenic differentiation. However, whether bFGF and PPARγ could play synergetic roles within a 3D matrix to promote MSC osteogenic differentiation was unknown. In the study, bFGF and PPARγ targeting siRNAs were incorporated into collagen hydrogels for MSC cultivation. Their optimal concentrations in collagen hydrogels were determined. The capacity of bFGF/siRNA-carrying hydrogels in supporting osteogenic differentiation of MSCs was systematically evaluated with multimodality of methods, including flow cytometry, quantitative real-time PCR, Western Blotting, as well as ALP activity and calcium content determination. We demonstrated in 3D collagen hydrogel that both bFGF and siRNA molecules were efficiently retained, strengthening their effects on the incorporated MSCs. Osteogenic analysis demonstrated that the in-situ forming hydrogels carrying bFGF and siRNAs potently promoted osteogenic differentiation of incorporated MSCs, significantly superior to pure collagen and bFGF-carrying collagen. Thus, collagen hydrogels functionalized with bFGF and PPARγ targeting siRNAs may be promising in bone tissue engineering.

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Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner

Cited by 156 publications

References 22 publications

When nutrition interacts with osteoblast function: molecular mechanisms of polyphenols

When nutrition interacts with osteoblast function: molecular mechanisms of polyphenols

Icariin-Loaded TiO₂ Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

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Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner

Cited by 156 publications

References 22 publications

When nutrition interacts with osteoblast function: molecular mechanisms of polyphenols

When nutrition interacts with osteoblast function: molecular mechanisms of polyphenols

Icariin-Loaded TiO2 Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells

Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis

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Product

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Icariin-Loaded TiO₂ Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells