Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus‐Derived Mesenchymal Stem Cells

Chen, Sheng; Deng, Xueqing; Ma, Kaige; Zhao, Lei; Huang, Danfeng; Li, Zhiliang; Shao, Zengwu

doi:10.1155/2018/3459612

Cited by 16 publications

(15 citation statements)

References 50 publications

(56 reference statements)

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“…Because the direct treatment effects of TGF-b signaling have been introduced in detail above, the treatments of TGF-b combined with tissue engineering technology for IVD degeneration will be mainly discussed below. Mesenchymal stem cell (MSC)-based tissue engineering treatment has been widely contemplated for the repair of IVD degeneration and has shown promising perspectives 108,109 . Studies show that TGF-b plays a vital role in it 110,111 .…”

Section: The Treatment Prospect Of Tgf-b Signaling In Ivd Degenerationmentioning

confidence: 99%

TGF-β signaling in intervertebral disc health and disease

Chen

Liu

et al. 2019

Osteoarthritis and Cartilage

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Objective: This paper aims to provide a comprehensive review of the changing role of transforming growth factor-b (TGF-b) signaling in intervertebral disc (IVD) health and disease. Methods: A comprehensive literature search was performed using PubMed terms 'TGF-b' and 'IVD'. Results: TGF-b signaling is necessary for the development and growth of IVD, and can play a protective role in the restoration of IVD tissues by stimulating matrix synthesis, inhibiting matrix catabolism, inflammatory response and cell loss. However, excessive activation of TGF-b signaling is detrimental to the IVD, and inhibition of the aberrant TGF-b signaling can delay IVD degeneration. Conclusions: Activation of TGF-b signaling has a promising treatment prospect for IVD degeneration, while excessive activation of TGF-b signaling may contribute to the progression of IVD degeneration. Studies aimed at elucidating the changing role of TGF-b signaling in IVD at different pathophysiological stages and its specific molecular mechanisms are needed, and these studies will contribute to safe and effective TGF-b signaling-based treatments for IVD degeneration.

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Section: The Treatment Prospect Of Tgf-b Signaling In Ivd Degenerationmentioning

confidence: 99%

TGF-β signaling in intervertebral disc health and disease

Chen

Liu

et al. 2019

Osteoarthritis and Cartilage

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“…Many studies confirmed that MSCs derived from the NP, AF, and CEP have the ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages [35][36][37][38][39][40][41][42][43][44] . Liu et al [49] compared the characteristics of three types of MSCs derived from human IVD including NP-MSCs, AF-MSCs, and CEP-MSCs and found that they showed similar multilineage differentiation capacities, whereas CEP-MSCs showed the best migration and invasion potency.…”

Section: Multi-differentiationmentioning

confidence: 99%

“…The most often used dimethyl sulfoxide is regarded as the standard cryoprotectant which may cause cytotoxicity to MSCs [69,70] . A recent study by Chen et al [44] showed that the addition of Icariin known as antioxidant to the conventional freezing medium could improve the viability and function of cryopreserved human NP-MSCs, which may be a new method of preserving stem/progenitor cells in the IVD for ERS (Table 2).…”

Section: Refmentioning

confidence: 99%

Insights of stem cell-based endogenous repair of intervertebral disc degeneration

Yang

et al. 2020

WJSC

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Low back pain has become more prevalent in recent years, causing enormous economic burden for society and government. Common therapies used in clinics including conservative treatment and surgery can only relieve pain. Subsequent cell-based treatment such as mesenchymal stem cell transplantation poses problems such as short duration of therapeutic effect and tumorigenesis. Recently, the discovery and identification of stem cell niche and stem/progenitor cells in intervertebral disc bring increased attention to endogenous repair strategy. Therefore, we review the studies involving endogenous repair strategy and present the characteristics and current status of this treatment. Meanwhile, we also discuss the strategy and perspective of endogenous repair strategy in future.

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“…We and other researchers from different research groups have isolated, cultured, and identified endogenous IVD MSCs from nucleus pulposus (NP) [8], annulus fibrosus (AF) [9], cartilage endplate (CEP) [10], and stem cell niche areas in IVD [11]. These endogenous MSCs can serve as the seed cells for endogenous repair of IVD degeneration.…”

Section: Introductionmentioning

confidence: 99%

Heme Oxygenase-1-Mediated Autophagy Protects against Oxidative Damage in Rat Nucleus Pulposus-Derived Mesenchymal Stem Cells

Chen

Liu

Zhao

et al. 2020

Oxidative Medicine and Cellular Longevity

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Although endogenous nucleus pulposus-derived mesenchymal stem cell- (NPMSC-) based regenerative medicine has provided promising repair strategy for intervertebral disc (IVD) degeneration, the hostile microenvironments in IVD, including oxidative stress, can negatively affect the survival and function of the NPMSCs and severely hinder the endogenous repair process. Therefore, it is of great importance to reveal the mechanisms of the endogenous repair failure caused by the adverse microenvironments in IVD. The aim of this study was to investigate the effect of oxidative stress on the rat NPMSCs and its underlying mechanism. Our results demonstrated that oxidative stress inhibited cell viability, induced apoptosis, and increased the production of reactive oxygen species (ROS) in NPMSCs. In addition, the results showed that the expression level of heme oxygenase-1 (HO-1) increased at an early stage but decreased at a late stage when NPMSCs were exposed to oxidative stress, and the oxidative damages of NPMSCs could be partially reversed by promoting the expression of HO-1. Further mechanistic analysis indicated that the protective effect of HO-1 against oxidative damage in NPMSCs was mediated by the activation of autophagy. Taken together, our study revealed that oxidative stress could inhibit cell viability, induce apoptosis, and increase ROS production in NPMSCs, and HO-1-mediated autophagy might act as a protective response to the oxidative damage. These findings might enhance our understanding on the mechanism of the endogenous repair failure during IVD degeneration and provide novel research direction for the endogenous repair of IVD degeneration.

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Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus‐Derived Mesenchymal Stem Cells

Cited by 16 publications

References 50 publications

TGF-β signaling in intervertebral disc health and disease

TGF-β signaling in intervertebral disc health and disease

Insights of stem cell-based endogenous repair of intervertebral disc degeneration

Heme Oxygenase-1-Mediated Autophagy Protects against Oxidative Damage in Rat Nucleus Pulposus-Derived Mesenchymal Stem Cells

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