Icariin conjugated hyaluronic acid/collagen hydrogel for osteochondral interface restoration

Yang, Jun; Liu, Yanbo; He, Lihua; Wang, Qiguang; Wang, Lan; Yuan, Tun; Xiao, Yumei; Fan, Yujiang; Zhang, Xingdong

doi:10.1016/j.actbio.2018.05.005

Cited by 80 publications

(53 citation statements)

References 47 publications

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“…However, a slight inhibition of the proliferation of chondrocytes was observed at 100 µg/mL. Still, the experimental results at low concentration of ICA promoting cell proliferation and a certain degree of inhibition at high concentrations were consistent with the conclusions of Yang et al [33]. …”

Section: Resultssupporting

confidence: 90%

Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair

Kankala

Liu

et al. 2018

Materials

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In recent times, cartilage defects have been the most common athletic injuries, often leading to dreadful consequences such as osteoarthritis, pain, joint deformities, and other symptoms. It is also evident that damage to articular cartilage is often difficult to recover or self-heal because of poor vascular, nervous, and lymphatic supplies. Moreover, cartilage cells have poor regeneration ability and high maturity. Inspired by these facts and the rapid advances in the field of tissue engineering (TE), we fabricated highly porous three-dimensional (3D) scaffold architectures based on cell-responsive polymeric inks, i.e., sodium alginate and gelatin (SA-Gel, 1:3 ratio), by a novel 3D printing method. Moreover, the effect of various processing parameters was systematically investigated. The printed scaffolds of polymer composites gels with excellent transparency, moderate viscosity, and excellent fluid properties showed good surface morphology, better thermal stability and swelling effect, and unique interconnected porous architectures at the optimized operating parameters. In vitro cell proliferation experiments of these cytocompatible scaffolds showed the excellent adhesion rate and growth behavior of chondrocytes. In addition, the porous architectures facilitated the efficient distribution of cells with only a few remaining on the surface, which was confirmed by confocal laser scanning microscopic (CLSM) observations. Icariin (ICA) addition at a concentration of 10 μg/mL further significantly enhanced the proliferation of chondrocytes. We envision that these cell-responsive polymeric inks in the presence of growth regulators like ICA may have potential in engineering complex tissue constructs toward diverse applications in TE.

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Section: Resultssupporting

confidence: 90%

Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair

Kankala

Liu

et al. 2018

Materials

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“…1F). All these results suggested that both mechanical and oriented gradients could be regulated through tuning multiple factors simply, superior to gradient hydrogel systems reported previously (Wang et al, 2018b;Yang et al, 2018;Gao et al, 2019). The mechanical gradients of the hydrogels could be cover different tissues including skin, muscle, cartilage and bone, suggesting their promising applications across these tissues (Nonoyama et al, 2016;Oh et al, 2016;Lu et al, 2018;Yin et al, 2018).…”

Section: Research Articlementioning

confidence: 80%

“…To demonstrate the advantages of electric field-driven blocks in tissue engineering, the composite nanofiber hydrogels with suitable mechanical gradients were fabricated to facilitate the engineering of the osteochondral interface. Challenges remain to regenerate osteochondral interface due to the complex gradient niches that was difficult to be imitated for most hydrogel systems (Levingstone et al, Electric field-driven building blocks for introducing multiple gradients to hydrogels RESEARCH ARTICLE 2016; Liao et al, 2017;Di Donato et al, 2018;Yang et al, 2018). Cell fates could be determined by mechanical cues (Han et al, 2016;Wang et al, 2018a;Liu et al, 2019).…”

Section: Perpendicular Parallelmentioning

confidence: 99%

“…However, finer regulations of these gradients are still required to optimize the functional recovery of tissues. Although plenty of approaches have been developed to form biomaterial gradients (Wang et al, 2018a;Yang et al, 2018;Yin et al, 2018;Gao et al, 2019;Li et al, 2019), little studies could introduce the gradients of multiple cargos simultaneously due to the interference of different cargos in fabrication processes. A gap remains for the biomaterials with gradients and native microenvironments of tissues in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Electric field-driven building blocks for introducing multiple gradients to hydrogels

Ding

Lü

et al. 2020

Protein Cell

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Gradient biomaterials are considered as preferable matrices for tissue engineering due to better simulation of native tissues. The introduction of gradient cues usually needs special equipment and complex process but is only effective to limited biomaterials. Incorporation of multiple gradients in the hydrogels remains challenges. Here, betasheet rich silk nanofibers (BSNF) were used as building blocks to introduce multiple gradients into different hydrogel systems through the joint action of crosslinking and electric field. The blocks migrated to the anode along the electric field and gradually stagnated due to the solution-hydrogel transition of the systems, finally achieving gradient distribution of the blocks in the formed hydrogels. The gradient distribution of the blocks could be tuned easily through changing different factors such as solution viscosity, which resulted in highly tunable gradient of mechanical cues. The blocks were also aligned under the electric field, endowing orientation gradient simultaneously. Different cargos could be loaded on the blocks and form gradient cues through the same crosslinking-electric field strategy. The building blocks could be introduced to various hydrogels such as Gelatin and NIPAM, indicating the universality. Complex niches with multiple gradient cues could be achieved through the strategy. Silk-based hydrogels with suitable mechanical gradients were fabricated to control the osteogenesis and chondrogenesis. Chondrogenic-osteogenic gradient transition was obtained, which stimulated the ectopic osteochondral tissue regeneration in vivo. The versatility and highly controllability of the strategy as well as multifunction of the building blocks reveal the applicability in complex tissue engineering and various interfacial tissues.

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“…Jiang et al demonstrated superior chondrogenic differentiation of MSC embedded in Col compared to MSC only in a rat model [50]. On the other hand, HA has been combined with Col 1 or gelatin before with similar outcomes that chondrogenic differentiation is promoted in the composite compared to HA or Col only [51][52][53]. One limitation of the present technique is the shear-induce alignment, limiting the degrees of spatial freedom in fibril arrangement.…”

Section: Discussionmentioning

confidence: 91%

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

Schwab

Hélary

Richards

et al. 2020

Preprint

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Biofabrication is providing scientists and clinicians the ability to produce engineered tissues with desired shapes, chemical and biological gradients. Typical resolutions achieved with extrusion-based bioprinting are at the macroscopic level. However, for capturing the fibrillar nature of the extracellular matrix (ECM), it is necessary to arrange ECM components at smaller scales, down to the sub-micron and the molecular level. In this study, we introduce a (bio)ink containing hyaluronan (HA) as tyramine derivative (THA) and collagen (Col). Similarly to other connective tissues, in this (bio)ink Col is present in fibrillar form and HA as viscoelastic space filler. THA was enzymatically crosslinked under mild conditions allowing simultaneous Col fibrillogenesis, thus achieving a homogeneous distribution of Col fibrils within the viscoelastic HA-based matrix. THA-Col composite displayed synergistic properties in terms of storage modulus and shear-thinning, translating into good printability. Shear-induced alignment of the Col fibrils along the printing direction was achieved and quantified via immunofluorescence and second harmonic generation. Cell-free and cell-laden constructs were printed and characterized, analyzing the influence of the controlled microscopic anisotropy on cell behavior and chondrogenic differentiation. THA-Col showed cell instructive properties modulating hMSC adhesion, morphology and sprouting from spheroids stimulated by the presence and the orientation of Col fibers. Actin filament staining showed that hMSCs embedded into aligned constructs displayed increased cytoskeleton alignment along the fibril direction. Based on gene expression of cartilage/bone markers and matrix production, hMSCs embedded into the bioink displayed chondrogenic differentiation comparable or superior to standard pellet culture by means of proteoglycan production (Safranin O staining and proteoglycan quantification) as well as increase in cartilage related genes. The possibility of printing matrix components with control over microscopic alignment brings biofabrication one step closer to capturing the complexity of native tissues.

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Icariin conjugated hyaluronic acid/collagen hydrogel for osteochondral interface restoration

Cited by 80 publications

References 47 publications

Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair

Effect of Icariin on Engineered 3D-Printed Porous Scaffolds for Cartilage Repair

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell morphology and alignment

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