Icariin Attenuates OGD/R‐Induced Autophagy via Bcl‐2‐Dependent Cross Talk between Apoptosis and Autophagy in PC12 Cells

Abstract

Icariin (ICA), an active component of Epimedium brevicornum Maxim, exerts a variety of neuroprotective effects such as antiapoptosis. However, the mechanisms underlying antiapoptosis of ICA in neurons exposed to oxygen-glucose deprivation and reperfusion (OGD/R) are unclear. The B-cell lymphoma-2 (Bcl-2) protein family plays an important role in the regulation of apoptosis and autophagy through Bcl-2-dependent cross talk. Bcl-2 suppresses apoptosis by binding to Bax and inhibits autophagy by binding to Beclin-1 which is an autophagy related protein. In the present study, MTT result showed that ICA increased cell viability significantly in OGD/R treated PC12 cells (P < 0.01). Results of western blotting analysis showed that ICA increased Bcl-2 expression significantly and decreased expressions of Bax, cleaved Caspase-3, Beclin-1, and LC3-II significantly in OGD/R treated PC12 cells (P < 0.01). These results suggest that ICA protects PC12 cells from OGD/R induced autophagy via Bcl-2-dependent cross talk between apoptosis and autophagy.

Icariside II, a PDE5 Inhibitor, Suppresses Oxygen-Glucose Deprivation/Reperfusion-Induced Primary Hippocampal Neuronal Death Through Activating the PKG/CREB/BDNF/TrkB Signaling Pathway

“…Primary hippocampal neurons were subjected to OGD 1.5 h/R 24 h to mimic cerebral I/R injury in vitro as describe in the previous studies (Mo et al, 2016;Feng et al, 2018). In brief, the neurobasal-A culture medium was removed and changed with EBSS (without glucose), subsequently, the cells were put into the hypoxia chamber (STEMCELL) with an atmosphere of 5% CO 2 , 95% N 2 , and 37°C for 1.5 h. After OGD, the medium was transformed into normal culture medium, or different concentrations of ICS II (12.5, 25, 50 mM) or SIL(20 mM) with 5% CO 2 at 37°C for another 24 h. The specific PKG inhibitor Rp-8-Br-cGMP (30 mM) or TrkB inhibitor ANA-12 (10 mM) was added into the corresponding cells 2 h prior to OGD, respectively.…”

Icariin Ameliorate Thiram-Induced Tibial Dyschondroplasia via Regulation of WNT4 and VEGF Expression in Broiler Chickens

“…Normal GP development entails cartilage vascularization and mineralization followed by osteogenesis. However, TD is characterized by tibial metaphyseal cartilage cell proliferation, non-vascular, non-mineralized, and white opaque mass in the GP ( Li et al, 2008 ; Shahzad et al, 2014 ; Nabi et al, 2016a ). TD affected chickens show depression, reduction in physical activities, feeding and drinking, standing difficulty, gait inflexible, movement disorders, and wings support walking ( Jefferies et al, 2000 ; Shahzad et al, 2014 ).…”

Neuroprotective mechanism of low-dose sodium nitrite in oxygen-glucose deprivation model of cerebral ischemic stroke in PC12 cells

“…Studies have shown that, after OGD, the fall in mitochondrial pH provides appropriate conditions for the reduction of nitrite to NO (Butler and Ridd, 2004[ 11 ]). Nitrite-derived NO can prevent neuronal nuclear translocation of NF-κB, an apoptosis-related transcription factor, resulting in the down-regulation of NF-κB target genes that are generally involved in inflammatory response and apoptotic pathways (Miller and Megson, 2007[ 49 ]; Mo et al, 2016[ 50 ]).…”