“…Internalization of anti-CAM/NC occurs via a common pathway, CAM endocytosis, in a variety of CAM-positive cell types (22,36,37,40,41). Human umbilical vein EC (HUVEC) and an endothelial-like cell line, EAhy926 (13), were selected to study the uptake and trafficking of anti-ICAM/NC since they provided insights that have subsequently correlated with in vivo models for endothelial targeting of anti-ICAM/NC (37). HUVEC (Clonetics, San Diego, CA) and EAhy926 were cultured at 37°C, 5% CO 2, and 95% relative humidity in supplemented medium 199 or DMEM (GIBCO-BRL, Grand Island, NY), respectively (36).…”