ICAM-1 and iNOS Expression Increased in the Skin of Mice after Vaccination with γ-Irradiated Cercariae ofSchistosoma mansoni

Kalyanasundaram, Ramaswamy; He, Yi; Salafsky, B.

doi:10.1006/expr.1997.4178

Cited by 31 publications

(28 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Abundant evidences indicate that NO contributes to the host defense functions of mononuclear phagocytes (Nathan & Hibbs 1991, Sternberg & McGuigan 1992, Evans et al 1993). Referring to murine models of schistosomiasis, recently there has been a report of the increased iNOS expression in skin of mice vaccinated with cercariae of S. mansoni (Ramaswamy et al 1997). However, there have been no reports of an actual nitrite in vitro production by cells that were obtained from schistosome infected mice.…”

Section: Discussionmentioning

confidence: 99%

Immunization with PIII, a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, affects nitric oxide production by murine spleen cells

Oliveira

Carmo

Silva-Teixeira

et al. 1998

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Immunization with PIII, a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, affects nitric oxide production by murine spleen cells

Oliveira

Carmo

Silva-Teixeira

et al. 1998

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…Larval stages of the parasite (schistosomula) after gaining entry into the body remain in the skin for about 48 -72 h before migrating to the lungs (1)(2)(3). This stay in the skin potentially provides ample opportunity for the host immune system to mount an effective immune response against the migrating parasite.…”

mentioning

confidence: 99%

“…This stay in the skin potentially provides ample opportunity for the host immune system to mount an effective immune response against the migrating parasite. Yet the host fails to elicit any tissue response against the skin-residing schistosomula (3). Conversely, infection with ␥-irradiation-attenuated parasites, which are known to confer significant immunity against this infection, stimulated a marked inflammatory response in the skin, resulting in delayed migration of the parasite through the skin (2)(3)(4).…”

mentioning

confidence: 99%

“…Yet the host fails to elicit any tissue response against the skin-residing schistosomula (3). Conversely, infection with ␥-irradiation-attenuated parasites, which are known to confer significant immunity against this infection, stimulated a marked inflammatory response in the skin, resulting in delayed migration of the parasite through the skin (2)(3)(4). This delayed migration correlated with the development of IFN-␥-producing putative effector cells in the skin and draining lymph nodes (5,6).…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Role for Parasite-Induced PGE2 in IL-10-Mediated Host Immunoregulation by Skin Stage Schistosomula ofSchistosoma mansoni

Kalyanasundaram

Kumar

2000

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Significant quantities of PGE2 were produced by cercariae of Schistosoma mansoni following incubation with linoleic acid, a free fatty acid found on the surface of the skin. Cyclooxygenase (COX) 2 inhibitors failed to block this PGE2 production, suggesting that a different biochemical pathway may be involved in the production of PGE2 by the parasite. In addition, the parasites were also able to induce PGE2 and IL-10 from human and mouse keratinocytes. Analysis of mouse skin during skin migratory phases of infection confirmed these in vitro observations. COX2 inhibitors blocked the parasite-induced PGE2 and IL-10 from keratinocytes. Further analysis of the parasite secretions showed that the PGE2/IL-10-inducing effect was associated with a fraction <30 kDa molecular size. Addition of this fraction or parasite-stimulated keratinocyte culture supernatant to Con A-stimulated spleen cells resulted in the suppression of cell proliferation. This effect could be blocked by anti-IL-10 treatment. In sharp contrast, attenuation of the parasites with γ-irradiation significantly abrogated their ability to induce PGE2 or IL-10 from skin cells. Significance of IL-10 in host immunoregulation by skin stage schistosomula of S. mansoni was further confirmed by using IL-10-deficient mice. In these mice the normal subdued cutaneous reaction to the parasite was absent. Instead, a prominent cellular reaction occurred around the parasite, and there was considerable delay in parasitic migration through the skin. Thus these results suggest a key role for parasite-induced PGE2 in IL-10-dependent down-regulation of host immune responses in the skin.

show abstract

“…Despite tissue damage due to migrating parasites, very little inflammatory responses were seen in the skin. [2][3][4] Previous studies from our laboratory showed that this subdued inflammatory response was due to a 16-kDa protein (designated Sm16) present abundantly in the secretions of schistosomula. 5 In vitro studies demonstrated that Sm16 stimulates IL-1ra production in human keratinocytes and suppresses transcription of the proinflammatory cytokines IL-1␣ and IL-1␤.…”

Section: Introductionmentioning

confidence: 99%

Suppression of cutaneous inflammation by intradermal gene delivery

Rao

Kalyanasundaram

2002

Gene Ther

View full text Add to dashboard Cite

ICAM-1 and iNOS Expression Increased in the Skin of Mice after Vaccination with γ-Irradiated Cercariae ofSchistosoma mansoni

Cited by 31 publications

References 38 publications

Immunization with PIII, a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, affects nitric oxide production by murine spleen cells

Immunization with PIII, a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, affects nitric oxide production by murine spleen cells

A Role for Parasite-Induced PGE2 in IL-10-Mediated Host Immunoregulation by Skin Stage Schistosomula ofSchistosoma mansoni

Suppression of cutaneous inflammation by intradermal gene delivery

Contact Info

Product

Resources

About