Ibuprofen inhibits adhesiveness of monocytes to endothelium and reduces cellular oxidative stress in smokers and non‐smokers

Zapolska-Downar, Danuta; Naruszewicz, Marek; Zapolski-Downar, A.; Markiewski, Maciej M.; Bukowska, H.; Millo, Barbara

doi:10.1046/j.1365-2362.2000.00742.x

Cited by 28 publications

(15 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We have also studied the intensity of ROS production by peripheral blood monocytes as a marker of leukocyte activation [9]. ROS were produced in greater amounts both by resting and PMA-stimulated monocytes from patients with hypertension, in line with previous reports [6,8].…”

Section: Discussionsupporting

confidence: 83%

“…Contrary to this, smoking by hypertensive patients enhanced the expression of CD11a on monocytes. This finding appears logical because, as mentioned earlier, activation of monocytes is modulated by oxidative stress [9]. Cigarette smoke contains large amounts of free radicals that participate in pro-oxidant stress [43].…”

Section: Discussionmentioning

confidence: 79%

“…It is generally known that oxidative stress results from a disequilibrium between the production of free radicals, availability of antioxidants and activity of antioxidant enzymes. The possibility of a disequilibrium is supported by our previous study in healthy individuals showing that cigarette smoking is associated with increased production of superoxide anion but not of hydrogen peroxide [9], which appears in the final stage of the cascade of reactions that neutralize free radicals generated in the cell.…”

Section: Discussionmentioning

confidence: 84%

“…Dorffel et al [8] have found that monocytes isolated from hypertensive patients and subjected to the action of angiotensin II generate significantly more tumour necrosis factor-a and interleukin-1 than the same cells from healthy participants. Generation of reactive oxygen species (ROS) by monocytes is nowadays a recognized marker of activation of these cells [9]. Studies in hypertensive patients have demonstrated that monocytes freshly isolated from peripheral blood generate more ROS upon stimulation with phorbol-12-mistrate-13-acetate (PMA) or angiotensin II than control cells [6].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Is there an association between angiotensin-converting enzyme gene polymorphism and functional activation of monocytes and macrophage in young patients with essential hypertension?

Zapolska-Downar

Siennicka²,

Chełstowski³

et al. 2006

Journal of Hypertension

View full text Add to dashboard Cite

Background This study was undertaken to determine whether the phenotype of monocytes and monocytederived macrophages is more proatherogenic in young persons with arterial hypertension and whether this phenotype is affected by smoking or polymorphism of the angiotensin-converting enzyme (ACE) gene. MethodsWe enrolled 40 young patients (24.1 W 4.7 years) with previously untreated arterial hypertension and 40 agematched healthy controls. There were 20 smokers and 20 non-smokers in each group. ResultsIn the hypertensive group, we found enhanced monocyte expression of CD11a (P < 0.001), reduced expression of CD49d (P < 0.001) and CD62L (P < 0.005), greater oxidative stress in resting and phorbol-12-mistrate-13-acetate-stimulated monocytes (P < 0.001), enhanced adhesion of monocytes to endothelial cells (P < 0.001), greater expression of CD36 on monocyte-derived macrophages (P < 0.001), and enhanced production of reactive oxygen species by resting and phorbol-12-mistrate-13-acetate-stimulated macrophages (P < 0.001). Cigarette smoking by hypertensive patients was associated with enhanced (P < 0.002) CD11a expression. There were no associations of ACE gene polymorphism with cellular expression or reactive oxygen species production studied among hypertensive patients. Only CD62L expression in DD homozygote participants was higher (P < 0.039) than in II homozygote participants.Conclusions It is concluded that arterial hypertension affects the function of monocytes/macrophages in young persons. Polymorphism of the ACE gene is without effect on the functional activation of monocytes and macrophages.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Is there an association between angiotensin-converting enzyme gene polymorphism and functional activation of monocytes and macrophage in young patients with essential hypertension?

Zapolska-Downar

Siennicka²,

Chełstowski³

et al. 2006

Journal of Hypertension

View full text Add to dashboard Cite

show abstract

“…However, these effects apparently did not translate into increased HDL generation, as similar plasma HDL-C levels were noted in a small prospective study in aspirinand placebo-treated groups (Eritsland et al 1989). In one study, the administration of ibuprofen was found to slightly elevate HDL-C levels both in smokers and nonsmokers, whereas in another study treatment with naproxen failed to show any effect (Zapolska-Downar et al 2000;Young et al 1995). The influence of selective cyclooxygenase-2 inhibitors such as celecoxib or rofecoxib on HDL-C plasma levels has not been reported to date.…”

mentioning

confidence: 99%

Impact of Systemic Inflammation and Autoimmune Diseases on apoA-I and HDL Plasma Levels and Functions

Montecucco

Favari

Norata

et al. 2014

Handbook of Experimental Pharmacology

View full text Add to dashboard Cite

The cholesterol of high-density lipoproteins (HDLs) and its major proteic component, apoA-I, have been widely investigated as potential predictors of acute cardiovascular (CV) events. In particular, HDL cholesterol levels were shown to be inversely and independently associated with the risk of acute CV diseases in different patient populations, including autoimmune and chronic inflammatory disorders. Some relevant and direct anti-inflammatory activities of HDL have been also recently identified targeting both immune and vascular cell subsets. These studies recently highlighted the improvement of HDL function (instead of circulating levels) as a promising treatment strategy to reduce inflammation and associated CV risk in several diseases, such as systemic lupus erythematosus and rheumatoid arthritis. In these diseases, anti-inflammatory treatments targeting HDL function might improve both disease activity and CV risk. In this narrative review, we will focus on the pathophysiological relevance of HDL and apoA-I levels/functions in different acute and chronic inflammatory pathophysiological conditions.

show abstract

Protein profile in neuroblastoma cells incubated with S‐ and R‐enantiomers of ibuprofen by iTRAQ‐coupled 2‐D LC‐MS/MS analysis: Possible action of induced proteins on Alzheimer's disease

et al. 2008

View full text Add to dashboard Cite

Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, including Alzheimer's disease (AD). While its prophylactic effect on AD has been suggested, a comprehensive understanding of its mechanism of action remains unclear. Using iTRAQ-coupled 2-D LC-MS/MS analysis, we report here the first study of protein profiles of neuroblastoma cells incubated separately with the two enantiomers of ibuprofen. Three types of cellular proteins, including metabolic enzymes, signaling molecules and cytoskeletal proteins, displayed changes. The changes in the level of a number of enzymes involved in fatty acid synthesis and antioxidant activity in cells incubated with the S-enantiomer were further supported by the real-time PCR analysis as well as the reduced level of reactive oxygen species in cells incubated with the S-enantiomer of ibuprofen. Our findings, therefore, provide the possible mechanism of ibuprofen-induced proteins on AD, and the beneficial effects of ibuprofen in reducing the development of AD.

show abstract

Ibuprofen inhibits adhesiveness of monocytes to endothelium and reduces cellular oxidative stress in smokers and non‐smokers

Abstract: Oral administration of ibuprofen reduced the adhesion of monocytes to HUVEC, suppressed oxidative stress and increased HDL cholesterol levels in smokers and non-smokers.

Cited by 28 publications

References 43 publications

Is there an association between angiotensin-converting enzyme gene polymorphism and functional activation of monocytes and macrophage in young patients with essential hypertension?

Is there an association between angiotensin-converting enzyme gene polymorphism and functional activation of monocytes and macrophage in young patients with essential hypertension?

Impact of Systemic Inflammation and Autoimmune Diseases on apoA-I and HDL Plasma Levels and Functions

Protein profile in neuroblastoma cells incubated with S‐ and R‐enantiomers of ibuprofen by iTRAQ‐coupled 2‐D LC‐MS/MS analysis: Possible action of induced proteins on Alzheimer's disease

Contact Info

Product

Resources

About