Iatrogenic lesions and artefacts in gynaecological pathology

Clarke, Blaise A.; McCluggage, W. Glenn

doi:10.1136/jcp.2008.061424

Cited by 15 publications

(5 citation statements)

References 45 publications

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“…Like all histological techniques, HREM is an ex vivo method, which visualizes materials extracted from their natural environment [ 13 , 25 , 26 , 27 ]. Harvesting and manipulating the specimens causes artefacts that are already known from traditional histology.…”

Section: Discussionmentioning

confidence: 99%

Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM)

et al. 2021

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High resolution episcopic microscopy (HREM) produces digital volume data by physically sectioning histologically processed specimens, while capturing images of the subsequently exposed block faces. Our study aims to systematically define the spectrum of typical artefacts inherent to HREM data and to research their effect on the interpretation of the phenotype of wildtype and mutant mouse embryos. A total of 607 (198 wildtypes, 409 mutants) HREM data sets of mouse embryos harvested at embryonic day (E) 14.5 were systematically and comprehensively examined. The specimens had been processed according to essentially identical protocols. Each data set comprised 2000 to 4000 single digital images. Voxel dimensions were 3 × 3 × 3 µm3. Using 3D volume models and virtual resections, we identified a number of characteristic artefacts and grouped them according to their most likely causality. Furthermore, we highlight those that affect the interpretation of embryo data and provide examples for artefacts mimicking tissue defects and structural pathologies. Our results aid in optimizing specimen preparation and data generation, are vital for the correct interpretation of HREM data and allow distinguishing tissue defects and pathologies from harmless artificial alterations. In particular, they enable correct diagnosis of pathologies in mouse embryos serving as models for deciphering the mechanisms of developmental disorders.

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Section: Discussionmentioning

confidence: 99%

Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM)

et al. 2021

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“…Displaced granulosa cells, whether within the fallopian tube, ovarian stroma, or vascular channels have been variably mistaken for tumors (2), including small cell carcinoma (3), granulosa cell tumors (1), or metastases (4). When associated with an ovarian teratoma, these cells may be misinterpreted as immature neural elements.…”

Section: To the Editormentioning

confidence: 99%

Displaced Granulosa Cells Within the Ovarian Stroma in a BRCA1 Mutation Carrier

Chui

Chapman

Clarke

2014

International Journal of Gynecological Pathology

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“…The incidence of IVI has been recently increasing, most likely attributable to the introduction of percutaneous transluminal angioplasty and cardiac catheterization, [1][2][3][4][5][6] as well as the increased performance of cancer radical operations. [7][8][9][10] Endovascular therapy is a minimally invasive technique that has been widely used to treat various vascular diseases, especially abdominal and thoracic aortic aneurysms. [11][12][13][14] Recently, several studies have reported the management of IVIs with endovascular techniques.…”

Section: Introductionmentioning

confidence: 99%

A retrospective study on endovascular management of iatrogenic vascular injuries

Xiong

Guo

et al. 2012

Vascular

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The purpose of this study was to evaluate the outcome of endovascular management of iatrogenic vascular injuries (IVIs). We retrospectively reviewed the clinical records of 19 consecutive patients who underwent endovascular therapy of IVIs. Endograft implantation was conducted in 13 patients, intravascular embolization using coil was performed in four patients, combined treatment involving endovascular balloon intervention and percutaneous thrombin injection was done in one patient and the remaining one patient received balloon compression for hemostasis. There were no deaths after the operation. In the patients with vessel rupture (n = 9) or perforation (n = 4), cessation of bleeding and restoration of blood flow were achieved following the endovascular treatments. Four cases of pseudoaneurysm and one case of arteriovenous fistula were successfully eliminated. At a median follow-up of 23.8 months (range 484), one patient suffered a mild cerebral infarction secondary to covered stent placement and one patient developed a partial renal infarction secondary to coil embolization. Additionally, stent collapse was found in one patient with stenosis of the iliofemoral vein, accounting for the failure to resolve the lower limb edema. In conclusion, endovascular therapy is an effective technique in the management of different types of IVIs, but is associated with minor ischemic complications.

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Iatrogenic lesions and artefacts in gynaecological pathology

Cited by 15 publications

References 45 publications

Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM)

Artefacts in Volume Data Generated with High Resolution Episcopic Microscopy (HREM)

Displaced Granulosa Cells Within the Ovarian Stroma in a BRCA1 Mutation Carrier

A retrospective study on endovascular management of iatrogenic vascular injuries

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