Ia antigen expression on human epidermal Langerhans cells

Rowden, Geoffrey; Lewis, Martin; Sullivan, Arthur K.

doi:10.1038/268247a0

Cited by 539 publications

(124 citation statements)

References 19 publications

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“…All of these populations express easily detectable MHC class II antigens (1,24,25) and all appear to be bone marrow-derived, as indicated by the expression of the T 2 0 0 antigen; none of these populations show reactivity when stained for nonspecific esterase. Some minor differences between the DC populations were noted.…”

Section: Discussionmentioning

confidence: 99%

Dendritic cells with antigen-presenting capability reside in airway epithelium, lung parenchyma, and visceral pleura.

Sertl¹,

Takemura²,

Tschachler³

et al. 1986

The Journal of Experimental Medicine

340

184

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In this study, we identified a population of dendritic cells (DC) that exists throughout human and mouse pulmonary tissues, including the trachea, bronchi, alveoli, and visceral pleura. In human tissue, these DC were shown to be positive for HLA-DR and T200 antigens. In the mouse, the DC expressed not only Ia and the T200 antigen, but also Fc-IgG and C3bi receptors. Unlike alveolar macrophages, the DC were negative for nonspecific esterase staining and shared ultrastructural similarities with the DC described by Steinman (1), and with Langerhans' cells, even though they did not contain Birbeck granules. We were able to demonstrate that mouse pulmonary DC function in antigen presentation, as observed with the other DC. Thus, the respiratory tract contains DC that are capable of functioning in antigen presentation and that may be important in pulmonary immune responses.

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Section: Discussionmentioning

confidence: 99%

Dendritic cells with antigen-presenting capability reside in airway epithelium, lung parenchyma, and visceral pleura.

Sertl¹,

Takemura²,

Tschachler³

et al. 1986

The Journal of Experimental Medicine

340

184

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“…So-called Langerhans cells (LCs) have been shown to be the only cells in the epidermis to express MHC class II molecules under normal conditions (1,2). Upon antigen capture, LCs migrate to regional lymph nodes and are highly efficient at presenting MHC class II-restricted peptides to T cells (3,4).…”

Section: Introductionmentioning

confidence: 99%

Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells

Hunger¹,

Sieling²,

Ochoa³

et al. 2004

J. Clin. Invest.

247

104

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Langerhans cells (LCs) constitute a subset of DCs that initiate immune responses in skin.Using leprosy as a model, we investigated whether expression of CD1a and langerin, an LC-specific C-type lectin, imparts a specific functional role to LCs. LC-like DCs and freshly isolated epidermal LCs presented nonpeptide antigens of Mycobacterium leprae to T cell clones derived from a leprosy patient in a CD1a-restricted and langerin-dependent manner. LC-like DCs were more efficient at CD1a-restricted antigen presentation than monocyte-derived DCs. LCs in leprosy lesions coexpress CD1a and langerin, placing LCs in position to efficiently present a subset of antigens to T cells as part of the host response to human infectious disease.

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“…During this migration, they undergo a maturation process that allows them to present antigens to naive T cells (Kripke et al, 1990;Moll et al, 1993). Notably, Langerhans cells are the only epidermal cells to constitutively express major histocompatibility complex class II molecules (Klareskog et al, 1977;Rowden et al, 1977), CD1a molecules (Fithian et al, 1981), and Langerin (Valladeau et al, 2000) at their cell surface. In addition, Langerhans cells differ ultrastructurally from other dendritic cells through the presence of Birbeck granules (BGs), distinctive rod-shaped structures of variable length with a central, periodically striated lamella (Birbeck et al, 1961).…”

mentioning

confidence: 99%

Birbeck Granules Are Subdomains of Endosomal Recycling Compartment in Human Epidermal Langerhans Cells, Which Form Where Langerin Accumulates

Dermott

Ziylan

Spehner

et al. 2002

MBoC

166

155

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Birbeck granules are unusual rod-shaped structures specific to epidermal Langerhans cells, whose origin and function remain undetermined. We investigated the intracellular location and fate of Langerin, a protein implicated in Birbeck granule biogenesis, in human epidermal Langerhans cells. In the steady state, Langerin is predominantly found in the endosomal recycling compartment and in Birbeck granules. Langerin internalizes by classical receptor-mediated endocytosis and the first Birbeck granules accessible to endocytosed Langerin are those connected to recycling endosomes in the pericentriolar area, where Langerin accumulates. Drug-induced inhibition of endocytosis results in the appearance of abundant open-ended Birbeck granule-like structures appended to the plasma membrane, whereas inhibition of recycling induces Birbeck granules to merge with a tubular endosomal network. In mature Langerhans cells, Langerin traffic is abolished and the loss of internal Langerin is associated with a concomitant depletion of Birbeck granules. Our results demonstrate an exchange of Langerin between early endosomal compartments and the plasma membrane, with dynamic retention in the endosomal recycling compartment. They show that Birbeck granules are not endocytotic structures, rather they are subdomains of the endosomal recycling compartment that form where Langerin accumulates. Finally, our results implicate ADP-ribosylation factor proteins in Langerin trafficking and the exchange between Birbeck granules and other endosomal membranes. INTRODUCTIONLangerhans cells (LCs), the representatives of the dendritic cell lineage in the epidermis and mucosal tissues, capture antigen in the skin before migrating to the T-cell-dependent areas of draining lymph nodes. During this migration, they undergo a maturation process that allows them to present antigens to naive T cells (Kripke et al., 1990;Moll et al., 1993). Notably, Langerhans cells are the only epidermal cells to constitutively express major histocompatibility complex class II molecules (Klareskog et al., 1977;Rowden et al., 1977), CD1a molecules (Fithian et al., 1981), and Langerin (Valladeau et al., 2000) at their cell surface. In addition, Langerhans cells differ ultrastructurally from other dendritic cells through the presence of Birbeck granules (BGs), distinctive rod-shaped structures of variable length with a central, periodically striated lamella (Birbeck et al., 1961).Despite the use of "dynamic" electron microscope studies (reviewed in Schuler et al., 1991), Birbeck granules remain enigmatic. Specifically, conflicting theories exist regarding Article published online ahead of print. Mol. Biol. Cell 10.1091/ mbc.01-06-0300. Article and publication date are at www.molbiolcell.org/cgi/doi/10.1091/mbc.01-06-0300.† These authors contributed equally to this work and are listed in alphabetical order. # Corresponding author. E-mail address: daniel.hanau@efs-alsace.fr. Abbreviations used: Au, gold-labeled; BG, Birbeck granule; ERC, endosomal recycling compartment; Fi, freshl...

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Ia antigen expression on human epidermal Langerhans cells

Cited by 539 publications

References 19 publications

Dendritic cells with antigen-presenting capability reside in airway epithelium, lung parenchyma, and visceral pleura.

Dendritic cells with antigen-presenting capability reside in airway epithelium, lung parenchyma, and visceral pleura.

Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells

Birbeck Granules Are Subdomains of Endosomal Recycling Compartment in Human Epidermal Langerhans Cells, Which Form Where Langerin Accumulates

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