<i>β</i>‐Mannanase production by <scp><i>Aspergillus niger</i> BCC4525</scp> and its efficacy on broiler performance

Sornlake, Warasirin; Matetaviparee, Panida; Rattanaphan, Nakul; Tanapongpipat, Sutipa; Eurwilaichitr, Lily

doi:10.1002/jsfa.6183

Cited by 10 publications

(10 citation statements)

References 32 publications

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“…In accordance with the work of Sornlake et al . (2013), mannanase produced by Aspergillus niger BCC4525 also had a wide optimal pH range from 2 to 6. In Fig.…”

Section: Resultsmentioning

confidence: 99%

The activity of β‐mannanase in the commercial enzyme Pectinex Ultra SP‐L and its use for the production of mannooligosaccharides from copra meal

Chimpanao

Pruksasri

2022

Int J of Food Sci Tech

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The β-mannanase present in the commercial enzyme Pectinex Ultra SP-L was characterised and tested for its ability to hydrolyse copra meal for the production of mannooligosaccharides (MOS). The optimum pH and temperature for the mannanase activity were 4.0 and 65 °C, respectively. The relative activity of the β-mannanase of the commercial preparation Pectinex Ultra SP-L on copra meal was 49% when compared to the complete hydrolysis of locust bean gum, indicating its potential to hydrolyse the agricultural waste. The maximum MOS concentration of 25 g L −1 was obtained from the hydrolysis of 300 g L −1 copra meal with Pectinex Ultra SP-L at 55 °C for 2 h. The hydrolysate consisted of ~45% oligosaccharides and the rest were monosaccharides. Mannobiose and mannotriose were the only oligosaccharides present in the mixture, with mannobiose accounting for 90%. A simple and efficient method to obtain oligosaccharides from copra meal using a commercial pectinolytic enzyme is presented.

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“…In accordance with the work of Sornlake et al . (2013), mannanase produced by Aspergillus niger BCC4525 also had a wide optimal pH range from 2 to 6. In Fig.…”

Section: Resultsmentioning

confidence: 99%

The activity of β‐mannanase in the commercial enzyme Pectinex Ultra SP‐L and its use for the production of mannooligosaccharides from copra meal

Chimpanao

Pruksasri

2022

Int J of Food Sci Tech

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“…The in vitro digestion test was performed as described by Sornlake et al, 2014. 9) The in vitro digestion model simulated the three sections of the digestive tract (crop, proventriculus/gizzard, and duodenum, respectively) of chickens fed acornbased diet. One gram of the corn-based diet was added with 20% non-pretreated PKM in sodium acetate buffer pH 5.7 in triplicate.…”

Section: Biomass Hydrolysis and Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

“…Recently, an isolate of Aspergillus niger from BIO-TEC Culture Collection (BCC) was reported to produce high levels of β-mannanase. 9) The crude β-mannanase preparation from this strain had potential value as a feed supplement in an animal field trial. In this study, cDNA encoding β-mannanase was isolated from the fungal strain and cloned into KM71 Pichia pastoris.…”

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confidence: 98%

“…8) For example, β-mannanase is used to hydrolyze mannan-based polysaccharides into shorter oligosaccharides in animal feed, and this enzyme could be beneficial in some circumstances for broiler gut health. 9) Moreover, agricultural wastes are increasingly used as renewable biomass sources for production of valueadded feedstocks, chemicals, or biofuels. In various countries like Asia and Africa, agricultural wastes such as palm kernel meal (PKM) and copra meal (CM) contain relatively high levels of mannans.…”

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confidence: 99%

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Production of high activity Aspergillus niger BCC4525 β-mannanase in Pichia pastoris and its application for mannooligosaccharides production from biomass hydrolysis

Harnpicharnchai

Pinngoen

Teanngam

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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A cDNA encoding β-mannanase was cloned from Aspergillus niger BCC4525 and expressed in Pichia pastoris KM71. The secreted enzyme hydrolyzed locust bean gum substrate with very high activity (1625 U/mL) and a relatively high k/K (461 mg s mL). The enzyme is thermophilic and thermostable with an optimal temperature of 70 °C and 40% retention of endo-β-1,4-mannanase activity after preincubation at 70 °C. In addition, the enzyme exhibited broad pH stability with an optimal pH of 5.5. The recombinant enzyme hydrolyzes low-cost biomass, including palm kernel meal (PKM) and copra meal, to produce mannooligosaccharides, which is used as prebiotics to promote the growth of beneficial microflora in animals. An in vitro digestibility test simulating the gastrointestinal tract system of broilers suggested that the recombinant β-mannanase could effectively liberate reducing sugars from PKM-containing diet. These characteristics render this enzyme suitable for utilization as a feed additive to improve animal performance.

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“…Thus, many feed additives have emerged in poultry nutrition such as probiotics [17], prebiotics [18] [19], microelements [20] [21], digestive enzymes [22] [22] plants extracts or essential oils [24] [25] [26] and clays [27] [28] [29]. Particularly, among clays, action of ion-exchanged clays on microbiota is well described.…”

Section: Introductionmentioning

confidence: 99%

Effect of Zeolite on Small Intestine Microbiota of Broiler Chickens: A Case Study

Gall‐David¹,

Meuric²,

Benzoni³

et al. 2017

FNS

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After ban of antibiotics growth promoters (AGP) in Europe in 2006, use of non-medicated gut microbiota regulators as feed additives has dramatically increased. This study aimed at describing the effects of a copper-exchanged zeolite on broiler growth performance, small intestine morphology and microbiota composition. Illumina Sequencing of the V3-V4 region of the 16S rRNA gene was employed to study the small intestine microbiota. This microbiota with copper-exchanged zeolite treated-chickens was significantly less diverse with an almost exclusive presence of Lactobacillus johnsonii and Lactobacillus reuteri. These Lactobacilli are correlated with increased diameter, length and weight of the three segments of the small intestine and decreased viscosity of the intestinal content, suggesting probiotic action. The tested copper-exchanged zeolite would act as a prebiotic, selecting a "favorable" flora for the healthy broilers development.

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β‐Mannanase production by Aspergillus niger BCC4525 and its efficacy on broiler performance

Abstract: A high level of β-mannanase was produced by A. niger BCC4525 under solid state condition using copra meal as carbon source. Although the enzyme has the desired properties of an enzyme additive for improving broiler performance, it does not appear to be beneficial.

Cited by 10 publications

References 32 publications

The activity of β‐mannanase in the commercial enzyme Pectinex Ultra SP‐L and its use for the production of mannooligosaccharides from copra meal

The activity of β‐mannanase in the commercial enzyme Pectinex Ultra SP‐L and its use for the production of mannooligosaccharides from copra meal

Production of high activity Aspergillus niger BCC4525 β-mannanase in Pichia pastoris and its application for mannooligosaccharides production from biomass hydrolysis

Effect of Zeolite on Small Intestine Microbiota of Broiler Chickens: A Case Study

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