<i>β</i>-Glucosidase immobilisation on synthetic superparamagnetic magnetite nanoparticles and their application in saccharification of wheat straw and<i>Eucalyptus globulus</i>pulps

Valenzuela, Roberto; Castro, Jean F.; Parra, Carolina; Baeza, Jaime; Durán, Nélson; Freer, Juanita

doi:10.1080/17458080.2011.651167

Cited by 19 publications

(8 citation statements)

References 21 publications

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“…Latex-immobilized BGL hydrolyzed the cellulosic substrates at higher rates than did silicone-immobilized BGL. Similar effects of the increased hydrolysis rate of a number of cellulosic substrates by the addition of immobilized BGL on various supports have been reported earlier Karagulyan et al, 2008;Tu et al, 2006;Valenzuela et al, 2014;Zheng et al, 2013).…”

Section: Discussionsupporting

confidence: 74%

“…To date, the immobilization of BGL had been carried out in/on various carriers, including kaolin (Karagulyan et al, 2008), silica gel (Jung et al, 2012), Eupergit C (Khan, Lindahl, Turner, & Karlsson, 2012), iron oxide (Valenzuela et al, 2014), chitosan (Chang et al, 2013), nanoparticles (Cipolatti et al, 2014;Verma et al, 2013), streptavidin-coated magnetic particles (Alftrén, Ottow, & Hobley, 2013), mesoporous titanium oxide (Wei et al, 2013), sol-gel (Figueira et al, 2011(Figueira et al, , 2013, glass microspheres and nylon (Chen et al, 2012). However, for cost effective immobilization of enzymes, much cheaper matrices and a simple but effective stabilization approach should be utilized.…”

Section: Introductionmentioning

confidence: 98%

“…Enzymatic degradation of biomass involves the synergistic action of three main categories of cellulases, which include endo-1,4-b-D-glucanase (EC 3.2.1.4), exo-1,4-b-D-glucanase (EC 3.2.1.91) and b-glucosidase (EC 3.2.1.21). Several bacteria and fungi, including aerobes or anaerobes, mesophiles and thermophiles, are known for the production of these cellulolytic enzymes (Figueira, Sato, & Fernandes, 2013;Valenzuela et al, 2014;Zheng, Wang, Lu, Xu, & Sun, 2013). However, the level of extracellular cellulases from bacteria and fungi, which are capable of solubilizing crystalline cellulose extensively, is generally low.…”

Section: Introductionmentioning

confidence: 99%

“…Bioconversion of lignocellulosic biomass to biofuels, through enzymatic hydrolysis, has become a critical strategy to reduce our dependence on fossil fuels (Valenzuela et al, 2014;Verma, Chaudhary, Tsuzuki, Barrow, & Puri, 2013). Enzymatic degradation of biomass involves the synergistic action of three main categories of cellulases, which include endo-1,4-b-D-glucanase (EC 3.2.1.4), exo-1,4-b-D-glucanase (EC 3.2.1.91) and b-glucosidase (EC 3.2.1.21).…”

Section: Introductionmentioning

confidence: 99%

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Cost-efficient entrapment of β-glucosidase in nanoscale latex and silicone polymeric thin films for use as stable biocatalysts

et al. 2016

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cost-efficient entrapment of β-glucosidase in nanoscale latex and silicone polymeric thin films for use as stable biocatalysts

et al. 2016

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“…Accessory enzymes, including hemicellulose debranching enzymes such as arabinofuranosidases, glucuronidases, galactosidases, and feruloyl esterases can be as well added to assist in saccharification. Nevertheless, even when a high number of hydrolases of different origin are synergistically combined, complete saccharification is not achieved, which points out the complexity of enzyme synergism and biomass recalcitrance. On the other hand, many commercial grade enzymes have been observed to exert side activities .…”

Section: Enzymatic‐assisted Extraction Of Plant Polysaccharides In a mentioning

confidence: 99%

Enzymatic‐assisted extraction and modification of lignocellulosic plant polysaccharides for packaging applications

Martínez‐Abad

Ruthes

Vilaplana

2015

J of Applied Polymer Sci

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On the use of tris(nonylphenyl) phosphite as a chain extender in melt-blended poly(hydroxybutyrate-co-hydroxyvalerate)/ clay nanocomposites: Morphology, thermal stability, and mechanical properties J. González-Ausejo, E. Sánchez-Safont, J. Gámez-Pérez and L. Cabedo, J. Appl. Polym. Sci. 2015, DOI: 10.1002 Characterization of polyhydroxyalkanoate blends incorporating unpurified biosustainably produced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) A. Martínez-Abad, L. Cabedo, C. S. S. Oliveira, L. Hilliou, M. Reis and J. M. Lagarón, J. Appl. Polym. Sci. 2015, DOI: 10.1002 Modification of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) properties by reactive blending with a monoterpene derivative L. Pilon and C. Kelly, J. Appl. Polym. Sci. 2015, DOI: 10.1002 Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) films for food packaging: Physical-chemical ABSTRACT: Plant polysaccharides comprise the main renewable resource available in the biosphere for biomaterial production. However, the recalcitrant and heterogeneous structure of lignocellulosic biomass hinders the effective fractionation and exploitation of the polysaccharide components for the design of carbohydrate-based materials. Carbohydrate-active enzymes constitute a selective and versatile biotechnological tool that can assist during the biomass pretreatment steps to extract and fractionate the polysaccharide macromolecular components. Moreover, this enzymatic toolbox can be as well exploited for the tailored modification of the molecular structure of relatively pure polysaccharide components to achieve customized macroscopic properties. This review critically discusses the potential and challenges of the use of plant lignocellulosic polysaccharides and enzymatic modifications to design and prepare suitable materials for packaging applications in terms of their structure-property relations. Structural factors such as the molar mass and crystallinity of the polysaccharide fractions and functional factors such as water sensitivity and processability of the derived films are critical for the material performance. The global net primary production of carbon in the biosphere is estimated around 10 11 tonnes per year, 1 from which polysaccharides account for approximately 75% of all biomass. In this context, plant biomass represents the main renewable resource for biofuel and materials production, as a future replacement of fossil-based energy and goods. New schemes for the sustainable exploitation of biomass have emerged in the last decades (the "biorefinery" concept), which integrate chemical, mechanical, thermal, and biotechnological conversion processes for the generation of energy, platform chemicals, and bio-based polymeric materials. Different generations of biorefineries have been implemented, depending on the biomass source (e.g., agriculturederived crops and food waste, lignocellulosic biomass, marine feedstock, designer crops) and on the diversity of products that are converted (e.g., bioethanol after fermentation, syngas after thermochemical conversion, fin...

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A High‐Gradient Magnetic Separator for Highly Viscous Process Liquors in Industrial Biotechnology

et al. 2016

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Following the demand for highly efficient processes in biotechnology, an innovative and cost‐effective permanent magnet‐based high‐gradient magnetic separator with static magnetic filters (SMF) is presented. The laboratory‐scale prototype has a high particle capacity and is characterized by an outstanding separation performance. Low‐cost magnetic nanoparticles (MNPs, hydrodynamic diameter 100 – 200 nm) were separated from highly viscous sucrose solutions. Losses of MNPs with filtrate remained very low. The in situ implementation of ultrasound technology during MNP‐backwashing yielded excellent MNP recovery efficiencies. By applying a non‐stick coating onto the SMF surface, the MNP recovery efficiency could be enhanced significantly compared to conventional SMFs.

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β-Glucosidase immobilisation on synthetic superparamagnetic magnetite nanoparticles and their application in saccharification of wheat straw andEucalyptus globuluspulps

Cited by 19 publications

References 21 publications

Cost-efficient entrapment of β-glucosidase in nanoscale latex and silicone polymeric thin films for use as stable biocatalysts

Cost-efficient entrapment of β-glucosidase in nanoscale latex and silicone polymeric thin films for use as stable biocatalysts

Enzymatic‐assisted extraction and modification of lignocellulosic plant polysaccharides for packaging applications

A High‐Gradient Magnetic Separator for Highly Viscous Process Liquors in Industrial Biotechnology

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