<i>Zp4</i> is completely dispensable for fertility in female rats

Zeng, Ming‐Hua; Wang, Yan; Huang, Haowen; Quan, Ru-Ping; Yang, Junting; Guo, Dan; Sun, Ying; Lv, Chao; Li, Tianying; Wang, Le; Tan, Hang-Jin; Long, Panpan; Deng, Hong‐Wen; Xiao, Hong‐Mei

doi:10.1093/biolre/ioab047

Cited by 9 publications

(6 citation statements)

References 29 publications

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“…In addition, sperm binding is normal in ZP4-knock out rabbits [ 32 ]. ZP4 is also dispensable for rat fertilisation [ 33 ]. However, recent work has suggested that ZP4 functions diverge amongst vertebrates [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Sperm-Binding Sites in the N-Terminal Domain of Bovine Egg Coat Glycoprotein ZP4

Dilimulati

Orita

Yonahara

et al. 2022

IJMS

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The species-selective interaction between sperm and egg at the beginning of mammalian fertilisation is partly mediated by a transparent envelope called the zona pellucida (ZP). The ZP is composed of three or four glycoproteins (ZP1–ZP4). The functions of the three proteins present in mice (ZP1–ZP3) have been extensively studied. However, the biological role of ZP4, which was found in all other mammals studied so far, has remained largely unknown. Previously, by developing a solid support assay system, we showed that ZP4 exhibits sperm-binding activity in bovines and the N-terminal domain of bovine ZP4 (bZP4 ZP-N1 domain) is a sperm-binding region. Here, we show that bovine sperm bind to the bZP4 ZP-N1 domain in a species-selective manner and that N-glycosylation is not required for sperm-binding activity. Moreover, we identified three sites involved in sperm binding (site I: from Gln-41 to Pro-46, site II: from Leu-65 to Ser-68 and site III: from Thr-108 to Ile-123) in the bZP4 ZP-N1 domain using chimeric bovine/porcine and bovine/human ZP4 recombinant proteins. These results provide in vitro experimental evidence for the role of the bZP4 ZP-N1 domain in mediating sperm binding to the ZP.

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Section: Discussionmentioning

confidence: 99%

Identification of Sperm-Binding Sites in the N-Terminal Domain of Bovine Egg Coat Glycoprotein ZP4

Dilimulati

Orita

Yonahara

et al. 2022

IJMS

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“…However, in vivo development was severely impaired in embryos covered by a ZP4-devoid zone. In addition, two recent studies in rats showed that ZP4 is not responsible for gamete-specific interaction [69,70].…”

Section: Genome-wide Association Study (Gwas) and Functional Analysismentioning

confidence: 99%

Estimation of the Genetic Components of (Co)variance and Preliminary Genome-Wide Association Study for Reproductive Efficiency in Retinta Beef Cattle

Jiménez¹,

Morales

Menéndez-Buxadera

et al. 2023

Animals

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In this study, we analyzed the variation of reproductive efficiency, estimated as the deviation between the optimal and real parity number of females at each stage of the cow’s life, in 12,554 cows belonging to the Retinta Spanish cattle breed, using classical repeatability and random regression models. The results of the analyses using repeatability model and the random regression model suggest that reproductive efficiency is not homogeneous throughout the cow’s life. The h2 estimate for this model was 0.30, while for the random regression model it increased across the parities, from 0.24 at the first calving to 0.51 at calving number 9. Additionally, we performed a preliminary genome-wide association study for this trait in a population of 252 Retinta cows genotyped using the Axiom Bovine Genotyping v3 Array. The results showed 5 SNPs significantly associated with reproductive efficiency, located in two genomic regions (BTA4 and BTA28). The functional analysis revealed the presence of 5 candidate genes located within these regions, which were previously involved in different aspects related to fertility in cattle and mice models. This new information could give us a better understanding of the genetic architecture of reproductive traits in this species, as well as allow us to accurately select more fertile cows.

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“…Many researchers have not found a relationship between the pathogenic variants in ZP4 and infertility [ 51 , 53 ]. The ZP morphology in Zp4−/− rats was normal [ 80 ]. Thus, to date, there is not enough data on the direct role of the ZP4 gene in the etiology of disturbance in the formation of the ZP.…”

Section: Genes Involved In the Formation Of The Zona Pellucida And As...mentioning

confidence: 99%

Genetics of Oocyte Maturation Defects and Early Embryo Development Arrest

Соловова

Chernykh

2022

Genes

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Various pathogenic factors can lead to oogenesis failure and seriously affect both female reproductive health and fertility. Genetic factors play an important role in folliculogenesis and oocyte maturation but still need to be clarified. Oocyte maturation is a well-organized complex process, regulated by a large number of genes. Pathogenic variants in these genes as well as aneuploidy, defects in mitochondrial genome, and other genetic and epigenetic factors can result in unexplained infertility, early pregnancy loss, and recurrent failures of IVF/ICSI programs due to poor ovarian response to stimulation, oocyte maturation arrest, poor gamete quality, fertilization failure, or early embryonic developmental arrest. In this paper, we review the main genes, as well as provide a description of the defects in the mitochondrial genome, associated with female infertility.

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Zp4 is completely dispensable for fertility in female rats

Cited by 9 publications

References 29 publications

Identification of Sperm-Binding Sites in the N-Terminal Domain of Bovine Egg Coat Glycoprotein ZP4

Identification of Sperm-Binding Sites in the N-Terminal Domain of Bovine Egg Coat Glycoprotein ZP4

Estimation of the Genetic Components of (Co)variance and Preliminary Genome-Wide Association Study for Reproductive Efficiency in Retinta Beef Cattle

Genetics of Oocyte Maturation Defects and Early Embryo Development Arrest

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