Zinc Finger Protein5Is Required for the Control of Trichome Initiation by Acting Upstream ofZinc Finger Protein8in Arabidopsis      

Abstract

Arabidopsis (Arabidopsis thaliana) trichome development is a model system for studying cell development, cell differentiation, and the cell cycle. Our previous studies have shown that the GLABROUS INFLORESCENCE STEMS (GIS) family genes, GIS, GIS2, and ZINC FINGER PROTEIN8 (ZFP8), control shoot maturation and epidermal cell fate by integrating gibberellins (GAs) and cytokinin signaling in Arabidopsis. Here, we show that a new C2H2 zinc finger protein, ZFP5, plays an important role in controlling trichome cell development through GA signaling. Overexpression of ZFP5 results in the formation of ectopic trichomes on carpels and other inflorescence organs. zfp5 loss-of-function mutants exhibit a reduced number of trichomes on sepals, cauline leaves, paraclades, and main inflorescence stems in comparison with wild-type plants. More importantly, it is found that ZFP5 mediates the regulation of trichome initiation by GAs. These results are consistent with ZFP5 expression patterns and the regional influence of GA on trichome initiation. The molecular analyses suggest that ZFP5 functions upstream of GIS, GIS2, ZFP8, and the key trichome initiation regulators GLABROUS1 (GL1) and GL3. Using a steroid-inducible activation of ZFP5 and chromatin immunoprecipitation experiments, we further demonstrate that ZFP8 is the direct target of ZFP5 in controlling epidermal cell differentiation.Cell differentiation and morphogenesis at appropriate times and places are critical for the normal development of multicellular organisms (Ishida et al., 2008).

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

“…ZFP2 was shown to control floral abscission, as overexpressing plants enhanced the retention of floral organs (Cai and Lashbrook, 2008). ZFP5, ZFP6, and ZFP8 were shown to regulate the differentiation of trichomes through integrating GA and cytokinin signaling (Zhou et al, 2011(Zhou et al, , 2013. We did not observe abnormalities in the trichome morphology of ZFP3ox or zfp3 mutant seedlings (data not shown).…”

Zinc-Finger Transcription Factor ZAT6 Positively Regulates Cadmium Tolerance through the Glutathione-Dependent Pathway in Arabidopsis

“…C 2 H 2 zincfinger proteins have been shown to be required for key cellular processes including transcriptional regulation, development, pathogen defense, and stress responses (Laity et al, 2001;Englbrecht et al, 2004;Ciftci-Yilmaz and Mittler, 2008;Zhou et al, 2011). ZAT6 contains a typical C 2 H 2 domain and has been shown to be induced by cold and osmotic stress (Vogel et al, 2005).…”

NTL8 Regulates Trichome Formation in Arabidopsis by Directly Activating R3 MYB Genes TRY and TCL1

“…Data represent the mean 6 SD of 18 plants. Yu et al, 2010;Zhou et al, 2011Zhou et al, , 2013Sun et al, 2015). To examine if this is the case for NTL8, we tested, by using qRT-PCR, the expression of the MBW complex component genes TTG1, GL1, GL3, and EGL3, and their target genes GL2 and singlerepeat R3 MYB genes TRY, CPC, ETC1, ETC2, ETC3, TCL1, and TCL2 in the ntl8-1D mutant seedlings.…”

“…The C2H2 transcription factors GLABROUS INFLORES-CENCE STEMS (GIS), GIS2, and GIS3, the ZINC FINGER transcription factors ZINC FINGER PROTEIN5 (ZFP5), ZFP6, and ZFP8, and the plant-specific transcription factor SQUAMOSA PROMOTER BINDING PROTEIN LIKE9 (SPL9) have been identified as regulators of trichome formation (Gan et al, 2006(Gan et al, , 2007Yu et al, 2010;Zhou et al, 2011Zhou et al, , 2013Sun et al, 2015). Most of these transcription factors regulate trichome formation via regulating, directly or indirectly, the expression of the key transcription factor genes.…”

“…Most of these transcription factors regulate trichome formation via regulating, directly or indirectly, the expression of the key transcription factor genes. For example, GIS, GIS3, ZFP5, and ZFP8 can regulate the expression of the MBW complex component genes (Gan et al, 2006(Gan et al, , 2007Zhou et al, 2011Zhou et al, , 2013Sun et al, 2015), whereas SPL9 can directly regulate the expression of the R3 MYB transcription factor genes TRY and TCL1 (Yu et al, 2010).…”