Y75B8A.8 (HC8) protein of Haemonchus contortus: A functional inhibitor of host IL‐2

Wang, Wenjuan; Wang, Yujian; Tian, Xiaowei; Lu, Mingmin; Ehsan, Muhammad; Ren, Yan; Song, Xiaoling; Xu, Lixin; Li, Xiangrui

doi:10.1111/pim.12625

Cited by 11 publications

(2 citation statements)

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“…The protein bears features of intrinsically disordered proteins, including polyglutamine stretches in the N-terminal half ( https://wormbase.org/species/c_elegans/protein/CE34135 #065-−10). The homologous protein in the parasitic nematode Haemonchus contortus is found in excretory and secretory products and is able to bind the interleukin IL2 of its mammalian host ( Wang et al, 2019 ). In C. elegans , the 3’UTR of Y75B8A.8 regulates RNA editing of the ADSR-2 mRNA ( Wheeler et al, 2015 ; Washburn and Hundley, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

A broad mutational target explains a fast rate of phenotypic evolution

Besnard

Picao-Osorio

Dubois

et al. 2020

eLife

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The rapid evolution of a trait in a clade of organisms can be explained by the sustained action of natural selection or by a high mutational variance, that is the propensity to change under spontaneous mutation. The causes for a high mutational variance are still elusive. In some cases, fast evolution depends on the high mutation rate of one or few loci with short tandem repeats. Here, we report on the fastest evolving cell fate among vulva precursor cells in Caenorhabditis nematodes, that of P3.p. We identify and validate causal mutations underlying P3.p's high mutational variance. We find that these positions do not present any characteristics of a high mutation rate, are scattered across the genome and the corresponding genes belong to distinct biological pathways. Our data indicate that a broad mutational target size is the cause of the high mutational variance and of the corresponding fast phenotypic evolutionary rate.

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Section: Resultsmentioning

confidence: 99%

A broad mutational target explains a fast rate of phenotypic evolution

Besnard

Picao-Osorio

Dubois

et al. 2020

eLife

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“…The interaction of HcADRM1 with goat T cells was investigated as previously described [ 26 ]. In brief, freshly sorted T cells were cultured with or without 5 µg/ml rHcADRM1 proteins for 2 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Unveiling the immunomodulatory properties of Haemonchus contortus adhesion regulating molecule 1 interacting with goat T cells

Tian

Zhang

et al. 2020

Parasites Vectors

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Background Gastrointestinal nematodes could release excretory-secretory (ES) proteins into the host environment to ensure their survival. These ES proteins act as immunomodulators to suppress or subvert the host immune response via the impairment of immune cell functions, especially in chronic infections. In our preliminary study, Haemonchus contortus adhesion-regulating molecule 1 (HcADRM1) was identified from H. contortus ES proteins (HcESPs) that interacted with host T cells via liquid chromatography-tandem mass spectrometry analysis. However, little is known about HcADRM1 as an ES protein which may play a pivotal role at the parasite-host interface. Methods Based on bioinformatics approaches, multiple amino acid sequence alignment was conducted and the evolutionary relationship of HcADRM1 with ADRM1 orthologues was extrapolated. Employing RT-qPCR and immunohistochemistry assays, temporal transcriptional and spatial expression profiles of HcADRM1 were investigated. Using immunostaining approaches integrated with immunological bioassays, the immunomodulatory potentials of HcADRM1 on goat T cells were assessed. Results We hereby demonstrated that HcADRM1 with immunodiagnostic utility was a mammalian ADRM1 orthologue abundantly expressed at all developmental stages of H. contortus . Given the implications of ADRM1 proteins in cell growth, survival and development, we further investigated the immunomodulatory property of HcADRM1 as an individual ES protein acting at the parasite-host interface. The rHcADRM1 stimuli notably suppressed T cell viability, promoted intrinsic and extrinsic T cell apoptosis, inhibited T cell proliferation and induced cell cycle arrest at G1 phase. Simultaneously, rHcADRM1 stimuli exerted critical controls on T cell cytokine secretion profiles, predominantly by restraining the secretions of interleukin (IL)-4, IL-10 and interferon-gamma. Conclusions Importantly, HcADRM1 protein may have prophylactic potential for anti- H. contortus vaccine development. Together, these findings may contribute to the clarification of molecular and immunomodulatory traits of ES proteins, as well as improvement of our understanding of parasite immune evasion mechanism in H. contortus -host biology.

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HcFAR, a functional inhibitor of goat TGF-β1 identified from excretory and secretory products of Haemonchus contortus

Cao

Tian

et al. 2020

Veterinary Parasitology

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Y75B8A.8 (HC8) protein of Haemonchus contortus: A functional inhibitor of host IL‐2

Cited by 11 publications

References 50 publications

A broad mutational target explains a fast rate of phenotypic evolution

A broad mutational target explains a fast rate of phenotypic evolution

Unveiling the immunomodulatory properties of Haemonchus contortus adhesion regulating molecule 1 interacting with goat T cells

HcFAR, a functional inhibitor of goat TGF-β1 identified from excretory and secretory products of Haemonchus contortus

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