WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens

Sakakibara, Keiko; Reisewitz, Pascal; Aoyama, Tsuyoshi; Friedrich, Thomas; Ando, Sayuri; Sato, Yoshikatsu; Tamada, Yosuke; Nishiyama, Tomoaki; Hiwatashi, Yuji; Kurata, Takeshi; Ishikawa, Masaki; Deguchi, Hiroyuki; Rensing, Stefan A.; Werr, Wolfgang; Murata, Takashi; Hasebe, Mitsuyasu; Laux, Thomas

doi:10.1242/dev.097444

Cited by 125 publications

(122 citation statements)

References 65 publications

(110 reference statements)

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“…P. patens WUSCHEL-related homeobox 13-like (PpWOX13) genes, homologs of stem cell regulators in flowering plants, were transiently up-regulated during moss stem cell formation. PpWOX13 genes were found upregulated during stem cell formation in detached leaves (Sakakibara et al 2014). We found that AhWOX13A and AhWOX13B expressed strongly in peanut root and were upregulated in RAM enriched region compared with root without RAM.…”

Section: Discussionmentioning

confidence: 67%

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Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut

Wang

Cui

et al. 2015

Dev Genes Evol

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WUSCHEL-related homeobox (WOX) genes play key roles in plant stem cell maintenance and development. WOX genes showed specific expression patterns which are important for their functions. WOX13 subfamily genes as the ancestor genes of this family were less studied in the past. In this study, we cloned three Arachis hypogaea (peanut) WOX13 (AhWOX13) subfamily genes from peanut: WOX13A and WOX13B1, 2. WOX13B1 encoded a same protein as WOX13B2, and there were only two-base difference between these two genes. Differential expression patterns were observed for these three AhWOX13 subfamily genes in different tissues and developmental stages. Phylogenic trees analysis showed that these AhWOX13 subfamily genes were the most conserved WOX genes and belonged to the ancient clade of WOX family. This was also supported by the conserved motif analysis. Selective pressure analysis showed that the WOX family genes mainly underwent weak purifying selection (ω = 0.58097), while many positive mutations accumulated during the evolution history. Under the purifying selection, gene duplication event and loss of duplicated gene play important roles in the expansion and evolution of WOX family.

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Section: Discussionmentioning

confidence: 67%

“…Only the members of the ancient/basal clade had a motif of 41 aa which contained the N-terminal domain (Sakakibara et al 2014), we named it ancient/basal motif. Ancient/basal motif was located in the N-terminal region of the HD domain ( Fig.…”

Section: Phylogenetic and Conservation Motifs Analyses Of Wox Family mentioning

confidence: 99%

Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut

Wang

Cui

et al. 2015

Dev Genes Evol

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“…Scale bars: a 6 μm, b 50 μm, c 1 mm, d 500 μm, e 200 μm, f 100 μm This high regeneration capacity also supports the cultivation of moss tissue in liquid medium using weekly disruption cycles and the easy regeneration of plants from protoplasts. The molecular mechanisms underlying this pluripotency have been investigated and unraveled several key players including a basic helix loop helix transcription factor and two WUSCHEL-related homeobox 13-like genes (Busch et al 2013;Nishiyama et al 2012;Sakakibara et al 2014). …”

Section: Mosses Are Haploid Dominantmentioning

confidence: 99%

Can mosses serve as model organisms for forest research?

Müller

Gütle

Jacquot

et al. 2016

Annals of Forest Science

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& Key message Based on their impact on many ecosystems, we review the relevance of mosses in research regarding stress tolerance, metabolism, and cell biology. We introduce the potential use of mosses as complementary model systems in molecular forest research, with an emphasis on the most developed model moss Physcomitrella patens. & Context and aims Mosses are important components of several ecosystems. The moss P. patens is a well-established nonvascular model plant with a high amenability to molecular biology techniques and was designated as a JGI plant flagship genome. In this review, we will provide an introduction to moss research and highlight the characteristics of P. patens and other mosses as a potential complementary model system for forest research. & Methods Starting with an introduction into general moss biology, we summarize the knowledge about moss physiology and differences to seed plants. We provide an overview of the current research areas utilizing mosses, pinpointing potential links to tree biology. To complement literature review, we discuss moss advantages and available resources regarding molecular biology techniques. & Results and conclusion During the last decade, many fundamental processes and cell mechanisms have been studied in mosses and seed plants, increasing our knowledge of plant evolution. Additionally, moss-specific mechanisms of stress tolerance are under investigation to understand their resilience in ecosystems. Thus, using the advantages of model mosses such as P. patens is of high interest for various research approaches, including stress tolerance, organelle biology, cell polarity, and secondary metabolism.

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“…In many plants, including Arabidopsis thaliana, Fucus (brown algae), Physcomitrella patens (moss), or rice (Brawley et al 1977;He et al 2007;Sato et al 2010;Sakakibara et al 2014), the zygote divides asymmetrically. In Arabidopsis, this division yields a small apical daughter cell that forms the embryo and a large basal daughter cell that will give rise mostly to the extraembryonic suspensor (Mansfield and Briarty 1991).…”

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confidence: 99%

Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote

et al. 2017

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In many plants, the asymmetric division of the zygote sets up the apical-basal axis of the embryo. Unlike animals, plant zygotes are transcriptionally active, implying that plants have evolved specific mechanisms to control transcriptional activation of patterning genes in the zygote. In Arabidopsis, two pathways have been found to regulate zygote asymmetry: YODA (YDA) mitogen-activated protein kinase (MAPK) signaling, which is potentiated by sperm-delivered mRNA of the SHORT SUSPENSOR (SSP) membrane protein, and up-regulation of the patterning gene WOX8 by the WRKY2 transcription factor. How SSP/YDA signaling is transduced into the nucleus and how these pathways are integrated have remained elusive. Here we show that paternal SSP/YDA signaling directly phosphorylates WRKY2, which in turn leads to the up-regulation of WOX8 transcription in the zygote. We further discovered the transcription factors HOMEODOMAIN GLABROUS11/12 (HDG11/12) as maternal regulators of zygote asymmetry that also directly regulate WOX8 transcription. Our results reveal a framework of how maternal and paternal factors are integrated in the zygote to regulate embryo patterning.

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WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens

Cited by 125 publications

References 65 publications

Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut

Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut

Can mosses serve as model organisms for forest research?

Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote

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