<i>Wolbachia</i>modulates Chikungunya replication in<i>Aedes albopictus</i>

Mousson, Laurence; Martin, Estelle; Zouache, Karima; Madec, Yoann; Mavingui, Patrick; Failloux, Anna‐Bella

doi:10.1111/j.1365-294x.2010.04606.x

Cited by 89 publications

(104 citation statements)

References 53 publications

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“…However, Plasmodium parasites may modulate Wolbachia densities, leading to a more complex interaction. In Aedes albopictus, which is naturally infected with two Wolbachia strains, wAlbA and wAlbB, Chikungunya virus infection modulates bacterium levels, lowering the density of both strains (28,37).…”

Section: Discussionmentioning

confidence: 99%

Wolbachia Strain wAlbB Enhances Infection by the Rodent Malaria Parasite Plasmodium berghei in Anopheles gambiae Mosquitoes

Hughes

Vega-Rodríguez

Xue

et al. 2012

Appl Environ Microbiol

112

116

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Wolbachia, a common bacterial endosymbiont of insects, has been shown to protect its hosts against a wide range of pathogens. However, not all strains exert a protective effect on their host. Here we assess the effects of two divergent Wolbachia strains, wAlbB from Aedes albopictus and wMelPop from Drosophila melanogaster, on the vector competence of Anopheles gambiae challenged with Plasmodium berghei. We show that the wAlbB strain significantly increases P. berghei oocyst levels in the mosquito midgut while wMelPop modestly suppresses oocyst levels. The wAlbB strain is avirulent to mosquitoes while wMelPop is moderately virulent to mosquitoes pre-blood meal and highly virulent after mosquitoes have fed on mice. These various effects on P. berghei levels suggest that Wolbachia strains differ in their interactions with the host and/or pathogen, and these differences could be used to dissect the molecular mechanisms that cause interference of pathogen development in mosquitoes.

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Section: Discussionmentioning

confidence: 99%

Wolbachia Strain wAlbB Enhances Infection by the Rodent Malaria Parasite Plasmodium berghei in Anopheles gambiae Mosquitoes

Hughes

Vega-Rodríguez

Xue

et al. 2012

Appl Environ Microbiol

112

116

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“…We subsequently found that the Yolk-Gal4 D. melanogaster flies used for these experiments were positive for a strain of W. pipientis that is closely related to wMel (Fig. 4A and data not shown) and known to increase the resistance to infection by several arboviruses (6,30,36,53,69). We therefore used tetracycline treatment to generate a W. pipientis-free line of D. melanogaster (Yolk-T) from the original, untreated Yolk-Gal4 stock (Yolk-NT).…”

Section: Methodsmentioning

confidence: 99%

“…More recently, it has become increasingly common to use D. melanogaster to study insect-pathogen interactions (17,65). For instance, the bacterial endosymbiont Wolbachia pipientis (wMel strain) of D. melanogaster increases resistance to infection by several RNA viruses, including many mosquito-transmitted arboviruses that are pathogenic in humans (6,30,36,53,69).…”

Section: B Luetongue Virus (Btv) Is An Arbovirus Belonging To the Genusmentioning

confidence: 99%

Drosophila melanogaster as a Model Organism for Bluetongue Virus Replication and Tropism

Shaw

Veronesi

Maurin

et al. 2012

J Virol

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c Bluetongue virus (BTV) is the etiological agent of bluetongue (BT), a hemorrhagic disease of ruminants that can cause high levels of morbidity and mortality. BTV is an arbovirus transmitted between its ruminant hosts by Culicoides biting midges (Diptera: Ceratopogonidae). Recently, Europe has experienced some of the largest BT outbreaks ever recorded, including areas with no known history of the disease, leading to unprecedented economic and animal welfare issues. The current lack of genomic resources and genetic tools for Culicoides restricts any detailed study of the mechanisms involved in the virus-insect interactions. In contrast, the genome of the fruit fly (Drosophila melanogaster) has been successfully sequenced, and it is used extensively as a model of molecular pathways due to the existence of powerful genetic technology. In this study, D. melanogaster is investigated as a model for the replication and tropism of BTV. Using reverse genetics, a modified BTV-1 that expresses the fluorescent mCherry protein fused to the viral nonstructural protein NS3 (BTV-1/NS3mCherry) was generated. We demonstrate that BTV-1/NS3mCherry is not only replication competent as it retains many characteristics of the wild-type virus but also replicates efficiently in D. melanogaster after removal of the bacterial endosymbiont Wolbachia pipientis by antibiotic treatment. Furthermore, confocal microscopy shows that the tissue tropism of BTV-1/NS3mCherry in D. melanogaster resembles that described previously for BTV in Culicoides. Overall, the data presented in this study demonstrate the feasibility of using D. melanogaster as a genetic model to investigate BTV-insect interactions that cannot be otherwise addressed in vector species.

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“…De plus, chez Ae. albopictus, qui est naturellement infecté par Wolbachia, la bactérie ne semble pas affecter la réplication du virus chikungunya [28]. L'effet de priming observé chez Ae.…”

Section: Des Symbiotes Obligatoires Comme Cible Thérapeutique : Exempunclassified

Les bactéries symbiotiques d’arthropodes et de nématodes

Vavre

Mavingui

2011

Med Sci (Paris)

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> Les arthropodes et les nématodes sont des acteurs importants de la santé humaine en étant soit des vecteurs de pathogènes (bactéries, champignons, protozoaires, virus), soit eux-mê-mes des parasites. Si la lutte contre les maladies infectieuses repose essentiellement sur l'utilisation de la vaccination ou de traitements curatifs chez l'homme, la réduction des populations de vecteurs ou la limitation de leur compétence vectorielle constitue des stratégies alternatives de contrôle. Dans ce domaine, des travaux récents sur les bactéries symbiotiques hébergées par les arthropodes et les nématodes permettent d'envisager, et même de mettre en place, des stratégies originales de contrôle. Des symbiotes qui font partie du patrimoine génétique des individusLes arthropodes et les nématodes sont fréquemment infectés par des bactéries avec lesquelles ils entretiennent une relation durable. Ces symbiotes sont pour la plupart intracellulaires (raison pour laquelle on les qualifie fréquemment d'endosymbiotes). Ils infectent notamment les cellules reproductrices comme les ovocytes, ce qui assure leur transmission de mère à descendants. Les mâles, dont les spermatozoïdes sont pourvus d'un cytoplasme très réduit, peuvent également être porteurs, mais ils ne sont en aucun cas transmetteurs directs des symbiotes. Cette hérédité maternelle est identique à celle des mitochondries, qui ne sont rien d'autre que des symbiotes intégrés. En présence de ces symbiotes, le génotype des individus porteurs est donc composé du génome nucléaire, du génome mitochondrial et du génome de leur(s) symbiote(s).Peut-on comprendre la biologie des organismes sans considérer leurs symbiotes ? Diverses études, conduites notamment chez les insectes, indiquent que la majorité des espèces hébergent des endosymbiotes. Par exem-

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Wolbachiamodulates Chikungunya replication inAedes albopictus

Cited by 89 publications

References 53 publications

Wolbachia Strain wAlbB Enhances Infection by the Rodent Malaria Parasite Plasmodium berghei in Anopheles gambiae Mosquitoes

Wolbachia Strain wAlbB Enhances Infection by the Rodent Malaria Parasite Plasmodium berghei in Anopheles gambiae Mosquitoes

Drosophila melanogaster as a Model Organism for Bluetongue Virus Replication and Tropism

Les bactéries symbiotiques d’arthropodes et de nématodes

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