<i>Watermelon chlorotic stunt virus</i> from the Sudan and Iran: Sequence Comparisons and Identification of a Whitefly-Transmission Determinant

Kheyr-Pour, A.; Bananej, K.; Dafalla, Gasim; Caciagli, P.; Noris, Emanuela; Ahoonmanesh, A.; Lecoq, Hervé; Gronenborn, Bruno

doi:10.1094/phyto.2000.90.6.629

Cited by 106 publications

(91 citation statements)

References 48 publications

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“…3b) showed that the BotGn-1 belonged to the ToLCNDV cluster. Symptoms of the disease are similar to watermelon leaf curl disease described from Pakistan [18] and watermelon chlorotic curly stunt disease described from Iran and Sudan [5,13] which also result in mottling, curling of leaves and stunting of plants. The virus associated with the CCS disease of bottle gourd did not infect watermelon by whitefly inoculation, indicating differences between the viruses or their strains causing these diseases.…”

Section: Host Rangesupporting

confidence: 53%

“…Since then, the diseases caused by begomoviruses have been reported in bitter gourd, chayote, cucumber, muskmelon, pumpkin and sponge gourd [17,20,22,30,34]. Many different begomoviruses infecting cucurbits have been reported from other countries such as Squash leaf curl Yunnan virus from China [39], Melon chlorotic leaf curl virus from Guatemala [3] Chayote yellow mosaic virus from Nigeria [33], ToL-CNDV from Thailand [26,27], Watermelon chlorotic curly stunt virus from Sudan and Iran [13], Squash leaf curl Philippines virus from the Philippines [14], Cucurbit leaf curl virus [2], Cucurbit leaf crumple virus [9], Squash leaf curl virus [8,16] and Squash mild leaf curl virus [4] from USA, and Luffa yellow mosaic virus from Vietnam [24].…”

Section: Introductionmentioning

confidence: 99%

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Chlorotic Curly Stunt: A Severe Begomovirus Disease of Bottle Gourd in Northern India

et al. 2010

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Bottle gourd (Lagenaria siceraria) an important vegetable crop in India was observed to be affected by a chlorotic curly stunt disease (CCSD) during [2003][2004][2005][2006] in the vegetable growing areas of Delhi and adjoining state of Haryana. The affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. Incidence of the disease varied from 4.7 to 36%. The disease could be easily transmitted by whitefly, Bemisia tabaci but not by sap. The causal virus was found to be a Begomovirus on the basis of whitefly transmission and sequence identity of putative coat protein (CP) and replication initiator protein (Rep) genes. The virus was transmitted to Cucumis sativus, Luffa acutangula, L. cylndrica, Lycopersicon esculentum, Nicotiana tabacum and Praecitrullus fistulosus but not to Citrullus lunatus, Cucumis melo, Cucurbita moschata and Vigna unguiculata. The N-terminal 60 amino acids of CP of the virus had 100% sequence identity with all the isolates of Tomato leaf curl New Delhi virus (ToLCNDV) and two isolates of Squash leaf curl China virus (SLCCV). The full length amino acid sequence of the CP and Rep genes had 100% similarity with ToLCNDVSvr and -Luffa isolates. The phylogenetic analysis showed that the virus associated with CCSD of bottle gourd belongs to ToLCNDV cluster of the begomoviruses. This is the first record of emergence of a Begomovirus associated severe disease in bottle gourd in India.

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Section: Host Rangesupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Chlorotic Curly Stunt: A Severe Begomovirus Disease of Bottle Gourd in Northern India

et al. 2010

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“…The single CP possesses a molecular mass of 30.1 kDa and a calculated pI of 10.0. Each geminate particle encapsidates one circular DNA molecule so that two virions are needed for infection in case of bipartite geminiviruses.Encapsidating the viral genome, the geminivirus CP is important for insect transmission (Briddon et al, 1990; Höfer et al, 1997; Höhnle et al, 2001;Kheyr-Pour et al, 2000; Noris et al, 1998) and transport of the viral DNA in and out of the nucleus (Kotlizky et al, 2000;Kunik et al, 1998Kunik et al, , 1999 Liu et al, 1999;Unseld et al, 2001Unseld et al, , 2004. For the ACMV CP, three regions have been determined that mediate nuclear import (Unseld et al, 2001), whereby the central region is not only important for nuclear import but also for export.…”

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confidence: 99%

Disassembly of African cassava mosaic virus

Kittelmann

Jeske

2008

Journal of General Virology

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The plant-infecting geminiviruses encapsidate their single-stranded DNA genome in characteristic twinned particles that are unique among viruses. These particles are formed by joining two incomplete T51 icosahedra. African cassava mosaic virions were purified by densitygradient centrifugation from infected Nicotiana benthamiana plants and analysed for their stability with respect to pH changes and heat treatment by using electron microscopy. Negative staining and rotary shadowing revealed stable virions as well as isolated capsomeres between pH 4.0 and 8.5. At pH 9.0 and above, particles disintegrated, whereas they mainly aggregated at a pH below 6.0. Heating the preparations to 55 6C and above resulted in the complete loss of any discernible structure. A low proportion (approx. 10 %) of particles ejected their DNA within the pH range of 6.0-8.5. Most virions released their DNA at the top (15.9 %) or the shoulder (71.4 %) of the twin particles and only 12.7 % at the waist. Compared with the expected numbers of pentameric capsomeres at the top (9 %), the shoulder (45.5 %) or the waist (45.5 %), the results revealed a preferential DNA release from the top and shoulder of the geminate particle. INTRODUCTIONThe small plant pathogenic geminiviruses are distributed all over the world and have caused severe economic damages in tropical and subtropical countries (Moffat, 1999). African cassava mosaic virus (ACMV) is the most prevalent pathogen of cassava in Africa (Fauquet & Fargette, 1990); it belongs to the genus Begomovirus (Stanley et al., 2005), and is transmitted by the whitefly Bemisia tabaci (Gennadius). Its bipartite genome consists of two circular single-stranded DNA molecules (DNA A and DNA B). The three-dimensional structure of the geminate (twin) ACMV particles has been resolved by cryo-electron microscopy (EM) and image reconstruction (Böttcher et al., 2004). The two incomplete T51 icosahedra of the virion are formed by 110 copies of a single capsid protein (CP) with pentameric capsomere organization. Each half of the geminate particle is built up by 11 pentameric capsomeres, one pentamer missing to complete a T51 icosahedron. The two halves are joined at the place of the missing capsomeres, forming the waist of the particle (see Fig. 7). The two capsomeres at both ends of the particle with strict fivefold symmetry will be called the 'top capsomeres', the adjacent five capsomeres on each side of the virion with local fivefold symmetry will be called the 'shoulder capsomeres' and the five remaining capsomeres at the waist will be called the 'waist capsomeres' (see Fig. 7). The single CP possesses a molecular mass of 30.1 kDa and a calculated pI of 10.0. Each geminate particle encapsidates one circular DNA molecule so that two virions are needed for infection in case of bipartite geminiviruses.Encapsidating the viral genome, the geminivirus CP is important for insect transmission (Briddon et al., 1990; Höfer et al., 1997; Höhnle et al., 2001;Kheyr-Pour et al., 2000; Noris et al., 1998) and transport of the...

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“…All cucurbit-infecting begomoviruses reported at present have a bipartite genome (18). In the Eastern Hemisphere, Watermelon chlorotic stunt virus has a native range spanning Sudan, southern Arabia, and Iran (23,33). (25) occurs in China, the Philippines (34), and Vietnam (34), whereas Squash leaf curl Yunnan virus has been reported only in southern China (54).…”

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confidence: 99%

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002. The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at ϳ90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica. The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade, which is endemic in the southwestern United States and Mexico. Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms, providing definitive evidence of causality. MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae. The potential for interspecific reassortment was examined for MCLCuV and its closest relatives, including the bean-restricted Bean calico mosaic virus (BCaMV), and three other cucurbit-infecting species, Cucurbit leaf crumple virus (CuLCrV), SLCV, and SMLCV. The cucurbit viruses have distinct but overlapping host ranges. All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components. Surprisingly, only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean, even though it is a host of all of the "wild-type" (parent) viruses. The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses. In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species. Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses, strongly implicating adaptive modulation of virulence. This is the first illustration of reassortment resulting in the host range expansion of a host-restricted begomovirus.

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Watermelon chlorotic stunt virus from the Sudan and Iran: Sequence Comparisons and Identification of a Whitefly-Transmission Determinant

Cited by 106 publications

References 48 publications

Chlorotic Curly Stunt: A Severe Begomovirus Disease of Bottle Gourd in Northern India

Chlorotic Curly Stunt: A Severe Begomovirus Disease of Bottle Gourd in Northern India

Disassembly of African cassava mosaic virus

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

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