<i>Vibrio alginolyticus</i>infections in the USA, 1988–2012

Slifka, Kara M. Jacobs; Newton, Anna E.; Mahon, Barbara E.

doi:10.1017/s0950268817000140

Cited by 84 publications

(52 citation statements)

References 30 publications

(43 reference statements)

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“…Regarding virulence-associated factors, hemolytic activity has been reported [12]. Also vibrios are included in the marine microbial community and they are able to cause severe or mild gastroenteritis in humans [17,18]. Most of the species belonging to the genus Vibrio are harmless to humans except for the "big four" representing by V. cholerae, V. parahaemolyticus, V. vulnificus and V. alginolyticus [19].…”

Section: Introductionmentioning

confidence: 99%

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

et al. 2020

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Antimicrobial resistance is a major public health concern restricted not only to healthcare settings but also to veterinary and environmental ones. In this study, we analyzed, by whole genome sequencing (WGS) the resistome, mobilome and virulome of 12 multidrug-resistant (MDR) marine strains belonging to Shewanellaceae and Vibrionaceae families collected at aquaculture centers in Italy. The results evidenced the presence of several resistance mechanisms including enzyme and efflux pump systems conferring resistance to beta-lactams, quinolones, tetracyclines, macrolides, polymyxins, chloramphenicol, fosfomycin, erythromycin, detergents and heavy metals. Mobilome analysis did not find circular elements but class I integrons, integrative and conjugative element (ICE) associated modules, prophages and different insertion sequence (IS) family transposases. These mobile genetic elements (MGEs) are usually present in other aquatic bacteria but also in Enterobacteriaceae suggesting their transferability among autochthonous and allochthonous bacteria of the resilient microbiota. Regarding the presence of virulence factors, hemolytic activity was detected both in the Shewanella algae and in Vibrio spp. strains. To conclude, these data indicate the role as a reservoir of resistance and virulence genes in the environment of the aquatic microbiota present in the examined Italian fish farms that potentially might be transferred to bacteria of medical interest.

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Section: Introductionmentioning

confidence: 99%

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

et al. 2020

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“…An analysis of both COVIS data and data from the 10-state Foodborne Diseases Active Surveillance Network (FoodNet) from 1996 to 2010 showed a dramatic increase in the incidence of V. alginolyticus infection in both systems [2]. Using data from 1988 to 2012, Newton et al also categorized infections using specimen source and exposure history, analyzed case characteristics, and calculated the incidence rates using the US Census Bureau data [11]. The reported incidence of infections increased 12-fold over the study period, although the extent of diagnostic or surveillance bias was unclear.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms underlying the virulence regulation of new Vibrio alginolyticus ncRNA Vvrr1 with a comparative proteomic analysis

Zuo

Zhao

et al. 2019

Emerging Microbes & Infections

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The incidence of Vibrio alginolyticus infections has increased in recent years due to the influence of climate change and rising sea temperature. Vibrio virulence regulatory RNA 1 (Vvrr1) is a newly found noncoding RNA (ncRNA) predicted to be closely related to the adhesion ability of V. alginolyticus based on the previous RNA-seq. In this study, the target genes of Vvrr1 were fully screened and verified by constructing Vvrr1-overexpressing strains and using the proteome sequencing technology. Pyruvate kinase I (pykF) gene was predicted to be a chief target gene of Vvrr1 involved in virulence regulation. The adhesion ability, biofilm formation and virulence were significantly reduced in the Vvrr1-overexpressing and the pykFsilenced strain compared with the wild strains. Similar to the overexpression of Vvrr1, the silencing of pykF also reduced the expression level of virulence genes, such as ndk, eno, sdhB, glpF, and cysH. Meanwhile, by constructing the "pykF-GFP" fusion expression plasmid and using the GFP reporter gene analysis in Escherichia coli, the fluorescence intensity of the strain containing Vvrr1 whole ncRNA sequence vector was found to be significantly weakened. These indicated that Vvrr1 participated in the virulence regulation mechanism of V. alginolyticus by interacting with the virulence gene pykF.

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“…Fish fillets, shrimp, and oysters were the types of food that showed more species richness, and the most abundant isolated species in the seafood samples was V. alginolyticus, with the exception of oysters and clamshells, in which the most abundant species were V. vulnificus and V. parahaemolyticus, respectively. Vibrio alginolyticus is the species with the highest prevalence in Sri Lanka (34.6%) (Koralage et al, 2012), Germany (35.6%) (Vu, Alther, & Huehn, 2018), and Ecuador (50.2%) (Sperling et al, 2015); this species has gained importance as a causative agent of gastrointesti-nal diseases, which are usually incorrectly diagnosed (Jacobs Slifka, Newton, & Mahon, 2017;Oh et al, 2011).…”

Section: Prevalence Of Vibrio Sppmentioning

confidence: 99%

A new classification criterion for the biofilm formation index: A study of the biofilm dynamics of pathogenic Vibrio species isolated from seafood and food contact surfaces

Lucero-Mejía

Romero-Gómez

Hernández‐Iturriaga

2020

Journal of Food Science

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The bacterial biofilm formation index (BFI) is measured by a microtiter plate assay, and it is typically performed at 72 hr. However, the dynamics of biopolymer formation change during this incubation period. The aims of this study were to follow the biofilm formation dynamics of Vibrio strains isolated from samples of seafood and food contact surfaces (FCS) and to propose a new BFI classification criterion. Samples from seafood (136) and FCS (14) were collected from retail markets in Queretaro, Mexico. The presence of Vibrio spp. was determined, the strains were isolated, and the six major pathogenic species (V. cholerae, V. alginolyticus, V. fluvialis, V. parahaemolyticus, V. vulnificus, V. mimicus) were identified by PCR. The BFI of the isolates was determined by the microtiter plate method. Fifty‐one strains were isolated and identified as V. alginolytivcus (25), V. vulnificus (12), V. cholerae (7), V. parahaemolyticus (6), and V. mimicus (1). A quantitative classification criterion of biofilm formation was proposed based on the following factors: BFI dynamics (no formation, continuous increase, and increase followed by decrease), time of maximum BFI (early: 24 hr; late: 48 to 72 hr), and degree of BFI (none, weak, moderate, and strong). A numerical value was assigned to each factor to correlate the resulting BFI profile with a risk level. Thirteen BFI profiles were observed, having risk level values from 0 to 10. Vibrio alginolyticus, V. cholerae, and V. vulnificus showed the highest BFI profile diversities, which included the riskiest profiles. The proposed BFI criterion describes the dynamics of bacterial biopolymer formation and associates them with the possible risk implications. Practical Application In food processing environments, the presence of bacterial biofilms that could include foodborne pathogens might favor cross‐contamination due to direct contact or biofilm dispersal into food products. The new quantitative classification criterion for biofilm formation considers their production dynamics over time, the biofilm quantity, and the level of biofilm dispersal. These characteristics are represented by a numerical value that reflects the level of risk associated with the presence of a biofilm‐producing strain on a food contact surface.

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Vibrio alginolyticusinfections in the USA, 1988–2012

Cited by 84 publications

References 30 publications

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

Resistome, Mobilome and Virulome Analysis of Shewanella algae and Vibrio spp. Strains Isolated in Italian Aquaculture Centers

Mechanisms underlying the virulence regulation of new Vibrio alginolyticus ncRNA Vvrr1 with a comparative proteomic analysis

A new classification criterion for the biofilm formation index: A study of the biofilm dynamics of pathogenic Vibrio species isolated from seafood and food contact surfaces

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