<i>Vernonia</i> DGATs increase accumulation of epoxy fatty acids in oil

Li, Runzhi; Yu, Keshun; Hatanaka, Tomoko; Hildebrand, David F.

doi:10.1111/j.1467-7652.2009.00476.x

Cited by 105 publications

(102 citation statements)

References 37 publications

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“…VfDGAT2 was highly expressed only in seeds, with uniformly low levels elsewhere, except for slightly higher expression in petals and pistils, suggesting that VfDGAT2 is expressed mainly in reproductive rather than vegetative organs. These results are similar to those for the expression of DGAT2 in Arabidopsis (Hobbs et al, 1999), R. communis (Kroon et al, 2006), and V. galamensis (Li et al, 2010b).…”

Section: Discussionsupporting

confidence: 77%

“…In addition, DGAT2 was reported to be a potential contributor to the desired accumulation of TAG (Shockey et al, 2006;Cahoon et al, 2007). Li et al (2010b) showed that both VgDGAT1 and VgDGAT2 isolated from V. galamensis could increase the accumulation of epoxy fatty acids in soybean oil. Similarly, RcDGAT2 cloned from castor bean (R. communis) could increase hydroxy fatty acid content from 17 to nearly 30% in transgenic Arabidopsis (Burgal et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…During the course of TAG synthesis, diacylglycerol acyltransferases (DGATs) catalyze the final and most committed step by transferring an acyl group from acyl-coenzyme A to diacylglycerol (Bouvier-Navé et al, 2000). Three distinct family members, DGAT1, DGAT2, and DGAT3, have been identified in several species such as Olea europaea (Banilas et al, 2011), Jatropha curcas (Xu et al, 2011), Ricinus communis (Cagliari et al, 2010), and Vernonia galamensis (Li et al, 2010b;Li et al, 2012), etc. DGAT may play an important role in oil accumulation in seeds (Zhang et al, 2005;Chen et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Expression analysis of VfDGAT2 in various tissues of the tung tree and in transgenic yeast

Cui

Chen²,

Han³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The tung tree (Vernicia fordii Hemsl.; Vf) has great potential as an industrial crop owning to its seed oil that has multiple uses. Diacylglycerol acyltransferases (DGATs) catalyze the last and most committed step of triacylglycerol (TAG) biosynthesis. In order to examine the physiological role of the VfDGAT2 gene in the tung tree, we characterized its expression profiles in different tung tissues/ organs and seeds at different developmental stages. Oil content and α-eleostearic acid production during seed development were also examined. Expression studies showed that VfDGAT2 was expressed in all tissues tested, with the highest expression in developing seeds where the expression was about 19-fold more than that in leaves. VfDGAT2 showed temporal-specific expression during seed development and maturation. Notably, the expression of VfDGAT2 in developing seeds was found to be consistent with tung oil accumulation and α-eleostearic acid production. The expression level of VfDGAT2 was lower in the early stages of oil accumulation and α-eleostearic acid biosynthesis, rapidly increased during the peak periods of fatty acid synthesis in August, and then decreased during completion of the accumulation period at the end of September. When the VfDGAT2 gene was transferred to the oleaginous yeast Rhodotorula glutinis, its expression was detected along with fatty acid products. The results showed that VfDGAT2 was highly expressed in transgenic yeast clones, and the total fatty acid content in one of these clones, VfDGAT2-3, was 7.8-fold more than that in the control, indicating that VfDGAT2 contributed to fatty acid accumulation into TAG and might be a target gene for improving tung oil composition through genetic engineering.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression analysis of VfDGAT2 in various tissues of the tung tree and in transgenic yeast

Cui

Chen²,

Han³

et al. 2013

Genet. Mol. Res.

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“…There, acyl-CoA: diacylglycerol acyltransferase (DGAT; EC 2.3.1.20) transfers an acyl-CoA to the sn-3 position of sn-1, 2-diacylglycerol (DAG) and plays an essential role in controlling TAG assembly (Shockey et al 2006). At least two classes of genes, DGAT1 and DGAT2, encoding the DGAT enzymes have been identified in diverse plants (Zou et al 1999;Giannoulia et al 2000;Lardizabal et al 2001;Nykiforuk et al 2002;He et al 2004;Yu et al 2008;Li et al 2010a). Results suggest that DGAT1 and DGAT2 are ubiquitous in plants.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, the DGAT1s significantly regulate TAG accumulation in maize (Zheng et al 2008), Tropaeolum majus L. (Xu et al 2008) and soybean (Li et al 2010b). Further, studies have shown that the DGAT2s play a critical role in the accumulation of unusual FAs (such as epoxy and hydroxy fatty acids) in castor bean (Kroon et al 2006), tung tree (Shockey et al 2006) and Vernonia (Li et al 2010a). In addition, temporal and spatial expression patterns of DGAT1s and DGAT2s among organs or in different stages of seed development often display obvious differences (Li et al 2010b;Banilas et al 2011;Xu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

Yang

Wang

et al. 2014

Functional Plant Biol.

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Abstract. Diacylglycerol acyltransferases (DGATs) catalyse the final step of triacylglycerol (TAG) biosynthesis of the Kennedy pathway, and play a critical role during TAG accumulation in developing oleaginous seeds. In this study, the molecular cloning and characterisation of two DGAT genes, JcDGAT1 and JcDGAT2, from jatropha (Jatropha curcas L., a potential biodiesel plant) is presented. Using heterogonous overexpression techniques, both JcDGAT1 and JcDGAT2 were able to restore TAG biosynthesis in a yeast mutant H1246 strain, and enhance the quantity of TAG biosynthesis by 16.6 and 14.3%, respectively, in strain INVSc1. In transgenic tobacco, overexpression of JcDGAT1 and JcDGAT2 resulted in an increase in seed oil content of, respectively, 32.8 and 31.8%. Further, the functional divergence of JcDGAT1 and JcDGAT2 in TAG biosynthesis was demonstrated by comparing the fatty acid compositions in both the transgenic yeast and tobacco systems. In particular, JcDGAT2 incorporated a 2.5-fold higher linoleic acid content into TAG than JcDGAT1 in transgenic yeast and exhibited a significant linoleic acid substrate preference in both yeast and tobacco. This study provides new insights in understanding the molecular mechanisms of DGAT genes underlying the biosynthesis of linoleic acids and TAG in plants.

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Transgenic Oils

Durrett¹

2020

Bailey's Industrial Oil and Fat Products

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Genetic engineering (GE) technology offers a powerful tool for developing a broad range of oils with specialized compositions useful for different food and industrial applications. Such oils could contain modified ratios of endogenous fatty acids, or even unusual fatty acids and lipids, produced through the transgenic expression of biosynthetic genes from other species. However, GE technology has mostly been limited to crop input traits such as herbicide tolerance and insect resistance. As this article will discuss, only recently have high oleic acid soybeans with transgenically modified oil compositions been grown on a commercial basis. For the most part, the synthesis of unusual fatty acids with useful functional groups only results in the accumulation of low levels of the desired product in the transgenic seed. The continually improving knowledge of fatty acid biosynthesis and triacylglycerol assembly in oilseeds will be explored. This knowledge, combined with new systems and synthetic biology approaches, promises to overcome biological bottlenecks limiting the production of unusual fatty acids in transgenic oilseeds. This article will also describe the regulatory and economic barriers that likely present significant barriers to the widespread adoption of novel oils with altered output traits. As discussed, new business models for the commercial production of specialized industrial oils will therefore need to be considered.

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Vernonia DGATs increase accumulation of epoxy fatty acids in oil

Cited by 105 publications

References 37 publications

Expression analysis of VfDGAT2 in various tissues of the tung tree and in transgenic yeast

Expression analysis of VfDGAT2 in various tissues of the tung tree and in transgenic yeast

Characterisation of DGAT1 and DGAT2 from Jatropha curcas and their functions in storage lipid biosynthesis

Transgenic Oils

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