v‐myb avian myeloblastosis viral oncogene homolog expression is a potential molecular diagnostic marker for B‐cell acute lymphoblastic leukemia

Abstract

Background B‐cell acute lymphoblastic leukemia (B‐ALL) is the most commonly diagnosed childhood malignancy worldwide and is especially common in Mexico. Additionally, the number of cases has increased in recent years. Thus, it is very important to develop molecular strategies to diagnose leukemia. The aim of this study was to investigate MYB expression and to determine its impact on the diagnosis of B‐ALL. Methods We analyzed the B‐ALL gene expression profile by microarray data mining. Bioinformatics analysis was performed to identify the genes that are overexpressed in leukemia. We determined that MYB was highly expressed in leukemia. Then, we validated MYB expression in 70 patients with B‐ALL and in 16 healthy controls (HCs) using qRT‐PCR. The results were statistically analyzed using the Kolmogorov‐Smirnov Z test, Mann‐Whitney U test, receiver operating characteristic curves, and the Youden index. Results The microarrays showed that MYB was overexpressed in B‐ALL patients with a fold change of 57.8728 and a P value of 2.56−195. MYB expression showed great variability among the patients analyzed. However, compared to the HCs, the B‐ALL patients had a P value < .0001, an area under the curve of 0.813, and a Youden index of 1.46, indicating the statistical significance. Conclusion MYB expression in B‐ALL cells could be a potential molecular marker for childhood leukemia.

The Proliferating Cell Nuclear Antigen (PCNA) Transcript Variants as Potential Relapse Markers in B-Cell Acute Lymphoblastic Leukemia

“…We selected data from B-cell acute lymphoblastic leukemia (B-ALL), healthy control B cells (B Cell) and B-ALL cell lines (B-CL) ( Table 1 ). The microarray data were selected for quality control, and the microarray analysis was performed using Partek Genomics Suite version 6.6 according to previous studies [ 14 , 15 , 16 , 17 , 18 , 19 ]. The significant differences between B-ALL, B-CL, and B-Cell were obtained using ASANOVA with an FDR p value < 0.005.…”

Low Expression of BRCA1 as a Potential Relapse Predictor in B-Cell Acute Lymphoblastic Leukemia

“…In this work, we used microarray data from ArrayExpress and Gene Expression Omnibus. The data set was composed of 10 B-ALL patients from BM and 4 healthy controls from peripheral blonds,20 according to previous studies 21–23. Significantly, differentially expressed genes in B-ALL were analyzed via single-cell RNA-Seq using the expression profile of CD34+ cells from human healthy BM as a baseline for normal hematopoiesis 24.…”

“…The PCR products were separated in a 2% (g/v) agarose gel and stained with ethidium bromide. After the PCR products were run at 80 V for 45 minutes, the products were visualized in the FUSION FX imaging system (Vilber Lourmat), as previously reported 23…”

“…We used 1 mL of TRIzol reagent (Thermo Fisher Scientific), according to the manufacturer's instructions as in previous reports. 22,23 cDNA synthesis was performed using the RevertAid Reverse Transcriptase Kit (Thermo Fisher Scientific) using 1000 ng of total RNA, according to a previous study. 22,23 The cDNA was stored at −20 °C until use.…”

“…22,23 cDNA synthesis was performed using the RevertAid Reverse Transcriptase Kit (Thermo Fisher Scientific) using 1000 ng of total RNA, according to a previous study. 22,23 The cDNA was stored at −20 °C until use.…”