<i>Ureaplasma urealyticum</i> urease genes; use of a UGA tryptophan codon

Blanchard, Alain

doi:10.1111/j.1365-2958.1990.tb00636.x

Cited by 122 publications

(87 citation statements)

References 37 publications

(24 reference statements)

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“…We utilized oligonucleotide primers derived from the Ureaplasma urealyticum urease gene sequence (8), corresponding to nucleotides 1613-1635 ( U U I ; CAGATACAT-TAAACGAAGCAGG = sense sequence), 1837-1819 (UU2; GTGGTGACATACCATTAAC = antisense sequence), and 1701-1677 (UU3; CAGCACCTTCTG-TATGGTAAGCTG = antisense sequence). Additional primers specific for a DNA sequence common to all Chlamydia trachomatis serovars (9) were also utilized.…”

Section: Resultsmentioning

confidence: 99%

Molecular diagnosis of ureaplasma urealyticum septic arthritis in a patient with hypogammaglobulinemia

Lee

Ramanujam

Ware

et al. 1992

Arthritis & Rheumatism

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Objective. We report a hypogammaglobulinemic patient with a destructive oligoarticular arthritis, whose synovial fluid cultures were repeatedly sterile.Methods and Results. We identified a Ureaplasma ureulyticurn infection in his affected joints, using a polymerase chain reaction (PCR) assay. Conclusion. The PCR technique promises to be extremely valuable in the rapid and specific diagnosis of infectious arthritis.Ureaplasma urealyticum, a mycoplasma organism which commonly colonizes the human urogenital tract, has been described as the cause of septic arthritis in several patients with hypogammaglobulinemia (14). However, this organism is difficult to isolate in culture and requires special culture media. We report a hypogammaglobulinemic patient with a destructive oligoarticular arthritis, in whom microbiologic cultures were sterile, but in whom a polymerase chain reaction (PCR) assay revealed the presence of U urealyticurn.Analysis of T cell receptor transcripts present in the synovium indicated a polyclonal population of T cells responding to this infection. CASE REPORTThe patient, a 27-year-old black man with the a-thalassemia trait and dysgammaglobulinemia, was admitted to the Hospital of the University of Pennsylvania for evaluation of persistent pain and swelling in the wrists and right ankle. He had. been healthy until the age of 14, when he began to have recurrent bouts of pneumonia. Serum imrnunoglobulin measurement showed an IgM level of 9.25 gm/ liter (normal 0.7-2.10) and undetectable IgG and IgA. Immunoglobulin deficiency with increased IgM was diagnosed, and the patient was treated with intermittent intravenous immunoglobulin therapy.At age 21 he began to experience pain and swelling of the right ankle and right shoulder. Shoulder roentgenograms revealed a destructive osteolysis of subchondral bone in the right humeral head. Subsequently, the right shoulder was surgically fused. Cultures for bacteria, rnycobacteria, and fungi, from material obtained at surgery, were sterile, and results of assays for rheumatoid factor were negative. Three months later, a surgical fusion of the left ankle was at-

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Section: Resultsmentioning

confidence: 99%

Molecular diagnosis of ureaplasma urealyticum septic arthritis in a patient with hypogammaglobulinemia

Lee

Ramanujam

Ware

et al. 1992

Arthritis & Rheumatism

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“…2) [14]. DNA from all serotypes of UI urealyticum but not from M. bovis or P vulgaris was amplified.…”

Section: Suitability Of the Methods Investigated For The Detection Ofmentioning

confidence: 99%

Detection of Ureaplasma urealyticum in urine of patients with systemic lupus erythematosus and healthy individuals by culture and polymerase chain reaction

Runge¹,

Rykena²,

Wildhagen³

et al. 1997

Journal of Medical Microbiology

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A method was developed to detect Ureaplasma urealyticum in urine by the polymerase chain reaction (PCR). A 457-bp fragment of the urease gene of U. urealyticum was amplified by PCR. Before PCR, components disturbing the amplification had to be reduced. This was possible by diluting the urine 1 in 10 with distilled water and by the extraction of the U. urealyticum DNA. Urine specimens from 41 patients with systemic lupus erythematosus (SLE) and 21 healthy individuals were treated by the dilution method and investigated by PCR for U. urealyticum DNA. The results were compared with those obtained by culture and the detection rates of PCR and culture were found to be identical. Also there was no difference in the detection rates of U. urealyticum from urine of SLE patients and healthy individuals; 10 (24.4%) of the 41 urine specimens from SLE patients and five (23.8%) of the 21 urine specimens from healthy individuals gave positive results for U. urealyticum. The results of this study do not indicate a decisive role for U. urealyticum in SLE.

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“…pneumoniae genome. Together with many other mycoplasmas, M. pneumoniae uses the universal stop codon UGA as a tryptophan codon (Yamao et al, 1985;Blanchard, 1990). Thus, the P1 gene carries more UGA codons for tryptophan than UGG, the conventional tryptophan codon (Table 1).…”

Section: The P1 Operon Genes and Proteinsmentioning

confidence: 99%

“…Unfortunately, this approach encountered a serious problem. The usage by Mycoplasma, Ureaplasrna and Spiroplasma species of the universal stop codon UGA as a tryptophan codon (for references see Blanchard, 1990) hinders the expression in E. coli of mycoplasmal messages. This is particularly true for genes like P1 and MgPa, which carry more UGA than UGG codons (Table l), explaining the production of truncated rather than complete adhesin proteins in E. coli transfected with these genes (Trevino et al, 1986;Schaper et al, 1987).…”

Section: Immunogenicity Of Adhesins and Vaccine Engineeringmentioning

confidence: 99%