<i>Ureaplasma urealyticum</i>
            Continuous Ambulatory Peritoneal Dialysis-Associated Peritonitis Diagnosed by 16S rRNA Gene PCR

Yager, Jessica; Ford, Emily; Boas, Zachary P.; Haseley, Leah A.; Cookson, Brad T.; SenGupta, Dhruba J.; Fang, Ferric C.; Gottlieb, Geoffrey S.

doi:10.1128/jcm.01045-10

Cited by 14 publications

(6 citation statements)

References 12 publications

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“…For the seven cases with possible pathogens, case reports underline the plausibility of the PCR result, i.e., Pseudomonas aeruginosa from an HV sample in endocarditis (1,7,17), Ureaplasma urealyticum in a female patient with peritonitis and adnexitis (3,24), and Staphylococcus epidermidis from SF in an arthritis patient (9). The four further orthopedic samples yielded anaerobic, fastidious, or noncultivable organisms (i.e., a clostridial bacterium, Finegoldia magna, Granulicatella adiacens, and Tropheryma whipplei), each of which is a documented possible agent of arthritis (8, 10-12, 15, 18-20).…”

Section: Discussionmentioning

confidence: 99%

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

et al. 2012

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A new commercially available universal 16S and 18S rRNA gene PCR test, which is followed by sequence analysis of amplicons (SepsiTest), was evaluated for rapid identification of pathogens in the diagnosis of bone and joint infections. Eighty-three orthopedic samples and 21 specimens from other normally sterile body sites collected from 84 patients were analyzed in parallel by culture and PCR for detection of bacteria and fungi. Compared to culture, the diagnostic sensitivity and specificity of PCR were 88.5% and 83.5%, respectively. The detection rate of PCR (34.6%) was higher than that of bacterial culture (25.0%) as a consequence of the presence of fastidious and noncultivable species in samples and antibiotic treatment of patients. Thirteen culturenegative infections were identified by PCR, and PCR was able to detect culture-proven polymicrobial infections. On the other hand, three samples were culture positive but PCR negative. SepsiTest was demonstrated to be a valuable supplemental tool in the rapid detection of bacteria, especially for fastidious and noncultivable organisms, allowing earlier initiation of pathogenadapted therapy in patients with bone and joint infections.

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Section: Discussionmentioning

confidence: 99%

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

et al. 2012

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“…Species-level identification of bacterial isolates was achieved by 16S rRNA gene sequencing. Genomic DNA was extracted using a NucliSENS easyMag kit (bioMérieux, Marcy l'Etoile, France), and PCR amplification and DNA sequencing of the 16S rRNA genes were performed as described previously (30). Analysis with the BLAST program was used to search the NCBI database for each sequence obtained.…”

Section: Study Populationmentioning

confidence: 99%

Optimization of Periprosthetic Culture for Diagnosis of Propionibacterium acnes Prosthetic Joint Infection

et al. 2011

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Propionibacterium acnes is increasingly recognized as an important agent of prosthetic joint infection (PJI).However, the optimum culture conditions for recovery of this organism from PJI specimens have not been determined. By applying a prolonged 28-day culture incubation to all periprosthetic specimens received for bacterial culture from 198 revision arthroplasty procedures, we retrospectively determined that a 13-day culture incubation period is necessary for the recovery of P. acnes from patients with PJI. Incubation beyond this period was associated with increasing recovery of nondiagnostic isolates: 21.7% of P. acnes isolates believed to be clinically unimportant were recovered after 13 days of incubation. Importantly, a diagnosis of P. acnes PJI would have been missed in 29.4% of patients had extended culture incubation been applied only to anaerobic culture media. Although specimens from P. acnes PJIs were more commonly associated with the presence of >2 culture media positive for growth, acute inflammation (>5 neutrophils/high-power field) was observed in only 40% of patients with PJIs that had more than one specimen submitted for bacterial culture. These results support the need for a minimum culture incubation period of 13 days to be applied to both aerobic and anaerobic culture media for all periprosthetic specimens. Optimal recovery of infecting organisms from PJI specimens will be an important component in generating a universal definition for PJI due to indolent agents of infection, such as P. acnes.

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“…Molecular methods based on species-specific PCR or broad- range PCR for detecting the 16S rRNA gene followed by sequencing for species identification are being increasingly used for the etiological diagnosis of purulent infections, including SBP, septic arthritis, or CAPD-related peritonitis (4,7,8,12,18,19). These assays have been shown to improve the diagnostic efficiency of culture methods due to their higher sensitivities, especially with samples obtained from patients who have been exposed to antibiotics prior to specimen sampling.…”

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confidence: 99%

Performance of the LightCycler SeptiFast Test M ^grade in Detecting Microbial Pathogens in Purulent Fluids

et al. 2011

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The performance of the LightCycler SeptiFast (SF) assay was compared to that of culture methods in the detection of microorganisms in 43 purulent fluids from patients with pyogenic infections. The SF assay was more sensitive than the culture methods (86% versus 61%, respectively), irrespective of whether the infections were mono-or polymicrobial.The LightCycler SeptiFast (SF) test is a commercially available multiplex real-time PCR assay able to detect a wide range of bacterial and fungal organisms commonly involved in systemic infections (10). The SF assay has been shown to be a valuable ancillary method for the etiological diagnosis of bacteremia, sepsis, endocarditis, and more recently, periprosthetic joint infections, particularly in patients who have received antibiotics prior to diagnostic testing (1-3, 5, 6, 9, 10, 13-17). Molecular methods can allow the rapid detection of the microorganisms involved in severe pyogenic infections, thus leading to a more efficient and targeted early therapeutic intervention, which could translate into major clinical benefits for patients. To the best of our knowledge, there is no published experience on the performance characteristics of the SF assay for the detection of microorganisms in purulent fluids.A total of 43 purulent fluids obtained from 41 patients (29 male and 12 female; mean age, 64.1 years) over a period of 5 months (July to November 2010) were included in this study, as follows: 11 biliary fluid samples from patients with acute cholangitis, 7 pleural fluid samples from patients with empyema secondary to mesothelioma, lung cancer, or community-acquired and nosocomial pneumonia, 7 peritoneal fluid samples from patients with secondary peritonitis, 7 dialysis effluents from 6 patients with continuous ambulatory peritoneal dialysis (CAPD)-related peritonitis, 5 purulent liquid samples from 4 patients with intra-abdominal abscesses secondary to abdominal surgery, 4 purulent (Ͼ250 polymorphonuclear leukocytes/l) ascites fluid samples from cirrhotic patients with spontaneous bacterial peritonitis (SBP), and 2 intra-articular fluid samples from patients with acute septic arthritis. A total of 31 of the 41 patients had been treated with broad-spectrum antibiotics prior to sampling, as follows. All patients (n ϭ 11) undergoing biliary surgery, 3 patients subjected to thoracocentesis for empyema, and 3 patients undergoing laparotomy for secondary peritonitis received perioperative antimicrobial prophylaxis with amoxicillin-clavulanic. All patients with intra-abdominal abscesses and 4 patients with secondary peritonitis were empirically treated with ciprofloxacin plus metronidazole for a median of 2 days (range, 1 to 4 days) prior to specimen sampling. Four patients with secondary empyema had been treated with imipenem, imipenem plus metronidazole, or imipenem plus vancomycin for a median of 1 day prior to specimen sampling (range, 1 to 3 days). One patient with CAPD, one patient with SBP, and one patient with septic arthritis were treated with vancomycin or ceftriaxone ...

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Ureaplasma urealyticum Continuous Ambulatory Peritoneal Dialysis-Associated Peritonitis Diagnosed by 16S rRNA Gene PCR

Cited by 14 publications

References 12 publications

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

Optimization of Periprosthetic Culture for Diagnosis of Propionibacterium acnes Prosthetic Joint Infection

Performance of the LightCycler SeptiFast Test M ^grade in Detecting Microbial Pathogens in Purulent Fluids

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Ureaplasma urealyticum Continuous Ambulatory Peritoneal Dialysis-Associated Peritonitis Diagnosed by 16S rRNA Gene PCR

Cited by 14 publications

References 12 publications

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

Optimization of Periprosthetic Culture for Diagnosis of Propionibacterium acnes Prosthetic Joint Infection

Performance of the LightCycler SeptiFast Test M grade in Detecting Microbial Pathogens in Purulent Fluids

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Performance of the LightCycler SeptiFast Test M ^grade in Detecting Microbial Pathogens in Purulent Fluids