<i>Trypanosoma</i> sp. diversity in Amazonian bats (Chiroptera; Mammalia) from Acre State, Brazil

Santos, Francisco Charles Bezerra dos; Lisboa, Cristiane Varella; Xavier, Samanta Cristina das Chagas; Dario, Maria Augusta; Verde, Rair de Sousa; Calouro, Armando Muniz; Roque, André Luiz Rodrigues; Jansen, Ana Maria

doi:10.1017/s0031182017001834

Cited by 31 publications

(22 citation statements)

References 29 publications

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“…To date, most of these studies have concentrated on the T. c. cruzi and T. rangeli species, as high morphological, biological, biochemical, and molecular variability has been observed in these species [ 30 , 31 ]. Studies regarding differences among T. dionisii isolates are scarce, and most recent publications have focused solely on the molecular identification of this parasite in both hosts and vectors from Brazil and some other countries [ 7 , 8 , 10 , 11 , 12 , 18 ]. The three isolates of T. dionisii investigated (M1011, M1014, and M1015) were obtained in a study in different municipalities of Rio de Janeiro, where 22 samples of trypanosomatids were isolated by hemoculture of 84 bats [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Brazil, T. dionisii is found in almost all biomes, including the Amazon, Atlantic Forest, Cerrado, Pantanal, and transition areas of the Pantanal and Cerrado. The protozoan infects different bat families, including Phyllostomidae, Molossidae, Noctilionidae, and Vespertilionidae in various regions from northern to southern Brazil [ 4 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

et al. 2020

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This study describes the morphological, biochemical, and molecular differences among Trypanosoma dionisii isolates from hemocultures of hematophagous (Desmodus rotundus; n = 2) and insectivorous (Lonchorhina aurita; n = 1) bats from the Atlantic Rainforest of Rio de Janeiro, Brazil. Fusiform epimastigotes from the hematophagous isolates were elongated, whereas those of the insectivorous isolate were stumpy, reflected in statistically evident differences in the cell body and flagellum lengths. In the hemocultures, a higher percentage of trypomastigote forms (60%) was observed in the hematophagous bat isolates than that in the isolate from the insectivorous bat (4%), which demonstrated globular morphology. Three molecular DNA regions were analyzed: V7V8 (18S rDNA), glycosomal glyceraldehyde 3-phosphate dehydrogenase gene, and mitochondrial cytochrome b gene. The samples were also subjected to multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis. All isolates were identified as T. dionisii by phylogenetic analysis. These sequences were clustered into two separate subgroups with high bootstrap values according to the feeding habits of the bats from which the parasites were isolated. However, other T. dionisii samples from bats with different feeding habits were found in the same branch. These results support the separation of the three isolates into two subgroups, demonstrating that different subpopulations of T. dionisii circulate among bats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

et al. 2020

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“…[44–46]. The infection rate among bats roosting in palm trees appears to be higher in the Amazon compared to the Atlantic Forest [14,41,47].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of trypanosomatids, including Crithidia mellificae, in bats from the Atlantic Forest of Rio de Janeiro, Brazil

Rangel¹,

Lisboa²,

Novaes³

et al. 2019

PLoS Negl Trop Dis

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We studied infection by Trypanosomatidae in bats captured in two areas with different degradation levels in the Atlantic Forest of Rio de Janeiro state: Reserva Ecológica de Guapiaçu (REGUA) and Estação Fiocruz Mata Atlântica (EFMA). Furthermore, we evaluated whether the diversity of trypanosomatids changes according to bat diversity and the different levels of preservation in the region. The results showed no influence of the level of preservation on bat species richness (15 and 14 species, respectively), with similar chiropterofauna and higher abundance of two common fruit-eating bat species in the tropics: Carollia perspicillata and Artibeus lituratus . Of the 181 bat specimens analyzed by LIT/Schneider hemoculture, we detected 24 infected individuals (13%), including one positive Sturnira lilium individual that was also positive by fresh blood examination. Molecular characterization using nested PCR targeting the 18 SSU rRNA-encoding gene fragment showed similar trypanosomatid infection rates in bats from the two areas: 15% in REGUA and 11% in EFMA ( p = 0.46). Trypanosoma dionisii was the most frequently detected parasite (54%), followed by T . cruzi DTUs TcI and TcIV and Trypanosoma sp., in Neotropical phyllostomid bats (RNMO63 and RNMO56); mixed infections by T . dionisii / T . cruzi TcIII and T . dionisii / T . cruzi TcI were also observed. The T . cruzi DTUs TcI and TcIV are the genotypes currently involved in cases of acute Chagas disease in Brazil, and T . dionisii was recently found in the heart tissue of an infected child. Surprisingly, we also describe for the first time Crithidia mellificae , a putative monoxenous parasite from insects, infecting a vertebrate host in the Americas. Bats from the Atlantic Forest of Rio de Janeiro state harbor a great diversity of trypanosomatids, maintaining trypanosomatid diversity in this sylvatic environment.

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“…The TSSApep-II/V/VI seropositives identified here by Chagas Sero K-SeT were from the neighbouring state of Piauí. Similarly, a single bat specimen was positive by Chagas Sero K-Set; T. cruzi lineages have been reported from Brazilian bats, by genotyping [38,39].…”

Section: Discussionmentioning

confidence: 89%

A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

et al. 2020

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Trypanosoma cruzi, the protozoan agent of Chagas disease in the Americas, is comprised of six genetic lineages (TcI-TcVI) and a possible seventh (TcBat, related to TcI). Identification of T. cruzi lineages infecting reservoir mammalian species is fundamental to resolving transmission cycles. However, this is hindered by the limited sensitivity and technical complexity of parasite isolation and genotyping. An alternative approach is serology using T. cruzi lineage-specific epitopes, such as those of the trypomastigote small surface antigen (TSSA). For surveillance of T. cruzi lineage infections in mammal species from diverse Brazilian regions, we apply a novel rapid diagnostic test (RDT, Chagas Sero K-SeT), which incorporates the TSSA peptide epitope specific to TcII/V/VI (TSSApep-II/V/VI) and Protein G detection of antibodies. Chagas Sero K-SeT RDT results with sera from experimentally infected mice, from tamarin primates (Leontopithecus spp.) and from canines (Canis familiaris) were concordant with corresponding TSSApep-II/V/VI ELISAs. The Chagas Sero K-Set detected TcII/V/VI infections in Leontopithecus spp. from the Atlantic forest (n = 46), in C. familiaris (n = 16) and Thrichomys laurentius (n = 2) from Caatinga biome and Chiroptera (n = 1) from Acre, Amazonia. The Chagas Sero K-SeT RDT is directly applicable to TcII/V/ VI-specific serological surveillance of T. cruzi infection in several different mammalian Orders. It can replace ELISAs and provides efficient, point-of-sampling, low-cost detection of TcII/V/VI infections, with at least equivalent sensitivity, although some mammals may be difficult to trap, and, not unexpectedly, Chagas Sero K-SeT could not recognise feline IgG. Knowledge of sylvatic hosts of T. cruzi can be expanded, new reservoir species discovered, and the ecology of transmission cycles clarified, particularly with adaptation to further mammalian Orders.

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Trypanosoma sp. diversity in Amazonian bats (Chiroptera; Mammalia) from Acre State, Brazil

Cited by 31 publications

References 29 publications

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

Isolation and characterization of trypanosomatids, including Crithidia mellificae, in bats from the Atlantic Forest of Rio de Janeiro, Brazil

A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

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