<i>Trifolium pratense</i>extract increases testosterone and improves sperm characteristics and antioxidant status in diabetic rats

Khazaei, Mozafar; Gravandi, Elham; Ghanbari, Elham; Niromand, Elham

doi:10.1080/10520295.2022.2039766

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…16 A large number of studies have shown that longterm hyperglycemia increases the production of ROS in testicular tissue of patients with diabetes and plays a key role in male testicular dysfunction. 17 At the same time, it has been confirmed in previous animal models that excessive production of ROS in testis can induce autophagy. 18 Autophagy is a degradation pathway of intracellular lysosomes, which plays an important role in maintaining the balance of intracellular environment.…”

mentioning

confidence: 87%

“…Some studies have shown that spermatozoa are vulnerable to oxidative damage, excessive reactive oxygen species cause pathological changes in sperm, and the imbalance between the production of reactive oxygen species and antioxidant capacity of sperm will lead to male infertility 16 . A large number of studies have shown that long‐term hyperglycemia increases the production of ROS in testicular tissue of patients with diabetes and plays a key role in male testicular dysfunction 17 . At the same time, it has been confirmed in previous animal models that excessive production of ROS in testis can induce autophagy 18 …”

Section: Introductionmentioning

confidence: 99%

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The role of stimulator of interferon genes‐mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin‐induced testicular injury

Zhao

Xie

et al. 2022

Environmental Toxicology

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Cyfluthrin is widely used in the field of sanitary pest control by its wide insecticidal spectrum, high efficiency and low toxicity, low residue, and good biodegradability. But, as a double‐edged sword, a large amount of cyfluthrin remains are still in the environment. The residual cyfluthrin is absorbed into the food chain through vegetation and then poses a risk to soil organisms and human health. Several studies have suggested that cyfluthrin is one of the main factors causing testicular damage, but the mechanism remains unclear. In this study, we established in vivo and in vitro models of testicular injury in rats and GC‐2 cells exposed to cyfluthrin to explore whether stimulator of interferon genes (STING) gene mediates the regulation of AMPK/mTOR/p70S6K autophagy pathway, which lays a foundation for further study of the mechanism of testicular injury induced by cyfluthrin. The results showed that the activity of super oxide dismutase in testis decreased and the activity of malonic dialdehyde increased with the increase of concentration in vivo and in vitro. At the same time, the levels of mitochondrial damage and inflammation in the testis also increased, which further activated autophagy. In this process, the increased level of inflammation is related to the increased expression of STING gene, and AMPK/mTOR/p70S6K autophagy pathway is also involved. To sum up, cyfluthrin has certain reproductive toxicity, and long‐term exposure can induce testicular cell damage. STING gene can participate in cyfluthrin‐induced testicular injury through AMPK/mTOR/P70S6K autophagy pathway.

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mentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

The role of stimulator of interferon genes‐mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin‐induced testicular injury

Zhao

Xie

et al. 2022

Environmental Toxicology

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“…[15,18,[29][30][31] At the end of the treatment period, the animals were weighed and then killed. [32] The testes and epididymides were collected by laparotomy for sperm analysis. One testis fixed for histopathology and TUNEL evaluation and the other one fresh-frozen for oxidant/antioxidant assessment.…”

Section: Cctl and Vit-c Treatment Protocolsmentioning

confidence: 99%

Utility of vitamin C in ameliorating citalopram‐induced testicular toxicity via modulating nitro‐oxidative stress and apoptosis in mice

Moradi,

Hashemian,

Fathi

et al. 2023

J Biochem & Molecular Tox

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There is a growing concern that antidepressant drugs impair sexual function and adversely impact spermatogenesis and male fertility. Vitamin C is a natural antioxidant that plays a vital role in the male reproductive system. The present study investigated the ameliorating potential of vitamin C against citalopram (CTL)‐evoked testicular toxicity and spermatogenesis impairment in mice. Mice were randomly divided into six groups: control, CTL, vitamin C 100, vitamin C 200, CTL plus vitamin C 100, and CTL plus vitamin C 200. Adult male mice were intraperitoneally (ip) injected with 10 mg/kg of CTL for 35 days with or without vitamin C. At the end of the study, body and testes weight, sperm parameters, histopathology of testes, testosterone level, testicular levels of malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC), and apoptosis (TUNEL assay) were evaluated. Our findings revealed that vitamin C restored spermatogenesis by improving sperm count, motility, viability, morphology, and chromatin integrity. Testosterone levels and testes histopathology were significantly improved in the vitamin C‐administrated groups. Furthermore, vitamin C administration markedly alleviated CTL‐induced nitro‐oxidative damage, enhancing TAC levels, and reducing NO and MDA levels. Whilst CTL therapy induced a significant increase in the number of TUNEL‐positive cells compared to the control, the administration of vitamin C significantly prevented the apoptotic effects of CTL. Together, vitamin C therapy protects against CTL‐induced testicular damage via mitigating nitro‐oxidative stress and apoptosis, which provides evidence for vitamin C as a beneficial therapy against antidepressant drug‐associated reproductive toxicity and male sub/infertility.

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“…Therefore, there is an urgent need to implement safe compounds with plausible protective effects against oxidative and inflammatory changes induced by diabetes. In this regard, several studies have demonstrated the benefits of various medicinal plants and their derived bioactive phytochemicals in the management of diabetes-induced male infertility [59][60][61][62]. Lepidium sativum L., also known as Garden cress/Hab El Rashad, is a comparatively fast-growing annual herb that belongs to the family Brassicaceae [63].…”

Section: Introductionmentioning

confidence: 99%

Garden Cress Seed Oil Abrogates Testicular Oxidative Injury and NF-kB-Mediated Inflammation in Diabetic Mice

Abu-Khudir,

Badr,

Abd El-Moaty

et al. 2023

IJMS

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Diabetes mellitus is a metabolic disorder associated with various complications encompassing male reproductive dysfunction. The present study aimed to investigate the therapeutic potential of biologically active Lepidium sativum seed oil (LSO) against the testicular dysfunction associated with streptozotocin (STZ)-induced diabetes. Male adults (n = 24) were divided into four groups: control, LSO-administered, diabetic (D), and LSO-treated diabetic (D+LSO) groups. LSO was extracted from L. sativum seeds, and its chemical composition was determined using GC-MS. Serum testosterone levels, testicular enzymatic antioxidants (catalase (CAT) and superoxide dismutase (SOD)), an oxidative stress (OS) biomarker, malondialdehyde (MDA), pro-inflammatory markers (NF-kB, IL-1, IL-6, and TNF-α), and the expression level of NF-kB were assessed. In addition, histopathological changes were evaluated in testicular tissues. The results obtained showed that the chemical composition of LSO indicated its enrichment mainly with γ-tocopherol (62.1%), followed by 2-methylhexacosane (8.12%), butylated hydroxytoluene (8.04%), 10-Methylnonadecane (4.81%), and δ-tocopherol (3.91%). Moreover, LSO administration in the D+LSO mice significantly increased testosterone levels and ameliorated the observed testicular oxidative damage, inflammatory response, and reduced NF-kB expression compared to the diabetic mice. Biochemical and molecular analyses confirmed the histological results. In conclusion, LSO may prevent the progression of diabetes-induced impairment in the testes through inhibition of the OS- and NF-kB-mediated inflammatory response.

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Trifolium pratenseextract increases testosterone and improves sperm characteristics and antioxidant status in diabetic rats

Cited by 4 publications

References 22 publications

The role of stimulator of interferon genes‐mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin‐induced testicular injury

The role of stimulator of interferon genes‐mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin‐induced testicular injury

Utility of vitamin C in ameliorating citalopram‐induced testicular toxicity via modulating nitro‐oxidative stress and apoptosis in mice

Garden Cress Seed Oil Abrogates Testicular Oxidative Injury and NF-kB-Mediated Inflammation in Diabetic Mice

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