<i>Trichoderma asperelloides</i> antagonism to nine <i>Sclerotinia sclerotiorum</i> strains and biological control of white mold disease in soybean plants

Sumida, Ciro Hideki; Daniel, Juliana F. S.; Araujod, Ana Paula C. S.; Peitl, Douglas Casaroto; Abreu, Lucas M.; Dekker, Robert F. H.; Canteri, Marcelo Giovanetti

doi:10.1080/09583157.2018.1430743

Cited by 43 publications

(23 citation statements)

References 39 publications

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“…Trichoderma asperelloides has been used as a biocontrol agent of Sclerotinia sclerotiorum [17]. However, the ability of the VOCs emitted by T. asperelloides PSU-P1 to contribute to antifungal activity and to promote plant growth has not yet been clarified.…”

Section: Introductionmentioning

confidence: 99%

Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana

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Suwannarach

Lumyong

et al. 2020

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Fungal volatile organic compounds (VOCs) emitted by Trichoderma species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by Trichoderma asperelloides PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to promote plant growth in Arabidopsis thaliana. The strain’s VOCs had remarkable antifungal activity against fungal pathogens, with an inhibition range of 15.92–84.95% in a volatile antifungal bioassay. The VOCs of T. asperelloides PSU-P1 promoted the plant growth of A. thaliana, thereby increasing the fresh weight, root length, and chlorophyll content in the VOC-treated A. thaliana relative to those of the control. High expression levels of the chitinase (CHI) and β-1,3-glucanase (GLU) genes were found in the VOC-treated A. thaliana by quantitative reverse transcription polymerase chain reaction (RT-PCR). The VOC-treated A. thaliana had higher defense-related enzyme (peroxidase (POD)) and cell wall-degrading enzyme (chitinase and β-1,3-glucanase) activity than in the control. The headspace VOCs produced by PSU-P1, trapped with solid phase microextraction, and tentatively identified by gas chromatography–mass spectrometry, included 2-methyl-1-butanol, 2-pentylfuran, acetic acid, and 6-pentyl-2H-pyran-2-one (6-PP). The results suggest that T. asperelloides PSU-P1 emits VOCs responsible for antifungal activity, for promoting plant growth, and for inducing defense responses in A. thaliana.

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Section: Introductionmentioning

confidence: 99%

Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana

Phoka

Suwannarach

Lumyong

et al. 2020

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“…Different results were present for Stefanello et al (29), that using Trichoderma harzianum in the control of root rot of cassava, observed increased productivity for the treatments with the use of bioagent. Sumida et al (30) and Haddad et al (13) also observed an antagonistic reaction of Trichoderma to isolates of Sclerotinia sclerotiorum, that causes white mold. Méndez-Bravo et al (21) demonstrated that volatile compounds produced by bacterial isolates from rhizosphere showed biocontrol of soil borne oomycetes such as P. cinnamomi and the antagonism can be related to metabolites produced by bioagents.…”

Section: Chemical and Biological Treatment Of Avocado Seedlings Inocumentioning

confidence: 94%

Control of root rot (Phytophthora cinnamomi) in avocado (Persea Americana) with bioagents

et al. 2020

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Despite the favorable edaphoclimatic conditions for avocado production in Brazil, diseases such as root rot caused by the pathogen Phytophthora cinnamomi compromise the crop. With the aim of managing root rot in avocado, the present study aimed to evaluate chemical and biological control with isolates of Trichoderma spp. and Pseudomonas fluorescens. Thus, three assays were conducted to assess: (i) mycelial inhibition of P. cinnamomi by isolates of Trichoderma spp. and P. fluorescens from different crop systems; (ii) effect of autoclaved and non-autoclaved metabolites of P. fluorescens, and (iii) chemical or biological treatment of avocado seedlings on the control of root rot under field conditions. The isolates of Trichoderma spp. from maize cultivation soil and the commercial products formulated with Trichoderma presented greater antagonism (p <0.05) to the pathogen P. cinnamomi in the in vitro tests. Similarly, non-autoclaved metabolites of P. fluorescens presented antagonistic potential to control P. cinnamomi. Under field conditions, the fungicide metalaxyl and the bioagents showed effectiveness in controlling P. cinnamomi, as well as greater root length and mass. Results demonstrated potential for the biological control of avocado root rot with Trichoderma spp. and P. fluorescens.

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“…These structures hamper disease control (Mendgen and Hahn 2002). Currently, control of white mould disease is based on integrated management practices (Reis and Lopes 2007;Williams 2007;Wyenandt 2008;Boat et al 2018;Sumida et al 2018). After the establishment of the disease, chemical compounds are usually used; however, their usage contributes to increasing the market price of the final product, and they promote environmental damage (Gorgen 2009;Harveson et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Validation of an in vitro system to trigger changes in the gene expression of effectors of Sclerotinia sclerotiorum

et al. 2021

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Aims: Sclerotinia sclerotiorum, the causal agent of white mold, can infect several host species, including economically important crops. In this study, we propose and validate a new in vitro system able to mimic the conditions of interaction with the host and promote the induction of S. sclerotiorum effectors. Methods and results: For culture media production, we selected three plant species, common bean (Phaseolus vulgaris L, cv. Requinte.), maize (Zea mays, cv. BRS1030) and beggarticks (Bidens pilosa). To validate this system as an in vitro inducer of effectors, the qRT-PCR technique was used to investigate the expression profile of some S. sclerotiorum effector genes in each growth medium at different times after inoculation. Conclusion:The results obtained in this study provide a validation of a new method to study S. sclerotiorum during mimetic interaction with different hosts. Although leaf extract does not fully represent the plant environment, the presence of plant components in the culture medium seems to induce effector genes, mimicking in planta conditions. The use of MEVM is simpler than in planta growth, bypasses problems such as the amount of mycelium produced, as well as contamination of host cells during transcriptomic and proteomic analyses. Significance and Impact of the Study: We have devised MEVM media as a model mimicking the interaction of S. sclerotiorum and its hosts and used it to evaluate in vitro expression of effectors normally expressed only in planta.

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Trichoderma asperelloides antagonism to nine Sclerotinia sclerotiorum strains and biological control of white mold disease in soybean plants

Cited by 43 publications

References 39 publications

Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana

Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana

Control of root rot (Phytophthora cinnamomi) in avocado (Persea Americana) with bioagents

Validation of an in vitro system to trigger changes in the gene expression of effectors of Sclerotinia sclerotiorum

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