TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum

Jiang, Cong; Zhang, Chengkang; Wu, Chunlan; Sun, Panpan; Hou, Rui; Liu, Huiquan; Wang, Chenfang; Xu, Jin‐Rong

doi:10.1111/1462-2920.13279

Cited by 95 publications

(82 citation statements)

References 60 publications

(101 reference statements)

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“…The AreA transcription factor from F. graminearum was shown to mediate the regulation of DON synthesis by cAMP signaling (Hou et al, 2015). Recently, it was reported that cAMP signaling pathway is involved in the regulation of DON biosynthesis by two pathway-specific transcription factors TRI (trichothecene biosynthesis) 6 and TRI10 (Jiang et al, 2016). Therefore, these findings suggest significant roles for cAMP-PKA signaling pathway in multiple physiological processes of different microorganisms, especially during the steps of surface recognition and penetration which are critical in the infection cycle of many plant pathogenic fungi.…”

Section: Introductionmentioning

confidence: 99%

The cAMP-PKA Signaling Pathway Regulates Pathogenicity, Hyphal Growth, Appressorial Formation, Conidiation, and Stress Tolerance in Colletotrichum higginsianum

et al. 2017

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Colletotrichum higginsianum is an economically important pathogen that causes anthracnose disease in a wide range of cruciferous crops. Understanding the mechanisms of the cruciferous plant–C. higginsianum interactions will be important in facilitating efficient control of anthracnose diseases. The cAMP-PKA signaling pathway plays important roles in diverse physiological processes of multiple pathogens. C. higginsianum contains two genes, ChPKA1 and ChPKA2, that encode the catalytic subunits of cyclic AMP (cAMP)-dependent protein kinase A (PKA). To analyze the role of cAMP signaling pathway in pathogenicity and development in C. higginsianum, we characterized ChPKA1 and ChPKA2 genes, and adenylate cyclase (ChAC) gene. The ChPKA1 and ChAC deletion mutants were unable to cause disease and significantly reduced in hyphal growth, tolerance to cell wall inhibitors, conidiation, and appressorial formation with abnormal germ tubes, but they had an increased tolerance to elevated temperatures and exogenous H2O2. In contrast, the ChPKA2 mutant had no detectable alteration of phenotypes, suggesting that ChPKA1 contributes mainly to PKA activities in C. higginsianum. Moreover, we failed to generate ΔChPKA1ChPKA2 double mutant, indicating that deletion of both PKA catalytic subunits is lethal in C. higginsianum and the two catalytic subunits possibly have overlapping functions. These results indicated that ChPKA1 is the major PKA catalytic subunit in cAMP-PKA signaling pathway and plays significant roles in hyphal growth, pathogenicity, appressorial formation, conidiation, and stress tolerance in C. higginsianum.

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Section: Introductionmentioning

confidence: 99%

The cAMP-PKA Signaling Pathway Regulates Pathogenicity, Hyphal Growth, Appressorial Formation, Conidiation, and Stress Tolerance in Colletotrichum higginsianum

et al. 2017

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“…In many plant-pathogenic fungi, cAMP signaling is also involved in toxin production and virulence (D'Souza and Heitman, 2001; Lee et al, 2003; Shimizu et al, 2003; Choi and Xu, 2010; Hu et al, 2014). For example, in F. graminearum , cAMP signaling has been shown to be important for the production of DON, which is one of the best characterized virulence factors (Hu et al, 2014; Jiang et al, 2016). In A .…”

Section: Introductionmentioning

confidence: 99%

Adenylate Cyclase AcyA Regulates Development, Aflatoxin Biosynthesis and Fungal Virulence in Aspergillus flavus

Yang

Qin

Liu

et al. 2016

Front. Cell. Infect. Microbiol.

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Aspergillus flavus is one of the most important opportunistic pathogens of crops and animals. The carcinogenic mycotoxin, aflatoxins produced by this pathogen cause a health problem to human and animals. Since cyclic AMP signaling controls a range of physiological processes, like fungal development and infection when responding to extracellular stimuli in fungal pathogens, in this study, we investigated the function of adenylate cyclase, a core component of cAMP signaling, in aflatoxins biosynthesis and virulence on plant seeds in A. flavus. A gene replacement strategy was used to generate the deletion mutant of acyA that encodes the adenylate cyclase. Severe defects in fungal growth, sporulation and sclerotia formation were observed in the acyA deletion mutant. The defect in radical growth could be partially rescued by exogenous cAMP analog. The acyA mutant was also significantly reduced in aflatoxins production and virulence. Similar to the former studies in other fungi, The acyA mutant showed enhancing tolerance to oxidative stress, but more sensitive to heat stress. Overall, the pleiotropic defects of the acyA deletion mutant indicates that the cAMP-PKA pathway is involved in fungal development, aflatoxins biosynthesis and plant seed invasion in A. flavus.

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“…The Fermentas First cDNA synthesis kit (Hanover, MD, USA) was used for cDNA synthesis. The TUB2 beta-tubulin gene was used as the internal control 34 and the relative expression of each gene was calculated with the 2 −△△Ct method. Data from three biological replicates were used to calculate the mean and standard deviation of the expression levels 35 .…”

Section: Methodsmentioning

confidence: 99%

RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

Cao

Hao

et al. 2017

Sci Rep

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Ascospores are the primary inoculum in the wheat scab fungus Fusarium graminearum that was recently shown to have sexual stage-specific A-to-I RNA editing. One of the genes with premature-stop-codons requiring A-to-I editing to encode full-length functional proteins is AMD1 that encodes a protein with a major facilitator superfamily (MFS) domain. Here, we characterized the functions of AMD1 and its UAG to UGG editing event. The amd1 deletion mutant was normal in growth and conidiation but defective in ascospore discharge due to the premature breakdown of its ascus wall in older perithecia, which is consistent with the specific expression of AMD1 at later stages of sexual development. Expression of the wild-type or edited allele of AMD1 but not un-editable allele rescued the defects of amd1 in ascospore discharge. Furthermore, Amd1-GFP localized to the ascus membrane and Amd1 orthologs are only present in ascocarp-forming fungi that physically discharge ascospores. Interestingly, deletion of AMD1 results in the up-regulation of a number of genes related to transporter activity and membrane functions. Overall, these results indicated that Amd1 may play a critical role in maintaining ascus wall integrity during ascus maturation, and A-to-I editing of its transcripts is important for ascospore discharge in F. graminearum.

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TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum

Cited by 95 publications

References 60 publications

The cAMP-PKA Signaling Pathway Regulates Pathogenicity, Hyphal Growth, Appressorial Formation, Conidiation, and Stress Tolerance in Colletotrichum higginsianum

The cAMP-PKA Signaling Pathway Regulates Pathogenicity, Hyphal Growth, Appressorial Formation, Conidiation, and Stress Tolerance in Colletotrichum higginsianum

Adenylate Cyclase AcyA Regulates Development, Aflatoxin Biosynthesis and Fungal Virulence in Aspergillus flavus

RNA editing of the AMD1 gene is important for ascus maturation and ascospore discharge in Fusarium graminearum

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