<i>trans</i>-10,<i>cis</i>-12-Conjugated Linoleic Acid Affects Expression of Lipogenic Genes in Mammary Glands of Lactating Dairy Goats

Shi, Huaiping; Zhang, Tianying; Liu, Cong; Wang, Jianjue; Huang, Jun; Li, Zhongyang

doi:10.1021/acs.jafc.7b02377

Cited by 21 publications

(26 citation statements)

References 35 publications

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“…To our knowledge, this was the first study that evaluated effect of CLA on gene expression in mammary gland and adipose tissue of lactating sows; therefore, comparisons were made with ruminants or growing pigs. As in our study, previous works in cows, ewes and goats have established the anti-lipogenic effect of the trans-10, cis-12-CLA and have shown that its causes a reduction in the expression of genes involved in many lipid synthesis enzymes including ACACAα, FASN, SCD1, LPL, AGPAT and DGAT (24,27,46) .…”

Section: Discussionsupporting

confidence: 77%

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Trans-10,cis-12 conjugated linoleic acid reduces milk fat content and lipogenic gene expression in the mammary gland of sows without altering litter performance

2019

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Trans-10, cis-12 conjugated linoleic acid (CLA) decreases milk fat synthesis in lactating sows and involves, at least in part, the down-regulation of lipogenic genes. The objective was to evaluate the effect of CLA on milk composition and lipogenic gene expression. Twenty multiparous sows were randomly assigned to one of the two treatments for 18 d (from day 7 to day 25 of lactation): (1) control (no CLA added) and (2) 1 % of CLA mixed into the ration. CLA treatment decreased milk fat and protein content by 20 % (P = 0·004) and 11 % (P = 0·0001), respectively. However, piglet weight did not differ between treatments (P = 0·60). Dietary CLA increased the concentration of SFA in milk fat by 16 % (P < 0·0001) and decreased MUFA by 17·6 % (P < 0·0001). In the mammary gland, CLA reduced gene expression of acetyl-CoA carboxylase-α by 37 % (P = 0·003), fatty acid synthase by 64 % (P = 0·002), stearoyl-CoA desaturase 1 by 52 % (P = 0·003), lipoprotein lipase by 26 % (P = 0·03), acyl glycerol phosphate acyltransferase 6 by 15 % (P = 0·02) and diacylglycerol acyltransferase 1 by 27 % (P = 0·02), whereas the expression of fatty acid binding protein 3 was not altered by CLA treatment (P = 0·09). Mammary expression of casein-β and α-lactalbumin was reduced by CLA by 68 % (P = 0·0004) and 62 % (P = 0·005), respectively. Additionally, CLA had no effect on the expression of lipogenic genes evaluated in adipose tissue. In summary, CLA reduced milk fat content without negatively affecting litter performance and it affected mammary expression of genes involved in all lipogenic pathways studied.

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Section: Discussionsupporting

confidence: 77%

“…coordinated down-regulation in the transcript abundance of genes involved in milk fat synthesis in the mammary gland of lactating cows (24) and bovine mammary primary cells and cell lines (25) and also in ewes (26) and goats (27) , caused by supplementation with trans-10, cis-12-CLA.…”

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confidence: 99%

Trans-10,cis-12 conjugated linoleic acid reduces milk fat content and lipogenic gene expression in the mammary gland of sows without altering litter performance

2019

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“…We further reveal that the regulatory role of FABP5 in Met‐ and E2‐induced SREBP‐1c gene expression depends on the transport of fatty acids by FABP5 from the external environment into cells. Previous reports have shown that fatty acids are inducers of gene expression of FABPs and SREBP‐1c (Duplus & Forest, ; N. Li et al, ; Shi et al, ). Fatty acids are the main substrates of lipogenesis (Bauman et al, ).…”

Section: Discussionmentioning

confidence: 98%

“…Previous reports have shown that fatty acids are inducers of gene expression of FABPs and SREBP-1c (Duplus & Forest, 2002; N. Shi et al, 2017). Fatty acids are the main substrates of lipogenesis (Bauman et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

FABP5 is a critical regulator of methionine‐ and estrogen‐induced SREBP‐1c gene expression in bovine mammary epithelial cells

Zhou

et al. 2018

Journal Cellular Physiology

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The intracellular fatty acid-binding proteins (FABPs) are a well-conserved family that function as lipid chaperones. Ongoing studies are focused on identification of the mechanistic complexity and vast biological diversity of different isoforms of FABPs. However, the molecular mechanism of FABP5 in the regulation of milk fat synthesis in the mammary gland of dairy cows is still largely unknown. Here, we report that FABP5 acts as a critical regulator of terol response element-binding protein-1c (SREBP-1c) gene expression induced by methionine (Met) and estrogen (E2) in bovine mammary epithelial cells (BMECs). We observed that the expression of FABP5 was markedly higher in dairy cow mammary tissue during the lactating period than the puberty period and the dry period. FABP5 is located in the cytoplasm, and Met and E2 significantly increase the protein levels of FABP5 in BMECs. Using gene function study approaches, we revealed that FABP5 positively regulates SREBP-1c gene expression and promotes milk fat synthesis. We confirmed that FABP5 is required for Met- and E2-induced SREBP-1c gene expression and milk fat synthesis. We further uncovered that fatty acids are needed for FABP5-mediated SREBP-1c gene expression. Thus, our study demonstrates that FABP5 is a critical regulator of Met- and E2-induced SREBP-1c gene expression leading to milk fat synthesis.

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“…In cows, the abomasal infusion of trans ‐10, cis ‐12 CLA causes a MFD with a concomitant decrease in milk fat yield (−48%) and lipogenic gene expression (FASN, ACACA, SCD, LPL, FABP, GPAT, AGPAT) . In goats, whereas addition of trans ‐10, cis ‐12 CLA at 0.2% DM had no effect on milk fat yield, a decrease of −19% was observed for an addition of 0.4% DM without impacting mammary lipogenic gene expression …”

Section: Effects Of Dietary Lipids On Lipid Metabolism In Dairy Ruminmentioning

confidence: 99%

Milk Fat Globule in Ruminant: Major and Minor Compounds, Nutritional Regulation and Differences Among Species

Bernard

Bonnet

Delavaud

et al. 2018

Euro J Lipid Sci & Tech

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Recent knowledge is presented on the composition of ruminant milk fat fractions and the nutritional strategies known to alter their amount. The development of lipidomic and proteomic analyses has allowed for the characterization of minor components, such as proteins, liposoluble vitamins, and phospholipids of the milk fat globule (MFG), in addition to the triacylglycerols (TAG), which are the major constituents of the MFG core. Few differences in these components among ruminant species exist, and they have been outlined mainly on the fatty acids (FA) profile of the TAG, whereas comparative data are still lacking on vitamins and proteins. The effects of dietary treatments enriched in n‐3 polyunsaturated FA (PUFA) on the composition of the milk fat fraction are explored. In particular, pasture and plant oilseeds increase milk n‐3 PUFA and cis‐9,trans‐11 CLA and decrease saturated FA, whereas data with new feed resources, such as algae, are still rare. The peculiarities of the response of the milk fat to diets that induce a milk fat depression in cows but in lesser extent in small ruminants are described. The potential effects of polar lipids, proteins, and liposoluble vitamins of the MFG on human health are reviewed, highlighting the nutraceutical properties of milk. Practical Applications: This review provides an overview of the different components of the milk fat fraction in ruminant species and on nutritional strategies to alter their amounts to improve the nutritional quality of milk. Furthermore, this review presents recent data on species peculiarities of the milk fat fraction composition and of its response to nutritional factors, which offers a promising model to identify news levers of regulation of this fraction and foster the identification of new feeding strategies to better control milk fat composition and feed efficiency. This review synthesizes data on the composition of the milk fat fraction in ruminant species, reports advances in nutritional strategies to alter their amounts and species‐specific responses to nutrition, as well as the potential effects on human health of the major and minor components of this fraction.

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trans-10,cis-12-Conjugated Linoleic Acid Affects Expression of Lipogenic Genes in Mammary Glands of Lactating Dairy Goats

Cited by 21 publications

References 35 publications

Trans-10,cis-12 conjugated linoleic acid reduces milk fat content and lipogenic gene expression in the mammary gland of sows without altering litter performance

Trans-10,cis-12 conjugated linoleic acid reduces milk fat content and lipogenic gene expression in the mammary gland of sows without altering litter performance

FABP5 is a critical regulator of methionine‐ and estrogen‐induced SREBP‐1c gene expression in bovine mammary epithelial cells

Milk Fat Globule in Ruminant: Major and Minor Compounds, Nutritional Regulation and Differences Among Species

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