<i>Tonoplast Sugar Transporters (SbTSTs)</i> putatively control sucrose accumulation in sweet sorghum stems

Bihmidine, Saadia; Julius, Benjamin T.; Dweikat, İsmail; Braun, David

doi:10.1080/15592324.2015.1117721

Cited by 59 publications

(54 citation statements)

References 36 publications

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“…Three gene families encoding sucrose transporters have been implicated in sugar accumulation in sorghum, including H + /sucrose antiporters located on tonoplast TSTs (Bihmidine et al ., ), H + /sucrose symporters SUTs (Bihmidine et al ., ; Leach et al ., ; Milne et al ., ), and the clade III of SWEET (Eom et al ., ; Mizuno et al ., ). We compared expression patterns of these gene families between the three genotypes (Figure S20).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and metabolome reveal distinct carbon allocation patterns during internode sugar accumulation in different sorghum genotypes

Wang

Feng

et al. 2018

Plant Biotechnology Journal

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Sweet sorghum accumulates large amounts of soluble sugar in its stem. However, a system-based understanding of this carbohydrate allocation process is lacking. Here, we compared the dynamic transcriptome and metabolome between the conversion line R9188 and its two parents, sweet sorghum RIO and grain sorghum BTx406 that have contrasting sugar-accumulating phenotypes. We identified two features of sucrose metabolism, stable concentrations of sugar phosphates in RIO and opposite trend of trehalose-6-phosphate (T6P) between RIO vs R9188/BTx406. Integration of transcriptome and metabolome revealed R9188 is partially active in starch metabolism together with medium sucrose level, whereas sweet sorghum had the highest sucrose concentration and remained highly active in sucrose, starch, and cell wall metabolism post-anthesis. Similar expression pattern of genes involved in sucrose degradation decreased the pool of sugar phosphates for precursors of starch and cell wall synthesis in R9188 and BTx406. Differential T6P signal between RIO vs R9188/BTx406 is associated with introgression of T6P regulators from BTx406 into R9188, including C-group bZIP and trehalose 6-phosphate phosphatase (TPP). The inverted T6P signalling in R9188 appears to down-regulate sucrose and starch metabolism partly through transcriptome reprogramming, whereas introgressed metabolic genes could be related to reduced cell wall metabolism. Our results show that coordinated primary metabolic pathways lead to high sucrose demand and accumulation in sweet sorghum, providing us with targets for genetic improvements of carbohydrate allocation in bioenergy crops.

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Section: Resultsmentioning

confidence: 99%

Transcriptome and metabolome reveal distinct carbon allocation patterns during internode sugar accumulation in different sorghum genotypes

Wang

Feng

et al. 2018

Plant Biotechnology Journal

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“…Control of hydrostatic pressure is facilitated by the sequestration of sucrose into the vacuole by tonoplast localized SUTs or into the apoplasm by plasma membrane localized SUTs (Slewinski, 2011). Members of the SUT and TMT families have been shown to function on the tonoplast to facilitate sucrose accumulation in the vacuole (Reinders et al, 2008; Wingenter et al, 2010; Bihmidine et al, 2016). In mature stem tissue plasma membrane localized SWEETs, SUTs, and possibly some NIPs may have a role in transporting sugar into the apoplasm (Milne et al, 2013; Chen, 2014).…”

Section: Discussionmentioning

confidence: 99%

Roles of Aquaporins in Setaria viridis Stem Development and Sugar Storage

et al. 2016

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Setaria viridis is a C4 grass used as a model for bioenergy feedstocks. The elongating internodes in developing S. viridis stems grow from an intercalary meristem at the base, and progress acropetally toward fully expanded cells that store sugar. During stem development and maturation, water flow is a driver of cell expansion and sugar delivery. As aquaporin proteins are implicated in regulating water flow, we analyzed elongating and mature internode transcriptomes to identify putative aquaporin encoding genes that had particularly high transcript levels during the distinct stages of internode cell expansion and maturation. We observed that SvPIP2;1 was highly expressed in internode regions undergoing cell expansion, and SvNIP2;2 was highly expressed in mature sugar accumulating regions. Gene co-expression analysis revealed SvNIP2;2 expression was highly correlated with the expression of five putative sugar transporters expressed in the S. viridis internode. To explore the function of the proteins encoded by SvPIP2;1 and SvNIP2;2, we expressed them in Xenopus laevis oocytes and tested their permeability to water. SvPIP2;1 and SvNIP2;2 functioned as water channels in X. laevis oocytes and their permeability was gated by pH. Our results indicate that SvPIP2;1 may function as a water channel in developing stems undergoing cell expansion and SvNIP2;2 is a candidate for retrieving water and possibly a yet to be determined solute from mature internodes. Future research will investigate whether changing the function of these proteins influences stem growth and sugar yield in S. viridis.

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“…Multiple classes of transporters involved in Suc flux across cell membranes have been identified, including Suc transporters (SUTs; Aoki et al, 2003;Lalonde et al, 2004;Sauer, 2007;Kühn and Grof, 2010;Ainsworth and Bush, 2011;Ayre, 2011;Baker et al, 2012;Reinders et al, 2012;Eom et al, 2015;Jung et al, 2015;Bihmidine et al, 2016). However, much remains to be clarified with respect to their particular roles in the phloem loading of Suc in photosynthetic tissues, its long-distance transport, and its unloading in sink tissues, especially in the grasses (Aoki et al, 2003;Braun and Slewinski, 2009;Bihmidine et al, 2013).…”

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confidence: 99%

Sucrose Transporter ZmSut1 Expression and Localization Uncover New Insights into Sucrose Phloem Loading

et al. 2016

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Tonoplast Sugar Transporters (SbTSTs) putatively control sucrose accumulation in sweet sorghum stems

Cited by 59 publications

References 36 publications

Transcriptome and metabolome reveal distinct carbon allocation patterns during internode sugar accumulation in different sorghum genotypes

Transcriptome and metabolome reveal distinct carbon allocation patterns during internode sugar accumulation in different sorghum genotypes

Roles of Aquaporins in Setaria viridis Stem Development and Sugar Storage

Sucrose Transporter ZmSut1 Expression and Localization Uncover New Insights into Sucrose Phloem Loading

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