<i>Tomato yellow leaf curl virus</i> Can Overwinter in <i>Stellaria aquatica</i>, a Winter-Hardy TYLCV-Reservoir Weed

Kil, Eui-Joon; Byun, Hee-Seong; Kim, Sunhoo; Cho, Seungchan; Cho, Sungrae; Roh, Kangsan; Lee, Kyeong-Yeoll; Choi, Hongsoo; Kim, Chang-Seok; Lee, Sukchan

doi:10.1094/pdis-04-14-0352-re

Cited by 12 publications

(6 citation statements)

References 22 publications

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“…These results suggest that winter annual weeds play important roles in overwintering of TSWV. From our previous study on overwintering of Tomato yellow leaf curl virus (TYLCV), it has been shown that S. aquatica can act as a viral source to newly introduced insect vectors in the next cultivation after overwintering in the non-heated greenhouse (Kil et al, 2015). We did not confirm overwintering of TSWV in wintering S. aquatica plants by time-course follow-up and arti- ficial experiments shown in the previous study on TYLCV, but it can be inferred that S. aquatica plants infected with TSWV play a similar role in overwintering and spread.…”

Section: Resultsmentioning

confidence: 99%

Life Cycle-Based Host Range Analysis for Tomato Spotted Wilt Virus in Korea

et al. 2020

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Tomato spotted wilt virus (TSWV) is one of the plant viruses transmitted by thrips and causes severe economic damage to various crops. From 2008 to 2011, to identify natural host species of TSWV in South Korea, weeds and crops were collected from 5 regions (Seosan, Yesan, Yeonggwang, Naju, and Suncheon) where TSWV occurred and were identified as 1,104 samples that belong to 144 species from 40 families. According to reverse transcription-polymerase chain reaction, TSWV was detected from 73 samples from 23 crop species, 5 of which belonged to family Solanaceae. Additionally, 42 weed species were confirmed as natural hosts of TSWV with three different life cycles, indicating that these weed species could play an important role as virus reservoirs during no cultivation periods of crops. This study provides up-to-date comprehensive information for TSWV natural hosts in South Korea.

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Section: Resultsmentioning

confidence: 99%

Life Cycle-Based Host Range Analysis for Tomato Spotted Wilt Virus in Korea

et al. 2020

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“…To confirm nucleotide sequences from amplicons from samples which showed positive results in previous PCR analyses, TYLCV IR was amplified by PCR with the IR-specific primer set (425 bp of target size) ( Kil et al, 2015 ). Amplicons were ligated into pGEM-T easy vectors (Promega, Madison, WI, USA) and sequenced (Macrogen, Seoul, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…Whitefly-mediated TYLCV transmission from TYLCV-infected E. indica plants to healthy tomato plants was performed in restricted conditions. Non-viruliferous Bemisia tabaci (the MED cryptic species) were bred with virus-free tomato plants and virus infection was checked by PCR as described in a previous study ( Kil et al, 2015 ). E. indica plants which were inoculated with TYLCV infectious clone and confirmed as TYLCV-infected were placed in three individual BugDorm-4E4590DH cages (MegaView Science Co.) together with three-week-old healthy “Seogwang” tomato (TYLCV-susceptible cultivar) plants and non-viruliferous whiteflies at 25°C with a day length of 12 h. A negative control group was also set with TYLCV-free E. indica , non-viruliferous whiteflies and healthy tomatoes in an insect cage.…”

Section: Methodsmentioning

confidence: 99%

Tomato Yellow Leaf Curl Virus Infection in a Monocotyledonous Weed (Eleusine indica)

et al. 2021

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Tomato yellow leaf curl virus (TYLCV) is one of the most important plant viruses belonging to the genus Begomovirus of the family Geminiviridae. To identify natural weed hosts that could act as reservoirs of TYLCV, 100 samples were collected at a TYLCV-affected tomato farm in Iksan from 2013 to 2014. The sample weeds were identified as belonging to 40 species from 18 families. TYLCV was detected in 57 samples belonging to 28 species through polymerase chain reaction using root samples including five species (Eleusine indica, Digitaria ciliaris, Echinochloa crus-galli, Panicum dichotomiflorum, and Setaria faberi) from the family Poaceae. Whitefly Bemisia tabaci-mediated TYLCV transmission from TYLCV-infected E. indica plants to healthy tomatoes was confirmed, and inoculated tomatoes showed typical symptoms, such as leaf curling and yellowing. In addition, TYLCV was detected in leaf and root samples of E. indica plants inoculated by both whitefly-mediated transmission using TYLCV-viruliferous whitefly and agro-inoculation using a TYLCV infectious clone. The majority of mastreviruses infect monocotyledonous plants, but there have also been reports of mastreviruses that can infect dicotyledonous plants, such as the chickpea chlorotic dwarf virus. No exception was reported among begomoviruses known as infecting dicots only. This is the first report of TYLCV as a member of the genus Begomovirus infecting monocotyledonous plants.

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“…Among the weed hosts for ToCV identified in this study, P. americana is a perennial weed and should be completely removed from inside or around tomato greenhouses to eliminate the continuous presence of ToCV. In our previous weed host survey of TYLCV, we identified and experimentally proved that Myosoton aquaticum, a perennial weed, could be a main TYLCV reservoir weed near tomato greenhouses and a source of consecutive TYLCV infections (Kil et al 2015). Non-viruliferous whitefly transmitted TYLCV from over-wintered TYLCV-infected M. aquaticum in spring and viruliferous whitefly transmitted the TYLCV to new tomato plants.…”

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confidence: 93%

Identification of natural weed hosts of Tomato chlorosis virus in Korea by RT-PCR with root tissues

et al. 2015

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Tomato chlorosis virus (ToCV) is a whiteflytransmitted crinivirus that causes interveinal chlorosis and bronzing on tomato leaves. Although outbreaks of ToCV have been reported in many countries, the virus was not reported in Korea until 2013. To identify weed hosts of ToCV that may serve as virus reservoirs, we analyzed various weeds that were growing together with ToCV-infected tomatoes and viruliferous Bemisia tabaci in a tomato greenhouse. We performed reverse transcription-polymerase chain reaction (RT-PCR) analysis of root samples from 148 samples of 61 species from 24 families of plants grown in the greenhouse to avoid possible ToCV contamination by whiteflies on leaves. Seventeen weed species were identified as ToCV hosts based on RT-PCR results of root samples: Conyza canadensis, Erigeron annuus, Sonchus asper, Youngia japonica, Trigonotis peduncularis, Cardamine flexuosa, Cerastium glomeratum, Stellaria media, Chenopodium album, Ipomoea hederacea, Quamoclit coccinea, Vicia angustifolia var. segetilis, V. tetrasperma, Phytolacca americana, Mazus pumilus, Solanum americanum and S. nigrum. The amplicons obtained by RT-PCR were confirmed as ToCV by sequence analysis. Life cycle analysis of the weeds indicated that each weed could play an important role as a Bgreen bridge^or virus reservoir between tomato cultivation seasons. This is the first report documenting the possible occurrence of ToCV in tomato plants based on weed life cycles.Eur J Plant Pathol

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Tomato yellow leaf curl virus Can Overwinter in Stellaria aquatica, a Winter-Hardy TYLCV-Reservoir Weed

Cited by 12 publications

References 22 publications

Life Cycle-Based Host Range Analysis for Tomato Spotted Wilt Virus in Korea

Life Cycle-Based Host Range Analysis for Tomato Spotted Wilt Virus in Korea

Tomato Yellow Leaf Curl Virus Infection in a Monocotyledonous Weed (Eleusine indica)

Identification of natural weed hosts of Tomato chlorosis virus in Korea by RT-PCR with root tissues

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