Tatumella ptyseos, an Unrevealed Causative Agent of Pink Disease in Pineapple

Abstract

Pink disease is a major problem in the pineapple canning industry. Affected fruit acquire a brownish pigment after pasteurization and can contaminate non-affected fruit before they are released to the consumer. In the last few years, Pantoea citrea has been described as the causative agent of pink disease. In this study, over 300 bacterial isolates from pineapple plants, growing in Mexican commercial fields, and from soil close to plant roots were recovered. Over 250 isolates showed a very high similarity in their phenotypic and genotypic traits with Tatumella ptyseos, a close relative of Pantoea. These isolates exhibited typical pathogenicity reactions in pineapple juice tests, pineapple slices and fruit. On this basis, molecular identification procedures for the Tatumella isolates associated with pink disease were implemented. In affected fruit populations around 10 6 CFU ⁄ g of fresh tissue were recovered. This is first time that T. ptyseos is demonstrated as a causal agent of pink disease.

The bacterial communities ofDrosophila suzukiicollected from undamaged cherries

“…Tatumella is an Enterobacteriaceae that has been linked to both human and plant infections (Costa, Mendes & Ribeiro, 2008; Marin-Cevada et al, 2010). Tatumella punctata, the nearest hit to the largest Tatumella OTU identified in this study, was originally isolated from oranges (Kageyama et al, 1992).…”

A Study of Pathogenic Fungi Causing Post Harvest Losses of Pineapple Sold at Wudil and Yan Lemo Markets of Kano State

“…Pineapples are subject to a variety of diseases, which include wilt disease, pink disease, bacterial heart rot, anthracnose, fungal heart rot, root rot, black rot, butt rot, fruit let core rot, and yellow spot virus [7,11]. Contamination of pine apples may take place at all stages during pre and post-harvest stages.…”

Tatumella saanichensis sp. nov., isolated from a cystic fibrosis patient

“…Clinical isolates of Tatumella ptyseos , a member of the family Enterobacteriaceae , are primarily obtained from patient sputa (Berkta et al , 2001; Hollis et al , 1981; Tan et al , 1989), and members of the genus Tatumella have been isolated previously from food and environmental samples (Brady et al , 2010; Garcia-Armisen et al , 2010; Ken et al , 2005; Marín-Cevada et al , 2010; Papalexandratou et al , 2011b). Phylogenetic studies using 16S rRNA gene sequence analysis suggest that T. ptyseos is closely related to bacteria of the genus Erwinia (Drancourt et al , 2001; Spröer et al , 1999), and comparative genetic analyses have demonstrated that T. ptyseos is also related to Morganella morganii and the genus Pantoea (Paradis et al , 2005; Brady et al , 2010).…”

“…Pairwise sequence alignments (shown in Table S2) were made between NML 06-3099 T and species reassigned to the genus Tatumella in 2010 (Brady et al , 2010), including (identity to the type strain): T. citrea (98.3 %), T. morbirosei (97.9 %), T. punctata (98.6 %) and T. terrea (98.7 %). Notably, the 16S rRNA gene sequence for NML 06-3099 T has been detected in separate sources of fruit in independent studies, including cocoa beans from Ecuador (Papalexandratou et al , 2011a) and Malaysia (Papalexandratou et al , 2013), pineapples from Mexico (Marín-Cevada et al , 2010) and a garden microbiome in Panama (GenBank accession no. HM556908).…”

“…nov., after the Saanich region of Vancouver Island, British Columbia, Canada. The prevalence and clinical importance of this novel species of the genus Tatumella is currently unknown, although notably, sequence for this bacterium had been detected by 16S rRNA gene sequencing in separate studies from fruit (Marín-Cevada et al , 2010; Papalexandratou et al , 2011a, 2013). Papalexandratou et al (2013) suggested that this species ( T. saanichensis sp.…”