<i>Taenia hydatigena</i>cysticercosis in wild boar (<i>Sus scrofa</i>) from southern Italy: an epidemiological and molecular survey

Sgroi, Giovanni; Varcasia, Antonio; D’Alessio, Nicola; Varuzza, Paolo; Buono, Francesco; Amoroso, Maria Grazia; Boufana, Belgees; Otranto, Domenico; Fioretti, Alessandro; Veneziano, Vincenzo

doi:10.1017/s0031182020001559

Cited by 13 publications

(11 citation statements)

References 47 publications

(62 reference statements)

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“…For nad5, of the 125 haplotypes, four haplotypes H8, H15, H51 and H47 appeared in both countries. For the spliced nad4-nad5 gene network, only one haplotype H76 of the 144 was common to both countries, and interstingly, similar ndings were reported previously (Sgroi et al, 2020). The majority of sequences from 93 out of 114 Chinese isolates were as haplogroup A variants, while 15 and 6 were as haplogroup B and A2 for the nad4 gene.…”

Section: Haplotype Networksupporting

confidence: 86%

Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia

Qurishi

Yan

et al. 2022

Parasitol Res

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Parasitic infection is one of the many challenges facing livestock production globally. Cysticercosis tenuicollis is a common parasitic disease in domestic and wild ruminants (intermediate host) caused by the larval stage of Taenia hydatigena primarily through infecting dogs (de nitive host). Although there are handful of genetic studies on this parasite, there is no available genetic data for the parasite in Mongolia. So, the main aim of this study was to identify the nuclear differences in ovine isolates of Cysticercus tanuicollis entering China from Mongolia and comparison with existing Chinese isolates from sheep and goats based on the recently described PCR-RFLP method and mitochondrial genes of NADH dehydrogenase subunit 4 (nad4) and the NADH dehydrogenase subunit 5 (nad5). Sixty nine isolates were collected during routine veterinary meat inspections from sheep that originated from Mongolia, at the modern Slaughterhouses in Erenhot City, Inner Mongolia. Additional 114 cysticerci were also retrieved from sheep and goats from northern (Inner Mongolia Autonomous Region, Ningxia Hui Autonomous Region and Gansu Province), western (Tibet Autonomous Region), and southern (Jiangxi Province and Guangxi Province) China. The PCR-RFLP approach of the nad5 showed nine mitochondrial clusters such as A1, A2, A3, A5, A8, A9, A10, A11 and B of T. hydatigena isolates from sheep and goats from Mongolia and China. Meanwhile, RFLP pro le of Haplogroup A1 was extensively spread than other Haplogroups. Therefore, these data provide supplementary information on the molecular epidemiology of T. hydatigena for China and Mongolia as animal movement between both countries is constantly occurring. This study also demonstrates that the genetic variants of T. hydatigena existing in China and Mongolia are closely similar. HighlightsCysticercosis caused by the larval stage of Taenia hydatigena is endemic in China and Mongolia.The pairwise xation index (Fst) showed the absence of genetic differentiation between sheep Cysticercus tenuicollis isolates from China and Mongolia for nad4 and concatenated nad4-nad5 mitochondrial gene sequences. Meanwhile, Fst for nad5 showed the presence of few genetic variation between sheep. However, C. tenuicollis from Chinese sheep were differentiated from goats.Our investigations for nad5 indicated the existence of nine mitochondrial clusters such as A1, A2, A3, A5, A8, A9, A10, A11 and B of T. hydatigena isolates from sheep and goats from China and Mongolia.

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Section: Haplotype Networksupporting

confidence: 86%

Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia

Qurishi

Yan

et al. 2022

Parasitol Res

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“…Therefore, the proximity, during hunting activities of dogs with wild animals, which are potential reservoirs of A. reconditum, could play an important epidemiological role in transmission (Diakou et al 2016). It should also be added that dogs used for hunting mammals (mainly wild boars) are maintained in packs of at least 4-5 animals (Sgroi et al 2020), a factor that can favour the spread of the ectoparasites among dogs reared together and thus could facilitate the transmission of A. reconditum.…”

Section: Discussionmentioning

confidence: 99%

Haematological and biochemical abnormalities in hunting dogs infected with Acanthocheilonema reconditum, associated risk factors, and a European overview

et al. 2021

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Acanthocheilonema reconditum is a filarial parasite transmitted by arthropods (fleas, lice, and ticks) that infect dogs. There is minimal published data available to date on potential haematological and biochemical changes associated with this parasitic infection. Study aims were (i) provide an overview of A. reconditum in Europe, (ii) define A. reconditum prevalence and risk factors in a specific dog population (hunting) from southern Italy, and (iii) assess the frequency of haemato-biochemical abnormalities associated with infection. Blood samples collected from 3020 dogs were tested by a modified Knott’s technique to count and identify microfilariae. Eighty-four dogs were infected by A. reconditum (2.78%; 95% CI 2.19–3.37%). Microfilariae ranged from 1 to 212/ml. Based on clinical examination, all but six dogs with non-specific symptoms were healthy. Haematological abnormalities included leucocytosis (n = 15), with eosinophilia (n = 14) and monocytosis (n = 13). Serum biochemical abnormalities included increased total serum proteins (n = 19), albumins (n = 7), total globulins (n = 14), ALT (n = 1), and ALP (n = 1); one dog was hypoalbuminemic, and BUN was mildly increased in 2 dogs. Risk factors included the province origin (Napoli, OR=5.4, 95%CI: 2.1–14.0; Caserta, OR=5.1, 95%CI: 2.5–10.6), hunting wild mammals (OR=2.8, 95% 95%CI: 1.6–4.8), and ectoparasite infestation (OR=1.9, 95%CI: 1.1–3.1). There was a negative correlation between microfilaraemic load and decreased albumin level (−0.37; p=0.021). Our results showed that A. reconditum circulates within the hunting dog population of southern Italy, with seemingly low pathogenic potential.

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“…Cysticercosis due to Taenia hydatigena metacestode formerly referred to as Cysticercus tenuicollis (Sgroi et al, 2020) is a widespread tapeworm infection of veterinary importance that infects domestic and wild ruminants. Taenia hydatigena uses canid (primarily dogs) as the definitive hosts which release eggs on pastures where grazing animals upon ingestion become infected.…”

Section: Introductionmentioning

confidence: 99%

A new molecular nomenclature for Taenia hydatigena: mitochondrial DNA sequences reveal sufficient diversity suggesting the assignment of major haplotype divisions

Ohiolei

Yan

et al. 2020

Parasitology

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Cysticercosis caused by the metacestode larval stage of Taenia hydatigena formerly referred to as Cysticercus tenuicollis is a disease of veterinary importance that constitutes a significant threat to livestock production worldwide, especially in endemic regions due to condemnation of visceral organs and mortality rate of infected young animals. While the genetic diversity among parasites is found to be potentially useful in many areas of research including molecular diagnostics, epidemiology and control, that of T. hydatigena across the globe remains poorly understood. In this study, analysis of the mitochondrial DNA (mtDNA) of adult worms and larval stages of T. hydatigena isolated from dogs, sheep and a wild boar in China showed that the population structure consists of two major haplogroups with very high nucleotide substitutions involving synonymous and non-synonymous changes. Compared with other cestodes such as Echinococcus spp., the genetic variation observed between the haplogroups is sufficient for the assignment of major haplotype or genotype division as both groups showed a total of 166 point-mutation differences between the 12 mitochondrial protein-coding gene sequences. Preliminary analysis of a nuclear protein-coding gene (pepck) did not reveal any peculiar changes between both groups which suggests that these variants may only differ in their mitochondrial makeup.

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Taenia hydatigenacysticercosis in wild boar (Sus scrofa) from southern Italy: an epidemiological and molecular survey

Cited by 13 publications

References 47 publications

Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia

Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia

Haematological and biochemical abnormalities in hunting dogs infected with Acanthocheilonema reconditum, associated risk factors, and a European overview

A new molecular nomenclature for Taenia hydatigena: mitochondrial DNA sequences reveal sufficient diversity suggesting the assignment of major haplotype divisions

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