<i>TaD27‐B</i> gene controls the tiller number in hexaploid wheat

Zhao, Bin; Wu, Ting; Shan, Shan; Jiang, Deng Ji; Bie, Xiao Min; Sui, Na; Zhang, Xian Sheng; Wang, Fang

doi:10.1111/pbi.13220

Cited by 79 publications

(50 citation statements)

References 56 publications

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“…However, it is important to recognize that increased tiller number is not likely to be beneficial to yield. Indeed, the aim of the new plant‐type rice ideotype was to have low tillering capacity and no unproductive tillers (Rubia et al , ).The recently published paper by Zhao et al () implicated wheat TaD27‐B (an orthologue of arabidopsis and rice D27, a SL biosynthetic enzyme) in mediating tiller number in wheat through modifying the size of the axillary buds. These authors suggest yield in wheat can be increased by manipulating SL biosynthesis (decreasing it) to increase tiller number.…”

Section: Associations Between Ckx and Seed Yield Componentsmentioning

confidence: 99%

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Chen

Zhao

Song

et al. 2019

Plant Biotechnology Journal

107

131

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J.S.)) and (Tel +64 275553582; email paula.jameson@canterbury.ac.nz (P.E.J.)) SummaryThe plant hormone group, the cytokinins, is implicated in both qualitative and quantitative components of yield. Cytokinins have opposing actions in shoot and root growth-actions shown to involve cytokinin dehydrogenase (CKX), the enzyme that inactivates cytokinin. We revise and provide unambiguous names for the CKX gene family members in wheat, based on the most recently released wheat genome database, IWGSC RefSeq v1.0 & v2.0. We review expression data of CKX gene family members in wheat, revealing tissue-specific gene family member expression as well as sub-genome-specific expression. Manipulation of CKX in cereals shows clear impacts on yield, root growth and orientation, and Zn nutrition, but this also emphasizes the necessity to unlink promotive effects on grain yield from negative effects of cytokinin on root growth and uptake of mineral nutrients, particularly Zn and Fe. Wheat is the most widely grown cereal crop globally, yet is under-research compared with rice and maize. We highlight gaps in our knowledge of the involvement of CKX for wheat. We also highlight the necessity for accurate analysis of endogenous cytokinins, acknowledging why this is challenging, and provide examples where inadequate analyses of endogenous cytokinins have led to unjustified conclusions. We acknowledge that the allohexaploid nature of bread wheat poses challenges in terms of uncovering useful mutations. However, we predict TILLING followed by whole-exome sequencing will uncover informative mutations and we indicate the potential for stacking mutations within the three genomes to modify yield components. We model a wheat ideotype based on CKX manipulation. 614

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Section: Associations Between Ckx and Seed Yield Componentsmentioning

confidence: 99%

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Chen

Zhao

Song

et al. 2019

Plant Biotechnology Journal

107

131

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“…The first strand cDNAs for qRT-PCR were synthesized using Synthesis SuperMix (TransGen, Beijing, China). The qRT-PCR amplifications were performed as described by Zhao et al [60]. Three biological replicates and three technical replicates were utilized.…”

Section: Expression Of the Tayth Genes Analyzed By Qrt-pcrmentioning

confidence: 99%

Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in common wheat

et al. 2020

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Background: N6-Methyladenosine (m6A) is the most widespread RNA modification that plays roles in the regulation of genes and genome stability. YT521-B homology (YTH) domain-containing RNA-binding proteins are important RNA binding proteins that affect the fate of m6A-containing RNA by binding m6A. Little is known about the YTH genes in common wheat (Triticum aestivum L.), one of the most important crops for humans. Results: A total of 39 TaYTH genes were identified in common wheat, which are comprised of 13 homologous triads, and could be mapped in 18 out of the 21 chromosomes. A phylogenetic analysis revealed that the TaYTHs could be divided into two groups: YTHDF (TaDF) and YTHDC (TaDC). The TaYTHs in the same group share similar motif distributions and domain organizations, which indicates functional similarity between the closely related TaYTHs. The TaDF proteins share only one domain, which is the YTH domain. In contrast, the TaDCs possess three C3H1-type zinc finger repeats at their N-termini in addition to their central YTH domain. In TaDFs, the predicated aromatic cage pocket that binds the methylysine residue of m6A is composed of tryptophan, tryptophan, and tryptophan (WWW). In contrast, the aromatic cage pocket in the TaDCs is composed of tryptophan, tryptophan, and tyrosine (WWY). In addition to the general aspartic acid or asparagine residue used to form a hydrogen bond with N 1 of m6A, histidine might be utilized in some TaDFb proteins. An analysis of the expression using both online RNA-Seq data and quantitative real-time PCR verification revealed that the TaDFa and TaDFb genes are highly expressed in various tissues/organs compared with that of TaDFcs and TaDCs. In addition, the expression of the TaYTH genes is changed in response to various abiotic stresses. Conclusions: In this study, we identified 39 TaYTH genes from common wheat. The phylogenetic structure, chromosome distribution, and patterns of expression of these genes and their protein structures were analyzed. Our results provide a foundation for the functional analysis of TaYTHs in the future.

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“…Moreover, SL biosynthesis in orchardgrass appears to be mainly controlled by two genes (CCD7 and MAX1) according to the above results, indicating that CCD7 and MAX1 are two key genes that in turn regulate the process of tillering in orchardgrass via the SL pathway. However, there is little information about the key regulatory genes underlying SL biosynthesis [60]. Hence, a thorough analysis of these two SL pathway genes is still required to determine their roles in detail.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)

Feng

Liang

et al. 2020

BMC Plant Biol

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Background: Tillering is an important agronomic trait underlying the yields and reproduction of orchardgrass (Dactylis glomerata), an important perennial forage grass. Although some genes affecting tiller initiation have been identified, the tillering regulatory network is still largely unknown, especially in perennial forage grasses. Thus, unraveling the regulatory mechanisms of tillering in orchardgrass could be helpful in developing selective strategies for high-yield perennial grasses. In this study, we generated high-throughput RNA-sequencing data from multiple tissues of tillering stage plants to identify differentially expressed genes (DEGs) between high-and low-tillering orchardgrass genotypes. Gene Ontology and pathway enrichment analyses connecting the DEGs to tillering number diversity were conducted. Results: In the present study, approximately 26,282 DEGs were identified between two orchardgrass genotypes, AKZ-NRGR667 (a high-tillering genotype) and D20170203 (a low-tillering genotype), which significantly differed in tiller number. Pathway enrichment analysis indicated that DEGs related to the biosynthesis of three classes of phytohormones, i.e., strigolactones (SLs), abscisic acid (ABA), and gibberellic acid (GA), as well as nitrogen metabolism dominated such differences between the high-and low-tillering genotypes. We also confirmed that under phosphorus deficiency, the expression level of the major SL biosynthesis genes encoding DWARF27 (D27), 9cis-beta-carotene 9′,10′-cleaving dioxygenase (CCD7), carlactone synthase (CCD8), and more axillary branching1 (MAX1) proteins in the high-tillering orchardgrass genotype increased more slowly relative to the low-tillering genotype.

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TaD27‐B gene controls the tiller number in hexaploid wheat

Cited by 79 publications

References 56 publications

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Genome-wide identification and expression analysis of YTH domain-containing RNA-binding protein family in common wheat

Comparative transcriptome analyses reveal different mechanism of high- and low-tillering genotypes controlling tiller growth in orchardgrass (Dactylis glomerata L.)

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