TACR1 gene polymorphism and sex differences in postoperative nausea and vomiting

Abstract

SummaryWe hypothesised that the genetic effect of single nucleotide polymorphisms in the TACR1 gene, which encodes NK1 receptors, could influence the sex difference in postoperative nausea and vomiting. Thirty-two selected single nucleotide polymorphisms were genotyped by the Sanger sequencing method in 200 patients who underwent lower abdominal surgery. The incidence and severity of postoperative nausea and vomiting were evaluated after surgery. The rs3755468-SNP showed significant association with the incidence and severity of postoperative nausea and vomiting (p = 0.016). The TT haplotype defined by two single nucleotide polymorphisms, including the rs3755468-SNP, was associated with reduced incidence and severity of postoperative nausea and vomiting in female patients (p = 0.03). The rs3755468-SNP is located within the predicted oestrogen response element and a DNase I hypersensitive site. The single nucleotide polymorphisms in the TACR1 gene are associated with sex differences in postoperative nausea and vomiting and may help to elucidate the mechanisms underlying these differences.

Long non-coding RNA MIR4300HG polymorphisms are associated with postoperative nausea and vomiting: a genome-wide association study

“…Contrary to expectation, non-smoking status, history of motion sickness, nitrous oxide use, and surgery duration were not identi ed as risk factors in this study. This observation is consistent with results of our previous study in a different Japanese cohort [8]. Surprisingly, fentanyl concentration was also not a PONV risk factor.…”

“…Furthermore, the multiple logistic regression analysis indicated a strong association between rs11232965-SNP in MIR4300HG and female sex with PONV incidence (Table 4). Female sex per se was previously identi ed as the strongest PONV risk factor [2], and our current and previous results [8] con rm this (the odds ratios were 14.7-fold and 7.15-fold, respectively). Sex is a genetic trait, and we speculate sex differences in PONV may involve variable regulation by lncRNAs or transcription factors.…”

Long non-coding RNA MIR4300HG polymorphisms are associated with postoperative nausea and vomiting: a genome-wide association study

“…Nevertheless, rs1333114-SNP variation was not a statistically significant genetic factor in the multiple logistic regression analysis (Table 4) Furthermore, the multiple logistic regression analysis indicated a strong association between rs11232965-SNP in MIR4300HG and female sex with PONV incidence (Table 4). Female sex per se was previously identified as the strongest PONV risk factor [2], and our current and previous results [8] confirm this (the odds ratios were 14.7-fold and 7.15-fold, respectively). Sex is a genetic trait, and we speculate sex differences in PONV may involve variable regulation by lncRNAs or transcription factors.…”

“…Indeed, our previous study showed polymorphism in TACR1 located where estrogen may act as a transcription factor was associated with PONV incidence and severity. [8] In this study no similar female-associated sequences were identified near rs1333114-SNP in PTPRD or rs11232965-SNP in MIR4300HG. Contrary to expectation, non-smoking status, history of motion sickness, nitrous oxide use, and surgery duration were not identified as risk factors in this study.…”

“…This observation is consistent with results of our previous study in a different Japanese cohort. [8] Surprisingly, fentanyl concentration was also not a PONV risk factor. As described in the results, there were no significant differences in blood fentanyl concentration between patients with and without PONV in either cohort.…”