<i>TaCol-B5</i>
            modifies spike architecture and enhances grain yield in wheat

Zhang, Xiaoyu; Jia, Haiyan; Li, Tian; Wu, Jizhong; Nagarajan, R.; Lei, Lei; Powers, Carol; Kan, Chia-Cheng; Wu, Hua Hsi; Liu, Zhiyong; Chen, Charles; Carver, Brett F.; Yan, Liuling

doi:10.1126/science.abm0717

Cited by 95 publications

(55 citation statements)

References 49 publications

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“…BBX is a transcription factor of zinc finger protein, which plays a prominent role in plant growth and development, hormone response, and stress tolerance [ 54 ], but the function of BBX genes in tobacco has not been studied. In this study, the phylogeny, gene structure, cis-acting element, and expression pattern under diverse conditions of the NtBBX genes were comprehensively analyzed in tobacco.…”

Section: Discussionmentioning

confidence: 99%

The Function of BBX Gene Family under Multiple Stresses in Nicotiana tabacum

Song

et al. 2022

Genes

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B-box (BBX) is a zinc finger transcription factor, which is involved in regulating the growth and development of plants and resisting various stresses. In this study, 43 NtBBX genes were identified and divided into five subgroups in tobacco. The members in each subgroup had similar characteristics. The promoter region of NtBBX genes had cis-acting elements related to light response, hormone regulation and stress response. Transcriptome analysis showed that NtBBX30 was significantly up-regulated, and NtBBX12, NtBBX13, NtBBX16 and NtBBX17 were significantly down-regulated under abiotic stresses. The NtBBX genes also responded to the infection of Ralstonia solanacearum. NtBBX9, NtBBX1, NtBBX15 and NtBBX17 showed the greatest response under stresses. The NtBBX genes are expressed in various degrees under different tissues. This research will provide a solid foundation for further study of the biological function of NtBBX genes in tobacco.

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Section: Discussionmentioning

confidence: 99%

The Function of BBX Gene Family under Multiple Stresses in Nicotiana tabacum

Song

et al. 2022

Genes

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“…The haplotype H5 was slightly sensitive to photoperiod in the 83B28 and A5302 backgrounds; the underlying mechanism deserves further investigation. In wheat, the CCT domain gene TaCol-B5 also has pleiotropic effects: It increases the number of spikelet nodes per spike, as well as the number of tillers and spikes, leading to increased grain yield ( Zhang et al, 2022 ). CCT domain genes have obvious convergent effects in rice ( Ghd7 ), maize ( ZmCCT ), and wheat ( TaCol-B5 ), all of which are pleiotropic genes that regulate plant growth, development, stress responses, yield performance, and other agronomic traits ( Xue et al, 2008 ; Weng et al, 2014 ; Zhang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

ZmCCT haplotype H5 improves yield, stalk-rot resistance, and drought tolerance in maize

et al. 2022

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The ZmCCT locus underlies both stalk-rot resistance and photoperiod sensitivity in maize (Zea mays L.). We previously introduced nine resistant ZmCCT haplotypes into seven elite but susceptible maize inbred lines (containing the haplotype H1) to generate 63 backcross families. Here, we continued backcrossing, followed by selfing, to develop 63 near-isogenic lines (NILs). We evaluated 22 of these NILs for stalk-rot resistance and flowering time under long-day conditions. Lines harboring the haplotype H5 outperformed the others, steadily reducing disease severity, while showing less photoperiod sensitivity. To demonstrate the value of haplotype H5 for maize production, we selected two pairs of NILs, 83B28H1/83B28H5 and A5302H1/A5302H5, and generated F1 hybrids with the same genetic backgrounds but different ZmCCT alleles: 83B28H1 × A5302H1, 83B28H1 × A5302H5, 83B28H5 × A5302H1, and 83B28H5 × A5302H5. We performed field trials to investigate yield/yield-related traits, stalk-rot resistance, flowering time, and drought/salt tolerance in these four hybrids. 83B28H5 × A5302H1 performed the best, with significantly improved yield, stalk-rot resistance, and drought tolerance compared to the control (83B28H1 × A5302H1). Therefore, the ZmCCT haplotype H5 has great value for breeding maize varieties with high yield potential, stalk-rot resistance, and drought tolerance.

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“…Induced novel mutations were also detected on other genes, such as TaTB1, TaQ, WFZP, Ms1, TaNAC019, TaCol-B5, and TaGW2, etc. (Dobrovolskaya et al, 2015;Wang et al, 2017b;Dixon et al, 2018;Li et al, 2019;Liu et al, 2020a;Zhang et al, 2022;Zhang et al), among which many mutation sites have not been reported before (Table S4). Thus, the generated KN9204 EMS-mutagenesis library contains novel allelic variations on yield and agronomical-traits related crucial genes and holds the potential for future breeding applications.…”

Section: Identification Of Gene-index Mutations In Kn9204 Mutant Librarymentioning

confidence: 99%

Boosting wheat functional genomics via indexed EMS mutant library of KN9204

Wang

et al. 2022

Preprint

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A better understanding of wheat functional genomics could facilitate the targeted breeding for agronomic traits improvement and environmental adaptation. With the release of reference genomes and extensive re-sequencing data of wheat and relatives, wheat functional genomics enters a new era. However, limited transformation efficiency in wheat hampers in-depth gene functional study and genetic manipulation for breeding. Here, we generated an EMS mutagenesis library of KN9204, a widely grown elite wheat variety in northern China, with available reference genome, transcriptome, and epigenome of various tissues. The library harbors enormous developmental diversity covering important tissues and transition stages. Exome capture sequencing of 2,090 mutant lines, with probes designed by KN9204 genome, revealed that 98.79% of coding genes have mutations and 1,383 EMS-type SNPs per line averagely. Novel allelic variations for important agronomic trait-related genes, such as Rht-D1, Q, TaTB1, and WFZP, were identified. We tested 100 lines with severe mutations in 80 NAC TFs under drought and salinity stresses, and found 13 lines with altered sensitivity. Three lines were further analyzed for the regulation insight of NAC TFs in stress response by combing transcriptome and available chromatin accessibility data. Hundreds of direct targets of NAC with altered transcriptional pattern in mutant lines under salt or drought stress induction were identified, including SNAC1, DREB2B, CML16 and ZFP182, knowing factors in abiotic stresses response. Thus, we have generated and indexed KN9204 EMS mutant library which would facilitate functional genomics study and provide resources for genetic manipulation in wheat.

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TaCol-B5 modifies spike architecture and enhances grain yield in wheat

Cited by 95 publications

References 49 publications

The Function of BBX Gene Family under Multiple Stresses in Nicotiana tabacum

The Function of BBX Gene Family under Multiple Stresses in Nicotiana tabacum

ZmCCT haplotype H5 improves yield, stalk-rot resistance, and drought tolerance in maize

Boosting wheat functional genomics via indexed EMS mutant library of KN9204

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