<i>T-brain</i>homologue (<i>HpTb</i>) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo

Fuchikami, Takuya; Mitsunaga‐Nakatsubo, Keiko; Amemiya, Shonan; Hosomi, Toshiya; Watanabe, Takashi; Kurokawa, Daisuke; Kataoka, Mikiko; Harada, Yasuji; Satoh, Nori; Kusunoki, Shinichiro; Takata, Koki; Shimotori, Taishin; Yamamoto, Takashi; Sakamoto, Naoaki; Shimada, Hiraku; Akasaka, Koji

doi:10.1242/dev.129.22.5205

Cited by 63 publications

(3 citation statements)

References 52 publications

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“…The GRN regulating PMC specification and skeletogenesis is well characterized in the sea urchin, with Alx1, Ets1/2, and Tbr known to be key TFs of the skeletogenic GRN ( Kenny et al, 1999 ; Kurokawa et al, 1999 ; Croce et al, 2001 ; Fuchikami et al, 2002 ; Oliveri et al, 2002 ; 2008 ; Ettensohn et al, 2003 ; Howard-Ashby et al, 2006 ; Rizzo et al, 2006 ; Revilla-i-Domingo et al, 2007 ; Khor et al, 2019 ). Knockdown of Alx1, Ets1/2, and Tbr yielded predicted phenotypes of PMC and skeletal loss ( Supplementary Figure S1B ).…”

Section: Resultsmentioning

confidence: 99%

“…In the sea urchin, Tbr is zygotically expressed in the skeletogenic mesoderm of the cleavage and blastula stage embryo ( Croce et al, 2001 ; Oliveri et al, 2002 ). Tbr is involved in skeletogenic mesoderm specification, as well as skeletogenesis in the larva, with Tbr loss-of-function resulting in complete loss of the larval skeleton ( Croce et al, 2001 ; Fuchikami et al, 2002 ; Hinman et al, 2007 ; Gao and Davidson, 2008 ; Oliveri et al, 2008 ). We have previously found that Sp miR-1 overexpression results in mispatterning of the skeletogenic cells and duplicated branching of the larval skeleton ( Sampilo and Song, 2024 ).…”

Section: Discussionmentioning

confidence: 99%

“…Transcription factors and signaling pathways important for vascularization are expressed and utilized in the sea urchin skeletogenic cells (primary mesenchyme cells; PMCs) at the time of migration and patterning, and in skeletal formation ( Duboc et al, 2004 ; Oliveri et al, 2008 ; Morgulis et al, 2019 ). Prior work has shown that Ets1/2, Tbr, and Alx1 are all key regulators of sea urchin skeletogenesis ( Fuchikami et al, 2002 ; Ettensohn et al, 2003 ; Oliveri et al, 2008 ). Of these, we found Sp miR-1 to suppress reporters containing 3′UTRs of Ets1/2 and Tbr ( Sampilo and Song, 2024 ) and Sp miR-31 to suppress reporters containing 3′UTR of Alx1 and VegfR7 ( Stepicheva and Song, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors

Arnott,

Sampilo,

Song

2024

Front. Cell Dev. Biol.

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In early embryonic development, the cross-regulation of transcription factors and signaling pathways are critical in mediating developmental and physiological processes. Additionally, many studies have shown the importance of post-transcriptional regulation of signaling and network components mediated by microRNAs (miRNAs); however, how miRNAs are transcriptionally regulated is poorly understood. miRNAs are critical fine-tuners of many biological processes and their dysregulation leads to a variety of diseases and developmental defects. Previously, we have shown that miRNAs are dynamically expressed throughout sea urchin development, suggesting that miRNAs are likely to be under transcriptional regulation. Here, we used pharmacological inhibitors, genetic constructs, and loss-of-function reagents to assess the impact of key signaling pathways (Wnt, Nodal, MAPK, Sonic Hedgehog, Delta/Notch, VEGF, and BMP) and transcription factors (Alx1, Ets1/2, and Tbr) on the transcript levels of the evolutionarily conserved miR-1, miR-31, miR-92 and miR-124; the invertebrate-specific miR-71; and the echinoderm-specific miR-2002, miR-2007, and miR-2012. We also used computational methods to identify potential transcription factor binding sites of these miRNAs. Lists of binding motifs for transcription factors (TFs) were acquired from the MEME-Suite Motif Database and used as inputs for the algorithm FIMO (Find Individual Motif Occurrences), which detects short nucleotide motifs within larger sequences. Based on experimental data on miRNA expression in conjunction with bioinformatic predictions, we propose that the transcription factors Tbr, Alx1, and Ets1 regulate SpmiR-1, SpmiR-31, and SpmiR-71, respectively. We additionally observed significant effects on miRNA levels as a result of perturbations to Wnt, Nodal, MAPK, and Sonic Hedgehog signaling pathways, while no significant change on miRNA levels were observed with perturbations to Delta/Notch, VEGF, or BMP signaling pathways. Overall, this study provides insights into the transcriptional regulation of miRNAs by signaling pathways and transcription factors and contribute to our overall understanding of the genetic regulation of developmental processes.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors

Arnott,

Sampilo,

Song

2024

Front. Cell Dev. Biol.

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The Morphogenesis and Biomineralization of the Sea Urchin Larval Skeleton

Wilt¹,

Ettensohn²

2007

Handbook of Biomineralization

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Nucleocytoplasmic functions of the PDZ‐LIM protein family: new insights into organ development

et al. 2010

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Recent work on the PDZ-LIM protein family has revealed that it has important activities at the cellular level, mediating signals between the nucleus and the cytoskeleton, with significant impact on organ development. We review and integrate current knowledge about the PDZ-LIM protein family and propose a new functional role, sequestering nuclear factors in the cytoplasm. Characterized by their PDZ and LIM domains, the PDZ-LIM family is comprised of evolutionarily conserved proteins found throughout the animal kingdom, from worms to humans. Combining two functional domains in one protein, PDZ-LIM proteins have wide-ranging and multi-compartmental cell functions during development and homeostasis. In contrast, misregulation can lead to cancer formation and progression. New emerging roles include interactions with integrins, T-box transcription factors, and receptor tyrosine kinases. Facilitating the assembly of protein complexes, PDZ-LIM proteins can act as signal modulators, influence actin dynamics, regulate cell architecture, and control gene transcription.Keywords: actin cytoskeleton; organogenesis; PDZ-LIM; scaffold; T-box Evolutionarily conserved building blocks for a heterogeneous protein familyThe PDZ-LIM family is comprised of proteins with multiple functional domains, and all share a PDZ domain combined with at least one LIM domain. As protein-protein interaction modules, PDZ and LIM domains act as scaffolds, binding to filamentous actin-associated proteins, a range of cytoplasmic signaling molecules, and nuclear proteins, allowing this family to carry out diverse functions during development and adulthood.( Figure 1 shows the architecture of the PDZ-LIM protein subfamilies and their phylogenetic relationship. While both the PDZ and the LIM domains are often found in combination with other peptide motifs or domains, the coevolution of the two invariant functional domains, PDZ and LIM, indicate high functional relevance and, thus, is of particular interest and the focus of this review. All family members associate with the actin cytoskeleton, and this property, organizing protein complexes at the cytoskeleton, may serve a range of unexpected, yet important biological roles. PDZ domainFound in bacteria, yeast, and throughout the animal kingdom, the PDZ domain is one of the most common protein-protein binding domains. (17,19) It is characterized by a highly conserved sequence of 80-90 amino acids consisting of

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T-brainhomologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo

Cited by 63 publications

References 52 publications

Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors

Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors

The Morphogenesis and Biomineralization of the Sea Urchin Larval Skeleton

Nucleocytoplasmic functions of the PDZ‐LIM protein family: new insights into organ development

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