sucABandsucCDare mutually essential genes inEscherichia coli

Yu, Byung Jo; Sung, Bong Hyun; Lee, Ju Young; Son, Seungwoo; Kim, Mi Sun; Kim, Sun Chang

doi:10.1111/j.1574-6968.2005.00026.x

Cited by 31 publications

(18 citation statements)

References 23 publications

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“…sucAB and sucCD encode the enzymes, 2-oxoglutarate dehydrogenase and succinyl coenzyme A synthetase, that generate succinyl-CoA from 2-oxoglutarate and succinate, respectively. Besides its central role in the TCA cycle, succinyl-CoA is also used as an intermediate in many metabolic pathways, a major one being the lysine biosynthetic pathway, where succinyl-CoA is used to generate diaminopimelate that is required for the biosynthesis of peptidoglycan [66].…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Analysis of the Pho Regulon in a pstCA Mutant of Citrobacter rodentium

et al. 2012

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The phosphate-specific transport operon, pstSCAB-phoU, of Gram-negative bacteria is an essential part of the Pho regulon. Its key roles are to encode a high-affinity inorganic phosphate transport system and to prevent activation of PhoB in phosphate-rich environments. In general, mutations in pstSCAB-phoU lead to the constitutive expression of the Pho regulon. Previously, we constructed a pstCA deletion mutant of Citrobacter rodentium and found it to be attenuated for virulence in mice, its natural host. This attenuation was dependent on PhoB or PhoB-regulated gene(s) because a phoB mutation restored virulence for mice to the pstCA mutant. To investigate how downstream genes may contribute to the virulence of C. rodentium, we used microarray analysis to investigate global gene expression of C. rodentium strain ICC169 and its isogenic pstCA mutant when grown in phosphate-rich medium. Overall 323 genes of the pstCA mutant were differentially expressed by at least 1.5-fold compared to the wild-type C. rodentium. Of these 145 were up-regulated and 178 were down-regulated. Differentially expressed genes included some involved in phosphate homoeostasis, cellular metabolism and protein metabolism. A large number of genes involved in stress responses and of unknown function were also differentially expressed, as were some virulence-associated genes. Up-regulated virulence-associated genes in the pstCA mutant included that for DegP, a serine protease, which appeared to be directly regulated by PhoB. Down-regulated genes included those for the production of the urease, flagella, NleG8 (a type III-secreted protein) and the tad focus (which encodes type IVb pili in Yersinia enterocolitica). Infection studies using C57/BL6 mice showed that DegP and NleG8 play a role in bacterial virulence. Overall, our study provides evidence that Pho is a global regulator of gene expression in C. rodentium and indicates the presence of at least two previously unrecognized virulence determinants of C. rodentium, namely, DegP and NleG8.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Analysis of the Pho Regulon in a pstCA Mutant of Citrobacter rodentium

et al. 2012

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“…In the article by Yu et al (2005), the authors Byung Jo Yu and Bong Hyun Sung should have been credited as contributing equally to the work. We apologise for this error.…”

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confidence: 99%

sucABandsucCDare mutually essential genes inEscherichia coli

Sung

Lee

et al. 2006

FEMS Microbiology Letters

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“…Phophoenolpyruvate carboxylase catalyses carboxylation of pyruvate to oxaloacetate and succinyCOA ligase catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate [61], [62]. The genes encoding putative haloacid dehalogenase like hydrolase (pfhB) and xylose isomerase ( xylA ) were also down-regulated, which could be because of the absence of the substrates for these enzymes in BALF or because alternate pathways are preferable in that environment.…”

Section: Resultsmentioning

confidence: 99%

Modulation of Gene Expression in Actinobacillus pleuropneumoniae Exposed to Bronchoalveolar Fluid

et al. 2009

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Background Actinobacillus pleuropneumoniae, the causative agent of porcine contagious pleuropneumonia, is an important pathogen of swine throughout the world. It must rapidly overcome the innate pulmonary immune defenses of the pig to cause disease. To better understand this process, the objective of this study was to identify genes that are differentially expressed in a medium that mimics the lung environment early in the infection process.Methods and Principal FindingsSince bronchoalveolar lavage fluid (BALF) contains innate immune and other components found in the lungs, we examined gene expression of a virulent serovar 1 strain of A. pleuropneumoniae after a 30 min exposure to BALF, using DNA microarrays and real-time PCR. The functional classes of genes found to be up-regulated most often in BALF were those encoding proteins involved in energy metabolism, especially anaerobic metabolism, and in cell envelope, DNA, and protein biosynthesis. Transcription of a number of known virulence genes including apxIVA and the gene for SapF, a protein which is involved in resistance to antimicrobial peptides, was also up-regulated in BALF. Seventy-nine percent of the genes that were up-regulated in BALF encoded a known protein product, and of these, 44% had been reported to be either expressed in vivo and/or involved in virulence.ConclusionsThe results of this study suggest that in early stages of infection, A. pleuropneumoniae may modulate expression of genes involved in anaerobic energy generation and in the synthesis of proteins involved in cell wall biogenesis, as well as established virulence factors. Given that many of these genes are thought to be expressed in vivo or involved in virulence, incubation in BALF appears, at least partially, to simulate in vivo conditions and may provide a useful medium for the discovery of novel vaccine or therapeutic targets.

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sucABandsucCDare mutually essential genes inEscherichia coli

Cited by 31 publications

References 23 publications

Genome-Wide Analysis of the Pho Regulon in a pstCA Mutant of Citrobacter rodentium

Genome-Wide Analysis of the Pho Regulon in a pstCA Mutant of Citrobacter rodentium

sucABandsucCDare mutually essential genes inEscherichia coli

Modulation of Gene Expression in Actinobacillus pleuropneumoniae Exposed to Bronchoalveolar Fluid

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