Streptomyces nodosusHost Strains Optimized for Polyene Glycosylation Engineering

Polyene macrolide biosynthesis in streptomycetes and related bacteria: recent advances from genome sequencing and experimental studies

“…The perDII perosamine synthase and perDI perosaminyltransferase genes have been cloned from the perimycin producer Streptomyces cacaoi (Hutchinson et al, 2010). Complementation of amphDII mutations with a perDII perosamine synthase gene led to formation of perosaminyl-amphoteronolides (Hutchinson et al, 2010;Stephens et al, 2012). Increased yields of the perosaminyl analogue were obtained by including an AmphDI-PerDI hybrid glycosyltransferase (GT) capable of recognising the amphotericin aglycone and GDP--Dperosamine (Hutchinson et al, 2010).…”

Exploiting the genome sequence of Streptomyces nodosus for enhanced antibiotic production

“…This drug is the most effective treatment for life-threatening systemic 3 mycoses, but has severe side effects. A few less toxic analogues have been obtained by engineering of biosynthetic genes (Stephens et al, 2012;De Poire et al, 2013). Medicinal chemists have also generated promising derivatives by modifying amphotericin B isolated from fermentation cultures of the producer organism (Yamamoto et al, 2015;Davis et al, 2015;Volmer et al, 2010).…”

Engineered Biosynthesis of Disaccharide-Modified Polyene Macrolides

“…Several new analogs have been biosynthesized by genetic manipulation of the amphotericin B-producing microorganism Streptomyces nodosus. These new analogs have been obtained by reprogramming the polyketide synthase and by engineering late genes required for modification of the macrolactone core (5,6,7). Biosynthetic engineering has generated analogs of other important polyene macrolides, such as nystatin, candicidin, pimaricin, and rimocidin (8,9,10,11).…”