Streptomyces: A superior source for cholesterol oxidase used in serum cholesterol assay

Lolekha, Porntip H.; Jantaveesirirat, Yaovalak

doi:10.1002/jcla.1860060612

Cited by 24 publications

(12 citation statements)

References 5 publications

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“…[7]. Cho from Streptomyces species has been reported to be superior to those from other microorganisms on account of lower cost of production, stability and longer shelf life [8]. Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Praveen

Srivastava

Tripathi

2011

Appl Biochem Biotechnol

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An extracellular cholesterol oxidase (cho) enzyme was isolated from the Streptomyces parvus, a new source and purified 18-fold by ion exchange and gel filtration chromatography. Specific activity of the purified enzyme was found to be 20 U/mg with a 55 kDa molecular mass. The enzyme was stable at pH 7.2 and 50 °C. The enzyme activity was inhibited in the presence of Pb(2+), Ag(2+), Hg(2+), and Zn(2+) and enhanced in the presence of Mn(2+). The enzyme activity was inhibited by the thiol-reducing reagents (DTT, β-mercaptoethanol), suggesting that disulfide linkage is essential for the enzyme activity. The enzyme activity was found to be maximum in the presence of Triton X-100 and X-114 detergents whereas sodium dodecyl sulfate fully inactivated the enzyme. The enzyme showed moderate stability towards all organic solvents except acetone, benzene, chloroform and the activity increased in the presence of isopropanol and ethanol. The K(m) value for the oxidation of cholesterol by this enzyme was 0.02 mM.

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Section: Introductionmentioning

confidence: 99%

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Praveen

Srivastava

Tripathi

2011

Appl Biochem Biotechnol

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“…During past three decades, cholesterol oxidases (COX) have been purified from several microorganisms, such as Brevibacterium (Gadda et al 1997), gamma-Proteobacterium (Isobe et al 2003), Nocardia (Richmond 1973), Pseudomonas (Doukyu and Aono 1998), Rhodococcus (Sojo et al 2002), Bacillus (Kim et al 2002), Schizophyllum and Streptomyces (Gadda et al 1997;Lolekha and Jantaveesirirat 1992). The cholesterol oxidases from different microorganism exhibit many distinct properties and not much is known about the properties in practical clinical use.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of extracellular cholesterol oxidase from Enterobacter sp.

Lei

et al. 2008

World J Microbiol Biotechnol

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The objective of this work is to obtain an abundant source of cholesterol oxidases for industrial and medicinal needs. Thirteen bacterial strains that express high level of inducible extracellular cholesterol oxidase (COX) were isolated from carnivore feces. One of these strains, named COX8-9, belonging to the genus Enterobacter, was found to produce the highest level of cholesterol oxidase. COX from strain COX8-9 was purified from the culture supernatant by ultrafiltration followed with two consecutive Q-Sepharose chromatographies at different pH values, and then by Superdex-75 gel filtration. The purified enzyme was a monomer with a molecular weight of 58 kDa, and exhibited maximum absorption at 280 nm. The K m value for oxidation of cholesterol by this enzyme was 1.2 9 10 -4 M, with optimum activity at pH 7.0. Enzymatic activity of COX was enhanced 3-fold in the presence of metal ion Cu 2+ , and the enzyme was stable during long-term aqueous storage under various temperatures, indicating its potential as a clinical diagnostic reagent. Preparation and characterization of cholesterol oxidases from the other selected strains are under way.

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“…Actinomyces lavendulae [4], Streptomyces fradiae [5], Streptomyces sp. [6]. Streptomyces are potent producers of valuable antibiotics and are also non-pathogenic in nature.…”

Section: Coxs Have Been Isolated and Purified From Various Micro-orgamentioning

confidence: 99%

Artificial Neural Network Modeling to Predict the Non-Linearity in Reaction Conditions of Cholesterol Oxidase from Streptomyces olivaceus MTCC 6820

Sahu¹,

Shera²,

Banik³

2019

JBM

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Cholesterol oxidase (COX) is widely used enzyme for total cholesterol estimation in human serum and for the fabrication of electro-chemical biosensors. COX is also used for the bioconversion of cholesterol; for the production of precursors of steroidal drugs and hormones. Enzyme activity depends decisively on defined conditions with respect to pH, temperature, ionic strength of the buffer, substrate concentration, enzyme concentration, reaction time. Standardization of these parameters is desirable to attain optimum activity of the enzyme. The present work aims to build a neural network model using five input parameters (pH, cholesterol concentration, 4-aminoantipyrine concentration, crude COX volume and horseradish peroxidase) and one output i.e., COX activity (U/ml) as a response. A feed forward back propagation neural network with Levenberg-Marquardt training algorithm was used to train the network. The network performance was assessed in terms of regression (2 R), Mean Squared Error (MSE) and Mean Absolute Percentage Error (MAPE). A network topology of 5-10-1 was found to be optimum. The MSE, MAPE and 2 R values of the neural model were 0.0075%, 0.12% and 0.9792% respectively. The maximum predicted activity of COX was 1.073 U/ml, which was close to the experimental value i.e., 1.1 U/ml at simulated optimum assay conditions. MSE and MAPE depicted the precision in the prediction efficiency of the developed ANN model. Higher 2 R value showed a good correlation between the experimental and ANN predicted values. This proved the robustness of the ANN model to predict similar type of system (COX from other Streptomyces sp.) within the limits of the trained data set. The COX activity was enhanced by 1.71 folds after optimization of the reaction conditions.

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Streptomyces: A superior source for cholesterol oxidase used in serum cholesterol assay

Cited by 24 publications

References 5 publications

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Purification and characterization of extracellular cholesterol oxidase from Enterobacter sp.

Artificial Neural Network Modeling to Predict the Non-Linearity in Reaction Conditions of Cholesterol Oxidase from <i>Streptomyces olivaceus</i> <i>MTCC</i> 6820

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Streptomyces: A superior source for cholesterol oxidase used in serum cholesterol assay

Cited by 24 publications

References 5 publications

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Purification and characterization of extracellular cholesterol oxidase from Enterobacter sp.

Artificial Neural Network Modeling to Predict the Non-Linearity in Reaction Conditions of Cholesterol Oxidase from &lt;i&gt;Streptomyces olivaceus&lt;/i&gt; &lt;i&gt;MTCC&lt;/i&gt; 6820

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Artificial Neural Network Modeling to Predict the Non-Linearity in Reaction Conditions of Cholesterol Oxidase from <i>Streptomyces olivaceus</i> <i>MTCC</i> 6820