<i>Streptococcus pneumoniae–</i>Induced Oxidative Stress in Lung Epithelial Cells Depends on Pneumococcal Autolysis and Is Reversible by Resveratrol

Zahlten, Janine; Kim, Yeji; Doehn, Jan-Moritz; Pribýl, T; Hocke, Andreas C.; García, Pedro; Hammerschmidt, Sven; Suttorp, Norbert; Hippenstiel, Stefan; Hübner, Ralf-Harto

doi:10.1093/infdis/jiu806

Cited by 58 publications

(43 citation statements)

References 30 publications

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“…In addition, it plays a critical role in inflammatory responses in lung diseases through the upregulation of redox-sensitive transcription factors, resulting in enhanced expression of proinflammatory genes. [19][20][21][22] This results in extensive cellular and tissue damage and contributes significantly to worsening of lung infection responses by decreasing the antioxidant capacity. [19][20][21][22] In this way, oxidative stress can affect remodeling of the extracellular matrix, mitochondrial apoptosis, cell proliferation, alveolar repair, and immune modulation.…”

Section: Discussionmentioning

confidence: 99%

“…[19][20][21][22] This results in extensive cellular and tissue damage and contributes significantly to worsening of lung infection responses by decreasing the antioxidant capacity. [19][20][21][22] In this way, oxidative stress can affect remodeling of the extracellular matrix, mitochondrial apoptosis, cell proliferation, alveolar repair, and immune modulation. [20][21][22][23] Therefore the test of TAS, TOS, and OSI level may useful for distinguishing exudates and transudates in PPEs.…”

Section: Discussionmentioning

confidence: 99%

“…[19][20][21][22] In this way, oxidative stress can affect remodeling of the extracellular matrix, mitochondrial apoptosis, cell proliferation, alveolar repair, and immune modulation. [20][21][22][23] Therefore the test of TAS, TOS, and OSI level may useful for distinguishing exudates and transudates in PPEs.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

What is the Diagnostic Value of Plasma Antioxidative/Oxidative Status in Parapneumonic Effusions in Children?

Aydinoğlu¹,

Cevik²,

Böleken³

et al. 2017

Turkiye Klinikleri J Med Sci

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A AB BS ST TR RA AC CT T O Ob bj je ec ci iv ve e: : Pleural effusions are associated with high morbidity in children. Oxidative stress, which refers to an imbalance between oxidants and antioxidants, is associated with various diseases including lung diseases. The aim of this study was to investigate to the total oxidative status (TOS) and total antioxidative status (TAS) in differentiating exudates from transudates in children. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : In this cross-sectional study, data from patients who were hospitalized for parapneumonic effusions and underwent thoracentesis from October 2012 to December 2013 in the pediatric surgery department. The patiens were divided into two groups (the exuda and transude groups) according to Light's criteria. Blood plasma and parapneumonic effusions were analyzed and compared with respect to the total antioxidative and oxidative status. R Re es su ul lt ts s: : The study consisted of blood plasma and pleural effusion from 40 children with parapneumonic effusions (PPEs) (transude group [n=20]; exuda group [n=20]) and just blood plasma from 30 healthy children (control group). There were no significant difference between the groups in terms of gender and age. The blood plasma TAS level of the control group was significantly higher than the level of blood plasma TAS level of the exuda and transude groups with statistically significant (p<0.001). The value of plasma oxidative stress index (OSI) and TOS of exuda group were significantly higher than those of the control and transude groups with statistically significant (p<0.05). C Co on nc cl lu us si io on n: : This study suggests that inflammation is associated with PPE in children that increased OSI in blood plasma from PPE patients is related to tissue damage and turnover. K Ke ey yw wo or rd ds s: : Child; exudates and transudates Ö ÖZ ZE ET T A Am ma aç ç: : Plevral efüzyonlar çocuklarda yüksek morbidite ile ilişkilidir. Oksidatif stres, oksidanlar ile antioksidanlar arasındaki dengesizliği ifade eder ve akciğer hastalıkları dahil biçok hastalıkla ilişkilidir. Bu çalışmanın amacı, çocuklarda transüda ve eksüdaların ayırıcı tanısında toplam oksidatif durumun (TOS) ve total antioksidatif durumun (TAS) yararlılığını araştırmaktır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Bu çalışmada kesitsel olarak Ekim 2012'den Aralık 2013'e kadar parapnömonik efüzyon nedeniyle çocuk cerrahisi bölümüne başvuran ve torasentez yapılan hastalardan elde edilen veriler sunulmuştur. Light kriterlerine göre hastalar iki gruba ayrıldı (eksudatif ve transudatif). Kan plazması ve parapnömonik efüzyonlar analiz edilip ve toplam antioksidatif ve oksidatif duruma göre karşılaştırıldı. B Bu ul lg gu ul la ar r: : Bu çalışma, 30 sağlıklı çocuğun (kontrol grubu) kan plazmasını, 40 parapnömonik efüzyonlu (PPE) hastanın; (transüda grubu [n=20], eksüda grubu [n=20]) kan plazmasını ve pleural efüzyonu içermektedir. Cinsiyet ve yaş açısından gruplar arasında anlamlı fark yoktu. Kontrol grubunun kan plazma TAS...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

What is the Diagnostic Value of Plasma Antioxidative/Oxidative Status in Parapneumonic Effusions in Children?

Aydinoğlu¹,

Cevik²,

Böleken³

et al. 2017

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

show abstract

“…Besides this oxidative stress in lungs which is directly attributable to the pneumococci [317], the massive recruitment of polymorphonuclear leukocytes in pneumococcal pneumonia and subsequent respiratory burst of these cells may further impact on junctional dysfunction in vivo [328].…”

Section: Alteration Of Cell Junction Proteins In Pneumococciinfected mentioning

confidence: 99%

“…Resveratrol is an antioxidant which has been shown to induce intracellular gluthathione synthesis in lung epithelial cells [339] and is able to prevent pneumococci-related oxidative stress induction in airway epithelial cells [317].…”

Section: Conclusion and Further Outlookmentioning

confidence: 99%

Studies on cell junctions in an ex vivo human lung infection model

et al. 2015

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Yields and Immunomodulatory Effects of Pneumococcal Membrane Vesicles Differ with the Bacterial Growth Phase

Mehanny

Kroniger

Koch

et al. 2021

Adv Healthcare Materials

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Streptococcus pneumoniae infections are a leading cause of death worldwide. Bacterial membrane vesicles (MVs) are promising vaccine candidates because of the antigenic components of their parent microorganisms. Pneumococcal MVs exhibit low toxicity towards several cell lines, but their clinical translation requires a high yield and strong immunogenic effects without compromising immune cell viability. MVs are isolated during either the stationary phase (24 h) or death phase (48 h), and their yields, immunogenicity and cytotoxicity in human primary macrophages and dendritic cells have been investigated. Death-phase vesicles showed higher yields than stationary-phase vesicles. Both vesicle types displayed acceptable compatibility with primary immune cells and several cell lines. Both vesicle types showed comparable uptake and enhanced release of the inflammatory cytokines, tumor necrosis factor and interleukin-6, from human primary immune cells. Proteomic analysis revealed similarities in vesicular immunogenic proteins such as pneumolysin, pneumococcal surface protein A, and IgA1 protease in both vesicle types, but stationary-phase MVs showed significantly lower autolysin levels than death-phase MVs. Although death-phase vesicles produced higher yields, they lacked superiority to stationary-phase vesicles as vaccine candidates owing to their similar antigenic protein cargo and comparable uptake into primary human immune cells.

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Streptococcus pneumoniae–Induced Oxidative Stress in Lung Epithelial Cells Depends on Pneumococcal Autolysis and Is Reversible by Resveratrol

Abstract: These observations indicate a H2O2-independent induction of oxidative stress in lung epithelial cells via the release of bacterial factors of S. pneumoniae. Resveratrol might be an option for prevention of acute lung injury and inflammatory responses observed in pneumococcal pneumonia.

Cited by 58 publications

References 30 publications

What is the Diagnostic Value of Plasma Antioxidative/Oxidative Status in Parapneumonic Effusions in Children?

What is the Diagnostic Value of Plasma Antioxidative/Oxidative Status in Parapneumonic Effusions in Children?

Studies on cell junctions in an ex vivo human lung infection model

Yields and Immunomodulatory Effects of Pneumococcal Membrane Vesicles Differ with the Bacterial Growth Phase

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