Stewartia koreanaextract stimulates proliferation and migration of human endothelial cells and induces neovasculizationin vivo

Abstract

Angiogenesis, the growth of new blood vessels from preexisting vasculature, plays an important role in physiological and pathological processes such as embryonic development and wound healing. This study investigated the effects of methanol extracts of Stewartia koreana leaves (SKE) on angiogenesis. Stewartia koreana significantly promoted the proliferation and migration of human umbilical vein endothelial cells in a dose-dependent manner. The SKE induced endothelial cell proliferation in the range of 50 microg/mL without cytotoxicity. Treatment of HUVECs resulted in the activation of the mitogen-activated protein kinases that was correlated with endothelial cell proliferation and migration. SKE also stimulated angiogenesis in a chick chorioallantoic membrane assay, demonstrating promotion of new blood vessel formation in vivo. Local administration of SKE onto skin punched wounds resulted in increased von Willebrand Factor antigen, indicating that it stimulated neovasculization in the wound region. The results suggest that Stewartia koreana extracts may potentially be useful for the development of agents to accelerate vascular wound healing or to promote the growth of collateral blood vessels in ischemic tissues.

Inhibitory effects of a spinasterol glycoside on lipopolysaccharide-induced production of nitric oxide and proinflammatory cytokines via down-regulating MAP kinase pathways and NF-κB activation in RAW264.7 macrophage cells

“…e l s e v i e r . c o m / l o c a t e / i n t i m p inhibition of NO production in LPS-stimulated macrophage cells, and inhibition of HIV protease activity [18][19][20]. A number of phytosterols such as α-amyrin, isoquercitrin, and 3-O-β-D-glucopyanosylspinasterol (spinasterol-Glc) were isolated from methanol extracts of S. koreana leaves.…”

Ethanol Extract of Sambucus formosana Stimulates HUVEC Proliferation and Tube Formation through MEK1/2, ERK1/2 Pathway

“…Globally accepted herbal drugs are thought to be safe and effective, however, more evidence-based confirmation studies are needed. Among the most frequently studied proangiogenic phytochemicals are ginsenosides from Panax ginseng [1], beta-sitosterol from Aloe vera [2], calycosin from Radix Astragali [4], saponins from Panax notoginseng [5] and extracts from Hippophae rhamnoides L. [2], Radix Astragali [6], Puerariae flos [7], Stewartia koreana [8] and Angelica sinensis [9]. Those previous studies are suggested Chinese medicines as formula for treating angiogenesis disease and wound healing.…”

Does Propolis Help to Maintain Oral Health?

“…It was also found that single components of propolis do not have the same antiviral effect as their mixture in the form of bee glue. This is the basis for the conclusion that propolis extracts can be used locally in viral infections [59]. The research done by Shimizu et al indicates that propolis delays growth and progression of skin changes in an early stage of infection with Herpes simplex and does not cause cytotoxic effect [60].…”